Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi

Yıl 2020, Cilt: 13 Sayı: 3, 244 - 251, 15.12.2020

Betül Yılmaz Öztürk , Derviş Öztürk

https://doi.org/10.46309/biodicon.2020.764145

Cited By: 3

Öz

Kimyasal reaksiyonlarla sentezlenen nanopartiküllerin çeşitli omurgalılarda ve omurgasızlarda geniş bir toksisite yelpazesi sergilese de antifungal aktivitelerinden yararlanmak üzere büyük miktarlarda üretilmektedir. Klasik kimyasal yöntemlerin dikkat çekici dezavantajları nedeniyle, metalik nanopartiküllerin yeşil sentezi son yıllarda büyük ilgi görmeye başlamıştır. Bu yöntem düşük maliyetli, çevre dostu ve basit yaklaşımları ile klasik kimyasal yöntemlere bir alternatif olarak önerilmektedir. Bu çalışmada amacımız, gümüş nanopartiküllerin hücre dışı sentezinde Tilia rubra DC. ekstraktının uygunluğunu belirlemek ve elde edilen materyali karakterize etmektir. Bunun yanısıra biyosentezlenmiş gümüş nanopartiküllerinin antifungal etkisi değerlendirilmiştir. T. rubra ekstraktı, gümüş nanopartiküllerin biyosentezi için ilk kez çalışılmış olup UV-Vis spektrofotometresinde 427 nm'de yüzey plazmon rezonansı oluşturdu. Ayrıca geçirimli elektron mikroskop (TEM) görüntüleri nanopartiküllerin küresel morfolojiye sahip olduğunu gösterdi. TEM görüntülerine göre nanopartiküllerin boyutlarının 5-15 nm arasında olduğu bulundu. X-ışını kırınımı (XRD) analizi ise partiküllerin yüz merkezli kübik geometriye sahip kristal yapıda olduğunu belirledi. Gümüş iyonlarının biyo-indirgenmesinden sorumlu olası biyomolekülleri tanımlamak için Fourier dönüşümlü kızılötesi spektroskopisi (FTIR) analizi yapıldı. Biyosentezlenmiş gümüş nanopartiküllerin patojen bir maya olan Candida albicans üzerindeki antifungal etkisi agar difüzyon metodu ile incelendi ve sonuç olarak sentezlenen gümüş nanopartiküllerinin, Candida albicans üzerinde önemli inhibe edici etki gösterdi. Biyosentezlenmiş gümüş nanopartiküllerin çevre dostu sentez prosedürü gelecekte endüstriyel ve biyomedikal uygulamalar için kullanılma potansiyeline sahip olduğunu desteklemektedir.

Anahtar Kelimeler

Yeşil sentez, Tilia rubra, gümüş nanopartikül, karakterizasyon, antifungal aktivite

Teşekkür

Bu çalışma Eskişehir Osmangazi Üniversitesi Merkez Araştırma Laboratuvarı Uygulama ve Araştırma Merkezi'nde (ARUM) çalışılmıştır.

Kaynakça

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