Mikronükleus (MN) ve Comet Assay testlerinin farklı hayvan türlerindeki uygulamalarına örnekler

Yıl 2024, Cilt: 26 Sayı: 1, 332 - 344, 19.01.2024

Gülşah Kurucu , Derya Bostancı

https://doi.org/10.25092/baunfbed.1353319

Öz

Farklı hayvanların dokularında biriken pek çok kirletici faktör canlıda genotoksik etki göstermektedir. Bu etkinin değerlendirilmesinde kullanılan genotoksisite testleri; kirlilik ajanlarının hayvanlarda DNA hasarına neden olup olmadığının belirlenmesine olanak sağlar. Bu faktörlerin etkilerini belirleyebilmek için farklı hayvan dokularında pek çok test sistemi geliştirilmiş ve genotoksisite testleri büyük önem kazanmıştır. Bu çalışmada, genotoksik hasarın belirlenmesinde çok yaygın olarak kullanılan iki test yöntemi olan Mikronükleus ve Comet Assay testleri karşılaştırılmış, avantaj ve dezavantajları belirlenerek farklı hayvan türlerinde yapılan çalışmaların değerlendirilmesi amaçlanmıştır.

Anahtar Kelimeler

Genotoksisite, Genotoksik Test Yöntemleri, MN Testi, Comet Assay

Examples of the applications of Micronucleus (MN) and Comet Assay tests in different animal species.

Öz

Many pollutants accumulate in the tissues of various animals and exhibit genotoxic effects in living organisms. Genotoxicity tests, used to assess this effect, allow for the determination of whether pollutants cause DNA damage in the organism. To determine the effects of these factors, various test systems have been developed in different animal tissues, and genotoxicity tests have gained significant importance. In this study, two widely used test methods for assessing genotoxic damage, Micronucleus and Comet Assay tests, were compared, their advantages and disadvantages were determined, and the evaluation of studies conducted in different animal species was aimed.

Anahtar Kelimeler

Genotoxicity, Genotoxic Test Methods, MN Test, Comet Assay

Kaynakça

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