Investigation of the Anti-Cancer Effects of Kitosan Added Red Pine (Pinus Brutia Ten, Pinaceae) Cone Extracts

Yıl 2022, , 16 - 22, 19.03.2023

Serap Özkaya , Beyzanur Balkis , Dide Irmak Özçelik , Cansu Olguner , Ramazan Uludağ , Esra Aydemir

https://doi.org/10.37215/bilar.1030055

Öz

Cancer is a systemic disease that occurs with the accumulation of many mutations in the genome. There
are many different treatment methods applied to cancer patients. During the treatment process, increasing toxicity and drug resistance occur over time and limit the success of the treatment. In order to overcome these limitations in cancer treatment, herbal products and synthetic derivatives can be preferred. At this point, chitosan, which is formed by deacetylating approximately half of the Korean pine (Pinus koraiensis) and long-chain polymer derivative chitin, which has been used in the treatment of many diseases in traditional medicine until today , stands out. In this study, the effects of red pine (Pinus brutia Ten) cone extract on MCF-7, VERO and HeLa cells after 24, 48 and 72 hours of incubation were investigated. The WST-1 cytotoxicity test was analyzed by applying the extracts to the cells at doses of 400, 200, 100, 50 and 25μg/mL. In the experimental results, while chitosan added green cone extract did not cause cytotoxic effects on cells after 24 hours of incubation, the IC50 value for the extract was calculated as 252.3 μg/mL at the end of 48 hours. The calculated IC50 value for the extract after 72 hours is 195.5μg/mL. IC50 values of chitosan added green pinecone extract in HeLa cells at 24, 48 and 72 hours incubation times were calculated as 200.5 μg/mL, 221.9 μg/mL and 352.3 μg/ml, respectively. IC50 values in HeLa cells in 24 and 48 hour incubations were calculated as 262.6 μg/mL and 161.9 μg/mL, respectively , in black pinecone extract with chitosan.

Anahtar Kelimeler

MCF-7, HeLa, Cytotoxicity, Chitosan, Pinecone Extract

Kitosan Katkılı Kızılçam (Pinus Brutia Ten, Pinaceae) Kozalağı Ekstraktlarının Antikanser Etkilerinin Araştırılması

Öz

Kanser, genomdaki birçok mutasyonun birikimi ile ortaya çıkan sistemik bir hastalıktır. Kanser
hastalarında uygulanan birçok farklı tedavi yöntemi bulunmaktadır. Tedavi süreci içerisinde zamanla artan toksisite ve ilaç direnci ortaya çıkmakta ve tedavi başarısını sınırlandırmaktadır. Kanser tedavisindeki bu sınırlandırmaları aşmak için bitkisel ürünler ve sentetik türevleri tercih edilebilmektedir. Bu noktada, geleneksel tıpta günümüze kadar birçok hastalığın tedavisinde kullanılan Kore çamı (Pinus koraiensis) ve uzun zincirli polimer türevi olan kitinin yaklaşık olarak yarısının deasetile edilmesiyle oluşturulan kitosan göze çarpmaktadır . Bu çalışmada kızılçam (Pinus brutia Ten) kozalağı ekstraktının MCF-7, VERO ve HeLa hücreleri üzerinde 24, 48 ve 72 saatlik inkübasyonlar sonundaki etkileri incelenmiştir. Ekstraktlar hücrelere 400, 200, 100, 50 ve 25μg/mL dozlarda uygulanarak maruziyet süreleri sonunda WST-1 sitotoksisite testi analiz edilmiştir. Deney sonuçlarında kitosan katkılı yeşil kozalak ekstraktı 24 saatlik inkübasyon sonunda hücrelerde sitotoksik etki yaratmazken, ekstrakt için 48 saat sonunda IC50 değeri 252.3 μg/mL olarak hesaplanmıştır. 72 saat sonrasında ekstrakt için hesaplanan IC50 değeri 195.5μg/mL ‘dir. Kitosan ilaveli yeşil çam kozalağı ekstraktının HeLa hücrelerinde 24, 48 ve 72 saatlik inkübasyon sürelerinde IC50 değerleri sırasıyla 200.5 μg/mL, 221.9 μg/mL ve 352.3 μg/ml olarak hesaplanmıştır. Kitosan ilaveli siyah çam kozalağı ekstraktında HeLa hücrelerinde 24 ve 48 saatlik inkübasyonlarda IC50 değerleri sırasıyla 262.6 μg/mL ve 161.9 μg/mL olarak hesaplanmıştır .

Anahtar Kelimeler

MCF-7, HeLa, Sitotoksisite, Kitosan, Çam Kozalağı Ekstraktı

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