Ordu İli Yaprak Lahana Ekim Alanlarından İzole Edilen Rhizoctonia Grubu Fungusların Anastomozis Gruplarının, Karakteristik Özelliklerinin ve Patojenisitelerinin Belirlenmesi

Yıl 2020, Cilt: 9 Sayı: 2, 229 - 238, 30.12.2020

Halil İbrahim Benli , Muharrem Türkkan

https://doi.org/10.29278/azd.695494

Öz

2017-2018 vejetasyon döneminde Ordu ilinin on bir ilçesi (Akkuş, Altınordu, Aybastı, Çatalpınar, Çaybaşı, Fatsa, Perşembe, Gölköy, Gülyalı, Gürgentepe ve Ulubey)’nden hastalıklı yaprak lahana bitkileri ve onların rizosfer toprağından toplam elli dört Rhizoctonia spp. izolatı elde edilmiştir. Rhizoctonia spp. izolatlarının kültürel özellikleri, anastomozis grupları ve patojenisiteleri incelenmiştir. Bu izolatların %62.96’sının binükleik (BN) Rhizoctonia spp. (AG-A, AG-E, AG-F, AG-K ve AG-O) ve %37.34’ünün multinükleik (MN) Rhizoctonia solani (AG 1, AG 2-1, AG 4 ve AG 5) olduğu tespit edilmiştir. Sörvey yapılan tüm arazilerde en yaygın grup, 22 izolat ile BN Rhizoctonia spp. AG-A olarak belirlenmiştir. Geri kalan 32 izolatın 10’u R. solani AG 4, 6’sı BN Rhizoctonia spp. AG-K, 4’ü R. solani AG 2-1, dördü BN Rhizoctonia spp. AG-F, 3’ü R. solani AG 5, 1’er tanesi R. solani AG 1, BN Rhizoctonia AG-E ve AG-O’ya ait olduğu belirlenmiştir. Tüm izolatların %53.70’i kullanılarak yaprak lahana fideleri üzerinde yürütülen patojenisite testlerinde, genel olarak BN ve MN Rhizoctonia spp. izolatları arasında istatistiksel olarak önemli virülens farklılıkları olduğu tespit edilmiştir (P<0.05). BN Rhizoctonia spp. izolatlarının hastalık şiddeti skalası değerlerinin 0.00 ile 2.83 arasında değişirken, MN R. solani izolatlarının hastalık şiddeti skalası değerlerinin 2.17 ile 3.00 arasında değiştiği belirmiştir. Ayrıca, hastalık şiddeti skala değerleri arasında hiçbir önemli bir fark bulunmayan AG 4, AG 2-1, AG-F, AG-E, AG 1 ve AG 5 izolatları, kontrol bitkileri ile kıyaslandığında bitki büyüme parametleri (bitki boyu, gövde ve kök yaş ve kuru ağırlıkları ve kök uzunluğu)’ni önemli ölçüde azaltmıştır (P<0.05).

Anahtar Kelimeler

Yaprak lahana, Rhizoctonia spp., kök çürüklüğü, anastomozis grup, patojenisite

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Kaynakça

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