Melatonin Extraction and Determination by UHPLC-FD from Different Plants

Yıl 2024, Cilt: 3 Sayı: 2, 220 - 228, 18.10.2024

Yusuf Güllüoğlu Enes Yararlı , Ahmet Murat Ahmet Turan Tay Yılmaz Uğur , Zeynep Maraş Selim Erdoğan

Öz

Many plants have become a piece of the traditional food as human diet and evaluated in folk medicine to treat diseases. They are very rich in terms of nutraceuticals. Melatonin functions as a free radical scavenger and controls the regulation of the sleep–wake cycle in mammals. In this study, we analyzed melatonin content in the herba parts of Stachys lavandulifolia, Tribulus terrestris, and Helichrysum arenarium, and unripe fruits of Prunus armeniaca L., Prunus cerasifera, and Prunus amygdalus L. A solvent mixture of methanol:ultrapure water:HCl (70:29.9:01 v/v/v) was used for the extraction of melatonin from plant samples. The determination of melatonin in the extracts was carried out by high pressure liquid chromatography with fluorescence detector (UHPLC-FD). Melatonin concentration was 0.0231 mg/kg for herba of Helichrysum arenarium and 0.0018 mg/kg for Prunus armeniaca L. Melatonin was not detected in other plant samples.

Anahtar Kelimeler

Helianthus annuus, Prunus armeniaca L., Melatonin

Kaynakça

• Verde A, Miguez JM, Leao-Martins JM, Gago-Martinez A, Gallardo M. Melatonin Content in Walnuts and Other Commercial Nuts. Influence of Cultivar, Ripening and Processing (roasting). Journal of Food Composition and Analysis, 2022; 104180.
• Arnao MB, Ruiz JH. Melatonin in plants. Plant Signaling & Behavior, 2007; 2: 381-382.
• Fracassetti D, Vigentini I, Lo Faro AFF, De Nisi P, Foschino R, Tirelli A, Orioli M, Iriti M. Assessment of Tryptophan, Tryptophan Ethylester, and Melatonin Derivatives in Red Wine by SPE-HPLC-FL and SPE-HPLC-MS Methods. Food, 2019; 8: 99.
• Varga-Visi E, Jocsak I, Loki Katalin, Vegvari K. Analysis of melatonin and serotonin from cherries: a review. Acta Agraria Kaposvariensis, 2018; 22: 52-60.
• Pranil T, Moongngarm A, Manwiwattanakul G, Loypimai PL, Kerr W. Melatonin and its derivative contents in tropical fruits and fruit tablets. Journal of Food Composition and Analysis, 2021; 103: 104109.
• Escrivá L, Manyes L, Barberà M, Martínez-Torres D, Meca G. Determination of melatonin in Acyrthosiphon pisum aphids by liquid chromatography-tandem mass spectrometry. Journal of Insect Physiology, 2016; 86: 48–53.
• Meng X, Li Y, Li S, Zhou Y, Gan RY, Xu DP, Li HB. Dietary sources and bioactivities of melatonin. Nutrients, 2017; 9: 367.
• Zia-Ul-Haq M, Ahmad S, Qayum M, Ercisli S. Compositional studies and antioxidant potential of Albizia lebbeck (L.) Benth. Pods and seeds. Turkish Journal of Biology. 2013; 37: 25-32.
• King, F.B. Interpreting wild food plants in archaeological record. In: Etkin NL, editor. Eating on the wild side. Tucson: University of Arizona Press; 1994.
• Hernández-Ruiz J, Arnao MB. Distribution of melatonin in diferent zones of lupin and barley plants at diferent ages in the presence and absence of light. Journal of Agricultural and Food Chemistry, 2008; 56:10567–10573.
• Burkhardt S, Tan DX, Manchester LC et al. Detection and quantification of the antioxidant melatonin in Montmorency and Balaton tart cherries (Prunus cerasus). Journal of Agricultural and Food Chemistry, 2001; 49: 4898 – 4902.
• Dubbels R, Reiter RJ, Klenke E et al. Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry. Journal of Pineal Research, 1995; 18: 28 – 31.
• Murch SJ, Saxena PK. Mammalian neurohormones: potential significance in reproductive physiology of St. John's Wort (Hypericum perforatum L.). Naturwissenschaften, 2002; 89: 555–560.
• Manchester LC, Tan DX, Reiter RJ et al. High levels of melatonin in the seeds of edible plants. Possible function in germ tissue protection. Life Sciences, 2000; 67: 3023 – 3029.
• Uğur Y. Investigation of melatonin content and antioxidant capacity in grape berries. Journal of Inonu University Health Services Vocational School, 2021, 3:820-830.
