Dondurulmuş Embriyo Transfer Edilmiş İneklerde Diöstrüste GnRH Enjeksiyonu ya da Progesterone İmplantının Etkisi

Yıl 2014, Cilt: 1 Sayı: 1-2, 1 - 7, 10.04.2015

Mesut Kırbaş , Bülent Bülbül Mehmet Köse Şükrü Dursun Mehmet Çolak

Öz

Bu çalışmada, dondurulmuş-çözdürülmüş embriyo transferi yapılmış ineklerde 13. gün (östrüs=0) tek doz GnRH ya da 13-20. günler arasında progesteron uygulamasının kan plazması progesteron seviyesi ve gebelik oranları üzerine etkisi araştırıldı. Bu amaçla, 48 baş İsviçre Esmeri ırkı inek senkronize edilerek taşıyıcı olarak kullanıldı. Östrüs senkronizasyon protokolünü takiben tespit edilen östrüsten 7 gün sonra bütün ineklere ethilen glikolle dondurulmuş-çözdürülmüş embriyolar transfer edildi ve inekler rastgele üç gruba ayrıldı. GnRH grubunda (n=16), ineklere 13. gün 10 g buserelin asetat i.m. yolla enjekte edildi. İmplant grubundaki (n=16) ineklere 3 mg norgestomet içeren implantlar kulak derisi altına yerleştirildi. Kontrol grubundaki (n=16) ineklere ise 13. gün i.m. olarak 2 ml fizyolojik tuzlu su solusyonu enjekte edildi. İmplant grubunda 16 ve 20. günlerde saptanan progesteron seviyeleri GnRH ve Kontrol gruplarından yüksek oldu (p<0.05). Yirmisekizinci gün gebelik oranları GnRH, İmplant ve Kontrol gruplarında sırasıyla %43.75 (7/16), %50 (8/16) ve %18.75 (3/16) olarak tespit edildi ve İmplant grubunda Kontrol grubundan yüksek olarak bulundu (p=0.06). Sonuç olarak, ineklerde östrüsten sonraki 13-20. günler arasında kulak implantı şeklinde progesteron uygulaması 16 ve 20. günlerde progesteron seviyesini yükseltti ve kontrol grubuna göre gebelik oranını artırdı

Anahtar Kelimeler

GnRH, kulak implantı, plazma progesteron, gebelik, embriyo transfer, inek

The Effect of a GnRH Agonist Injection or Progesterone Implant at Diestrus in Cryopreserved Embryo Transferred Cows

Öz

In this study, the effect of a single dose of GnRH on d 13 or progesterone implant for 7 days between d 13 and 20 on plasma progesterone levels and pregnancy rates on cryopreserved embryo transferred cows were investigated. Synchronized 48 Brown Swiss recipient cows were used as animal material. Seven days after estrus detection, cryopreserved cattle embryos were transferred into recipients and cows were assigned randomly into three groups. In GnRH group (n=16), cows were intramuscularly injected 10 g buserelin acetate on d 13. In Implant group (n=16), ear implants containing 3 mg norgestomet were applied for 7 days between d 13 and 20. Control group (n=16) animals were intramuscularly injected 2 ml of saline on d 13. Progesterone levels in Implant group on d 16 and 20 were higher than that of in GnRH and Control groups (p<0.05). Pregnancy rates on d 28 were 43.75% (7/16), 50% (8/16) and 18.75% (3/16) in GnRH, Implant and Control groups, respectively, and it was higher in Implant group than that of in control group (p=0.06). In conclusion, progesterone supplementation between d 13 and 20 via ear implant increased the progesterone levels on d 16 and 20, and increased pregnancy rate compared to Control group

