The protective effects of Selenium and Boron against Cyclophosphamide-induced bone marrow and blood toxicity: An in vivo study

Year 2022, Volume: 15 Issue: 2, 256 - 264, 15.08.2022

Adnan Ayhancı , Nilüfer Lafçı , Ahmet Musmul , Fatma Gür , Canan Vejselova Sezer , İlknur Kulcanay Şahin , Bahri Gür , Namık Bilici , Suzan Onur , Mustafa Cengiz

https://doi.org/10.46309/biodicon.2022.1124346

Cited By: 2

Abstract

Thanks to their antioxidant, anti-apoptotic, anti-lipid peroxidative, and immune-boosting properties, Boron (B) and Selenium (Se) are essential trace elements for the human body. This study aims to compare the myeloid protective potentials of Se and B in Cyclophosphamide (CP)-induced bone-marrow and haematological toxicity in experimental rats considering that the myelotoxic property of this anti-cancer drug limits its use. We hypothesized that selenium has a better protective effect than boron in preventing the toxic effects of CP on bone marrow and blood cells. 1.5 mg/kg of Se and 20 mg/kg of B, which are the most frequently used optimal doses of these trace elements, were given to the animals intraperitoneally throughout the experiment. 200 mg/kg of CP was administered only on the 4th day. The animals were sacrificed to take the blood and bone marrow samples to be stored for hematological evaluations. The CP administration significantly decreased leukocyte (WBC), thrombocyte (PLT), erythrocytes (RBC), and bone marrow nucleated cell counts. On the other hand, they increased in significant amounts in the groups given Se and B along with CP when compared to those given only CP. However, Se proved to be more protective than B in preventing CP-induced bone marrow and hematologic toxicity despite not achieving statistical significance. It was, therefore, concluded that the doses used in this experiment were successful in protecting against CP-induced damage to the bone marrow and CP-related hematological toxicity.

Keywords

Cyclophosphamide, Selenium, Boron, Bone marrow, Hematologic toxicity

Selenyum ve Bor'un Siklofosfamid kaynaklı kemik iliği ve kan toksisitesine karşı koruyucu etkileri: Bir in vivo çalışma

Abstract

Bor (B) ve Selenyum (Se), antioksidan, anti-apoptotik, anti-lipid peroksidatif ve bağışıklık güçlendirici özellikleri sayesinde insan vücudu için gerekli eser elementlerdir. Bu çalışma, Se ve B'nin Siklofosfamid (SFD) ile indüklenen kemik iliğinde miyeloid koruyucu potansiyellerini ve bu anti-kanser ilacın miyelotoksik özelliğinin kullanımını sınırladığını göz önünde bulundurarak deneysel sıçanlarda hematolojik toksisiteyi karşılaştırmayı amaçlamaktadır. SFD'nin kemik iliği ve kan hücreleri üzerindeki toksik etkilerini önlemede selenyumun bordan daha iyi bir koruyucu etkiye sahip olduğunu varsaydık. Bu eser elementlerin en sık kullanılan optimal dozları olan 1.5 mg/kg Se ve 20 mg/kg B hayvanlara deney boyunca intraperitoneal olarak verildi. Sadece 4. günde 200 mg/kg SFD uygulandı. Hayvanlar sakrifiye edilerek hematolojik değerlendirmeler için kan ve kemik iliği örnekleri alındı. SFD uygulaması, lökosit (WBC), trombosit (PLT), eritrositler (RBC) ve kemik iliği çekirdekli hücre sayılarını önemli ölçüde azalttı. Öte yandan, SFD ile birlikte Se ve B verilen gruplarda, sadece SFD verilenlere göre önemli miktarlarda arttı. Ancak Se'nin, istatistiksel anlamlılık elde edememesine rağmen, SFD'nin neden olduğu kemik iliği ve hematolojik toksisiteyi önlemede B'den daha koruyucu olduğu kanıtlanmıştır. Bu nedenle, bu deneyde kullanılan dozların, kemik iliğinde SFD'nin neden olduğu hasara ve SFD'e bağlı hematolojik toksisiteye karşı korumada başarılı olduğu sonucuna varıldı.

Keywords

Siklofosfamid, Selenyum, Bor, Kemik iliği, Hematolojik Toksisite

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