Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups

Yıl 2012, Cilt: 65 Sayı: 1, 39 - 46, 01.01.2012

Esma Söylemez Zeliha Kayaaltı , Vugar Aliyev Tülin Söylemezoğlu

https://doi.org/10.1501/Tipfak_0000000805

Öz

Anahtar Kelimeler

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Dokuz comet parametresine göre kadın ve erkek gruplarda sigara içiminin DNA hasarına etkisi

Öz

Amaç: Sigara kullanımı, halk sağlığı için ciddi bir tehdit olușturmaktadır. Çalıșmamızın amacı,
sigara içimi ile lenfosit hücrelerinde DNA hasarı arasındaki ilișkiyi araștırmaktır. Sigaranın potansiyel
genotoksik etkisi, “comet length” (CL), “comet intensity” (CI), “head length” (HL), “head
intensity” (HI), “tail length” (TL), “tail intensity” (TI), “DNA tail” (DNAt), “tail moment” (TM) ve
olive tail moment (OTM) gibi dokuz “comet assay” parametresi ile analiz edilmiștir. İlk kez bu
çalıșmada sigara içenler kadın ve erkek olarak gruplara ayrılmıș ve dokuz comet parametresine
göre değerlendirme yapılmıștır.
Materyal ve Metod: 120 gönüllü birey (60 sigara içmeyen, 60 sigara içen) kan lenfosit hücrelerindeki
DNA hasarları açısından izlenmiștir. DNA hasar dereceleri BAB Bs Comet Assay sistemi ile
ölçülmüștür.
Bulgular: Sigara içen ve içmeyen gruplar arasında CI, TL ve OTM comet parametreleri açısından
yüksek derecede anlamlı ilișki bulundu (p < 0.01). Sigara içen kadın grubunun DNA hasarı açısından
CL, CI, HL, HI, TL, TI (p < 0.01) ve TM (p < 0.05) parametreleri sigara içmeyen kadın grubuna
kıyasla daha yüksektir. Buna karșın, sigara içen ve içmeyen erkek grupları arasında sadece DNAt
ve OTM parametreleri açısından istatistiksel anlamlı fark gözlenmiștir (p < 0.05). Kadınlardan
alınan tüm kan örneklerinin sigara indeksi (SI), tüm çalıșılan comet parametrelerine dayanarak
karșılaștırıldığında, TM dıșındaki bütün parametreler ile istatistiksel olarak anlamlı ilișki bulunmuștur.
Diğer taraftan, erkek grubundaki kan örneklerinde CL, HL, HI, TI ve OTM parametrelerinde
istatistiksel anlamlılık gözlenmiștir (p < 0.05).
Sonuç: Netice olarak, sigaranın DNA hasarlarına sebep olduğu ve kadınların, sigaranın zararlı
etkilerine karșı daha duyarlı olduğu söylenilebilir.

Anahtar Kelimeler

Comet Assay, DNA hasarı, Sigara içimi

Kaynakça

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