Anahtar Kelimeler

GnRH, ear implant, plasma progesterone, pregnancy, embryo transfer, cow

Kaynakça

• Ataman, M. B., Erdem, H., Bülbül, B., Ümütlü, S., Colak, M. (2011). The effect of buserelin injection 12 days after insemination on selected reproductive characteristics in cows. Acta Vet. Brno. 80, 171-177
• Beltman, M. E., Lonergan, P., Diskin, M. G., Roche, J. F., Crowe, M. A. (2009). Effect of progesterone supplementation in the first week post conception on embryo survival in beef heifers. Theriogenology 71, 1173-1179
• Block, J., Drost, M., Monson, R. L., Rutledge, J. J., Rivera, R. M., Paula-Lopes, F. F., Ocon, O. M., Krininger, C. E., Liu, J., Hansen, P. J. (2003). Use of insulin-like growth factor-I during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed, lactating cows. J. Anim. Sci. 81, 1590-1602
• Bogacki, M., Silvia, W. J., Rekawiecki, R., Kotwica, J. (2002). Direct inhibitory effect of progesterone on oxytocin-induced secretion of prostaglandin F(2alpha) from bovine endometrial tissue. Biol. Reprod. 67, 184-188
• Bülbül, B., Kırbaş, M., Köse, M., Dursun, S., Çolak, M. (2009). İneklerde Östrüs Siklusunun Farklı Dönemlerinde Başlatılan Ovsynch Protokolünün Östrüs Senkronizasyonuna Etkileri [Turkish]. Istanbul Univ. Vet. Fak. Derg. 35, 7-17
• Chagas e Silva, J., Diniz, P., Lopes da Costa, L. (2008). Luteotrophic effect, growth and survival of whole versus half embryos and, their relationship with plasma progesterone concentrations of recipient dairy heifers. Anim. Reprod. Sci. 104, 18-27
• Chenault, J. R., Boucher, J. F., Dame, K. J., Meyer, J. A., Wood-Follis, S. L. (2003). Intravaginal progesterone insert to synchronize return to estrus of previously inseminated dairy cows. J. Dairy Sci. 86, 2039-2049
• Doležel, R., Čech, S., Zajic, J., Havliček, V. (2002). Oestrus synchronization by PGF2α and GnRH in intervals according to stage of follicular development at time of initial treatment in cows. Acta Vet. Brno. 71,101- 108
• Ellington, J. E., Foote, R. H., Farrell, P. B., Hasler, J. F., Webb, J., Henderson, W. B., McGrath, A. B. (1991). Pregnancy rates after the use of a gonadotropin releasing hormone agonist in bovine embryo transfer recipients. Theriogenology 36, 1035-1042
• Franco, M., Thompson, P. M., Brad, A. M., Hansen, P. J. (2006). Effectiveness of administration of gonadotropin-releasing hormone at Days 11, 14 or 15 after anticipated ovulation for increasing fertility of lactating dairy cows and non-lactating heifers. Theriogenology 66, 945-954
• Galimberti, A. M., Fonseca, F. A., Araujo, M. C. C., Costa, E. P. d., Freitas, C. d., Guimarães, J. D., Ferreira, A. M. (2001). Taxa de gestação e níveis plasmáticos de progesterona, em receptoras de embrião bovino, tratadas com buserelina após a inovulação [Portuguese]. Rev. Bras. Zootec. 30, 353-359
• Hasler, J. F. (2001). Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology 56, 1401-1415
• Hasler, J. F., Hurtgen, P. J., Jin, Z. Q., Stokes, J. E. (1997). Survival of IVF-derived bovine embryos frozen in glycerol or ethylene glycol. Theriogenology 48, 563-579
• Herman, H.A., Mitchell, J.R., Doak, G.A. (1994). The artificial insemination and embryo transfer of dairy cattle. Interstate Publishers, Illinois
• Karimi, A., Shabankareh, H. K., Moeini, M. M. (2007). The effect of administration of different levels of GnRH on the day 0, 5 and 12 post-insemination on progesterone concentration in dairy heifers. Pak. J. Biol. Sci. 10, 3620-3625
• Long, S. T., Nakao, T., Wakatake, S., Okakoi, M. (2010). Effect of CIDR 12 to 19 days after AI on detection of returning estrus and conception rate in dairy cows. J. Reprod. Dev. 56, 251-255
