SİVAS’IN SOLAR TUZLALARINDAKİ MİKROBİYAL TOPLULUKLARIN KÜLTÜRBAĞIMLI KARAKTERİZASYONU

Yıl 2018, Cilt: 7 Sayı: 1, 1 - 11, 28.02.2018

Mehmet Burçin Mutlu , Seval Çınar

https://doi.org/10.18036/aubtdc.305855

Öz

Bu çalışmada Sivas’ta bulunan farklı solar tuzlalardan halofilik mikroorganizmaların izolasyonu yapılmıştır ve elde edilen izolatların 16S rRNA gen analizine dayalı karakterizasyonu gerçekleştirilmiştir. İzolasyon işlemi farklı tuz konsantrasyonları ile hazırlanmış 5 farklı besiyeri ile gerçekleştirilmiştir. Morfolojik olarak farklılık gösteren kolonilerin 16S rRNA genleri ARDRA ile analiz edilmiştir ve kesim profilleri karşılaştırılmıştır. 47 adet suş tanımlama amacıyla seçilmiştir. Bacteria domaini içerisinde yer alan izolatların Idiomarina, Salinivibrio, Halomonas, Salinibacter, Gracilibacillus, Thalassobacillus, Aliifodinibius, Chromohalobacter, Planococcus, Marinobacter, Bacillus, Aquisalimonas, Marinococcus, Alkalibacillus, Kangiella ve Microbulbifer cinsleri ile filogenetik olarak ilişkili olduğu saptanmıştır. Halofilik arke izolatları ise Haloferax, Natrinema, Haloterrigena, Halopelagius, Halorhabdus ve Haloarcula cinsleri içerisinde dağılım göstermiştir.

Anahtar Kelimeler

Halofilik Mikroorganizmalar, Solar Tuzlalar, Sivas

CULTIVATION-BASED CHARACTERIZATION of MICROBIAL ASSEMBLAGES in SOLAR SALTERNS, SİVAS

Öz

In this study, halophilic microorganisms were isolated from different solar salterns in Sivas and characterization of these isolates based on 16S rRNA gene analysis have been performed. Isolation was done by using 5 different media prepared with different salt concentrations. 16S rRNA genes of morphologically distinct colonies were analyzed by ARDRA and restriction profiles were compared. 47 strains were selected for identification purposes. It has been determined that the isolates in the Bacteria domain are phylogenetically related to genera Idiomarina, Salinivibrio, Halomonas, Salinibacter, Gracilibacillus, Thalassobacillus, Aliifodinibius, Chromohalobacter, Planococcus, Marinobacter, Bacillus, Aquisalimonas, Marinococcus, Alkalibacillus, Kangiella and Microbulbifer. Halophilic archaeal isolates were distributed among genera Haloferax, Natrinema, Haloterrigena, Halopelagius, Halorhabdus and Haloarcula.

Anahtar Kelimeler

Halophilic Microorganisms, Solar Salterns, Sivas

Kaynakça

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