Phylogenetic Analysis and Genetic Characterization of Bovine Respiratory Syncytial Virus G Glycoprotein / Bovine Respiratory Syncytial Virus G Glycoproteininin Filogenetik ve Analizi ve Genetik Karakterizasyonu

Yıl 2024, Cilt: 3 Sayı: 2, 32 - 39, 23.12.2024

Ali Küçük , Özge Sevinç Korkmaz Akar , Yakup Yıldırım , Kamil Atlı

Öz

This study aimed to molecularly detect Bovine Respiratory Syncytial Virus (BRSV) in cattle with clinical signs of respiratory system infection and to conduct a phylogenetic analysis of the detected strains. To detect the pathogen, nasal swab samples collected from 96 cattle were subjected to Reverse Transcriptase – nested PCR using specific primer sets. As a result of the molecular techniques, viral nucleic acid was detected in 2.08% (2/96) of the samples. Additionally, sequence and phylogenetic analyses of the detected local variant were performed and compared with various BRSV strains identified in different regions of Turkey and around the world. The genetic data obtained revealed that the isolates identified in this study belong to Subgroup III, alongside BRSV strains reported from various regions of Turkey, France, and the USA. Immunologically significant point mutations were detected in the immunodominant G glycoprotein region of the variant (Asn(N)179 → Asp(D) / Ala(A) 205 → Thr (T)). This result indicates that BRSV strains circulating among cattle can escape the host immune system without losing their pathogenicity and is considered to be one of the important factors that can cause respiratory system infection outbreaks in cattle.

Anahtar Kelimeler

“Cattle”, “BRSV”, “RT-nPCR”, “Genetic Characterization”

Etik Beyan

Approval was obtained from the animal testing local ethics committee of Burdur Mehmet Akif Ersoy University. The procedures used in this study adhere to the tenets of the Declaration of Helsinki (MAKU-HADYEK 1045/2023)

Destekleyen Kurum

The authors did not receive support from any organization for the submitted work.

Phylogenetic Analysis and Genetic Characterization of Bovine Respiratory Syncytial Virus G Glycoprotein

Öz

Bu araştırmada solunum sistemi enfeksiyonu klinik bulguları gösteren sığırlarda Bovine Respiratory Syncytial Virus (BRSV)’ un moleküler tespiti ve tespit edilen lokal suşların filogenetik analizlerinin gerçekleştirilmesi hedeflenmiştir. Etken tespiti için 96 sığırdan toplanan nazal sürüntü örneklerine spesifik primer setleri kullanılarak Reverz Transkriptaz-nested PCR (RT-nPCR) yöntemi uygulanmıştır. Uygulanan moleküler teknikler sonucunda % 2.08 (2/96) oranında viral nükleik asit tespiti gerçekleştirildi. Bununla birlikte tespit edilen lokal suşların sekans ve filogenetik analizleri gerçekleştirilerek Türkiye ve Dünya’nın farklı bölgelerinden tespit edilmiş farklı BRSV suşları ile karşılaştırmaları yapıldı. Elde edilen genetik verilerden, bu çalışmada tespit edilen izolatların, Türkiye, Fransa ve ABD’ nin farklı bölgelerinden bildirilen BRSV suşları ile birlikte, Subgrup III içerisinde yer aldığı belirlenmiştir. Ayrıca izolatların immünodominant G glikoprotein bölgesinde immünolojik açıdan önemli nokta mutasyonları tespit edilmiştir (Asn(N)179 → Asp(D) / Ala(A) 205 → Thr (T)). Bu sonuç, sığırlar arasında dolaşan BRSV suşlarının patojenitelerini kaybetmeden konak bağışıklık sisteminden kaçabildiklerini ve sığırlarda solunum sistemi enfeksiyon salgınlarına neden olabilecek önemli faktörlerden biri olarak değerlendirilmiştir.

Anahtar Kelimeler

“Cattle”, “BRSV”, “RT-nPCR”, “Genetic Characterization”

Kaynakça

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