• Looney, C. R., Nelson, J. S., Schneider, H. J., Forrest, D. W. (2006). Improving fertility in beef cow recipients. Theriogenology 65, 201-209
• Mann, G. E., Fray, M. D., Lamming, G. E. (2006). Effects of time of progesterone supplementation on embryo development and interferon-tau production in the cow. Vet. J. 171, 500-503
• Mann, G. E., Merson, P., Fray, M. D., Lamming, G. E. (2001). Conception rate following progesterone supplementation after second insemination in dairy cows. Vet. J. 162, 161-162
• Mehni, S. B., Shabankareh, H. K., Kazemi-Bonchenari, M., Eghbali, M. (2012). The comparison of treating Holstein dairy cows with progesterone, CIDR and GnRH after insemination on serum progesterone and pregnancy rates. Reprod. Domest. Anim. 47, 131-134
• Purcell, S. H., Beal, W. E., Gray, K. R. (2005). Effect of a CIDR insert and flunixin meglumine, administered at the time of embryo transfer, on pregnancy rate and resynchronization of estrus in beef cattle. Theriogenology 64, 867-878
• Rettmer, I., Stevenson, J. S., Corah, L. R. (1992a). Endocrine responses and ovarian changes in inseminated dairy heifers after an injection of a GnRH agonist 11 to 13 days after estrus. J. Anim. Sci. 70, 508-517
• Rettmer, I., Stevenson, J. S., Corah, L. R. (1992b). Pregnancy rates in beef cattle after administering a GnRH agonist 11 to 14 days after insemination. J. Anim. Sci. 70, 7-12
• Schmitt, E. J., Diaz, T., Barros, C. M., de la Sota, R. L., Drost, M., Fredriksson, E. W. (1996). Differential response of the luteal phase and fertility in cattle following ovulation of the first-wave follicle with human chorionic gonadotropin or an agonist of gonadotropin-releasing hormone. J. Anim. Sci. 74, 1074- 1083
• Smith, A. K., Broadbent, P. J., Dolman, D. F., Grimmer, S. P., Davies, D. A., Dobson, H. (1996). Norgestomet implants, plasma progesterone concentrations and embryo transfer pregnancy rates in cattle. Vet. Rec. 139, 187-191
• Smith, A. K., Grimmer, S. P. (2002). Pregnancy rates for grade 2 embryos following administration of synthetic GnRH at the time of transfer in embryo-recipient cattle. Theriogenology 57, 2083-2091
• Spencer, T. E., Burghardt, R. C., Johnson, G. A., Bazer, F. W. (2004). Conceptus signals for establishment and maintenance of pregnancy. Anim. Reprod. Sci. 82-3, 537-550
• Stevenson, J. S., Phatak, A. P., Rettmer, I., Stewart, R. E. (1993). Postinsemination administration of receptal-follicular dynamics, duration of cycle, hormonal responses, and pregnancy rates. J. Dairy Sci. 76, 2536-2547
• Thatcher, W. W., Staples, C. R., Danet-Desnoyers, G., Oldick, B., Schmitt, E. P. (1994). Embryo health and mortality in sheep and cattle. J. Anim. Sci. 72, 16-30
• Tribulo, R., Nigro, M., Burry, E., Caccia, M., Tribulo, H., Bo, G. A. (1997). Pregnancy rates in recipients receiving CIDR-B devices immediately following embryo transfer. Theriogenology 47, 372
• Tubino, F. A., Laraco, C. (1999). Pregnancy rates in embrayo transfer recipients following RH administration on day 13 of the estrous cycle. Theriogenology 51, 405
• Willard, S., Gandy, S., Bowers, S., Graves, K., Elias, A., Whisnant, C. (2003). The effects of GnRH administration postinsemination on serum concentrations of progesterone and pregnancy rates in dairy cattle exposed to mild summer heat stress. Theriogenology 59, 1799-1810
• Yildiz, H., Kaygguzsuzoglu, E., Kaya, M., Cenesiz, M. (2009). Effect of post-mating GnRH treatmet on serum progesterone, luteinizing hormone levels, duration of estrous cycle and pregnancy rates in cows. Pakistan J. Vet. 29, 110-114