INVESTIGATION OF THE STABILIZATION OF CAMPTOTHECIN ANTICANCER DRUG VIA PSA-PEG POLYMERIC PARTICLES

Year 2016, Volume: 17 Issue: 1, 221 - 231, 25.04.2016

Dilay Kizisar N. Tuna Subasi , Serap Eroksuz Ayhan S. Demir Olcay Mert

https://doi.org/10.18038/btda.87862

Abstract

Nano ve mikro taşıyıcı oluşumu için biyolojik olarak parçalanabilen PSA:PEG kopolimerlerinin sentezleri gerçekleştirilmiştir. Kamptotesin (CPT), antikanser ilaç olarak seçilmiştir. CPT fizyolojik koşullar altında kolay hidrolize olabilir (pH=7.4). Bu aktivite kaybına yol açar ve ilaç aktif lakton formundan aktif olmayan zehirli karboksilat forma dönüşür. Bu çalışmada anti kanser ilacı lakton formunda tutmak için, CPT etkili bir şekilde PSA:PEG nanopartikül ve mikropartiküllere yüklenmiştir ve taşıyıcı içindeki CPT kararlılığı detaylı bir şekilde HPLC ile incelenmiştir. Nano ve mikro taşıyıcılar içinde ilacın yüksek derecede kararlı ve aktif biçimde olduğu bulunmuştur (> % 95). Son olarak parçacıklar konfokal, SEM ve optik mikroskoplar ile görüntülenmiştir

Investigation of The Stabilization of Camptothecin Anticancer Drug via PSA-PEG Polymeric Particles

Abstract

Syntheses of biodegradable PSA:PEG copolymers for the formation of nano and micro carriers were performed. Camptothecin (CPT) was selected as anti-cancer drug. CPT can easily hydrolyze at physiological conditions (pH=7.4). This causes the loss of its activity, and it turns into inactive toxic carboxylate form from active lactone state. To keep anti cancer drug in the lactone form, it was efficiently loaded into PSA:PEG nanoparticles and microparticles, and the stability of CPT in the carriers was fully examined with HPLC. It was found that the drug was highly stable and in active form in the prepared nano and microcarriers (>95 %). Particles were imaged with confocal, SEM, and optic microscopes.

Keywords

Camptothecin, PSA-PEG, Stability, Nanoparticles

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