• Arabsalehi F, Rahimmalek M, Ehtemam MH. Phytochemical and morphological variation of Stachys lavandulifolia Vahl. populations as affected by genotype × year interaction. Industrial Crops and Products, 2018; 342-352.
• Rabbani M, Sajjadi SE, KarimiFirouzjaei M, Ghanadian M. Bioactivity guided isolation of apigenin from Stachys lavandulifolia Vahl. in mice with anxiolytic effects. Journal of Herbmed Pharmacology, 2018; 74-78.
• Khoigani SR, Rajaei A, Goli SAH. Evaluation of antioxidant activity, total phenolics, total flavonoids and LC-MS/MS characterisation of phenolic constituents in Stachys lavandulifolia. Natural Product Research, 2017; 355-358.
• Hammoda HM, Ghazy NM, Harraz FM, Radwan MM, ElSohly MA, Abdallah II. Chemical constituents from Tribulus terrestris and screening of their antioxidant activity. Phytochemistry, 2013; 153-155.
• Pljevljakušić D, Bigović D, Janković T. Biologically active compounds of Helichrysum arenarium (L.) Moench. European Journal of Drug Metabolism and Pharmacokinetics, 1999; 309-313.
• Wu Y, Zhong P. Clinical progress on management of pneumonia due to COVID19 with Chinese traditional patent medicines. Frontiers in Pharmacology, 2021; 12: 655063.
• Özkarakaş İ, Ercan N. Güneydoğu Anadolu ve Karadeniz Bölgelerinden Toplanan Bazı Erik (Prunus cerasifera Ehrh.) Genetik Kaynakları Materyalinin Değerlendirilmesi. Anadolu Ege Tarımsal Araştırma Enstitüsü Dergisi, 2016; 13(1): 91-106.
• Abdullah MK, Hussain MK. Badam (Prunus amygdalus Bail.): A fruit with medicinal properties. International Journal of Herbal Medicine, 2017; 5(5): 114-117.
• Rao H. Therapeutic applications of almonds (Prunus amygdalus L.): a review. Journal Of Clinical And Diagnostic Research. 2012; 6(1): 130-135.
• Esfahlan AJ, Jamei R, Esfahlan RJ. The importance of almond (Prunus amygdalus L.) and its by-products. Food Chemistry, 2010; 120(2): 349-360.
• Uğur Y, Zengin R, Ernim C, Günhan Zİ, Şalva E, Erdoğan S. Changes in the Phenolic, Melatonin, Sugar Contents and Antioxidant Capacity, Depending on Ripening Stage in Different Cornelian Cherry (Cornus mas L.) Fruits. ChemistrySelect, 2024; 9: e202304682. https://doi.org/10.1002/slct.202304682
• Feng X, Wang M, Zhao Y, Han P, Dai Y. Melatonin from different fruit sources, functional roles, and analytical methods. Trends in Food Science and Technology, 2014; 37(1): 21-31.
• Uğur Y. Extraction and quantification of melatonin in cornelian cherry (Cornus mas L.) by ultra-fast liquid chromatography coupled to fluorescence detector (UFLC-FD). Acta Chromatographica, 2023; 35(3): 219-226. https://doi.org/10.1556/1326.2022.01052
• Fracassetti D, Vigentini I, Lo Faro AFF, De Nisi P, Foschino R, Tirelli A, Orioli M, Iriti M. Assessment of Tryptophan, Tryptophan Ethylester, and Melatonin Derivatives in Red Wine by SPE-HPLC-FL and SPE-HPLC-MS Methods. Foods, 2019; 8(3): 99. https://doi.org/10.3390/foods8030099
• Arnao MB, Hernández-Ruiz J. Melatonin in Plants: More Studies are Necessary. Plant Signal Behavior, 2007; 2(5): 381-382. doi:10.4161/psb.2.5.4260
• Chen G, Huo Y, Tan DX, Liang Z, Zhang W, Zhang Y. Melatonin in Chinese medicinal herbs. Life Sciences, 2003; 73: 19-26.
• Kolar J, Malbeck J. Levels of the antioxidant melatonin in fruits of edible berry species. Planta Medica, 2009; 75(09): PJ42.
• Meng X, Li Y, Li S, Zhou Y, Gan RY, Xu DP, Li HB. Dietary sources and bioactivities of melatonin. Nutrients, 2017; 9: 367.
• Burkhardt S, Tan DX, Manchester LC, Hardeland R, Reiter RJ. 2001. Detection and quantification of the antioxidant melatonin in Montmorency and Balaton tart cherries (Prunus cerasus). Journal of Agricultural and Food Chemistry, 2001; 49: 4898–4902.
• González-Gómez D, Lozano M, Fernández-León MF. 2009. Detection and quantification of melatonin and serotonin in eight Sweet Cherry cultivars (Prunus avium L.). European Food Research and Technology, 2009; 229: 223–229.