Survey for Grapevine leafroll viruses (GLRaVs) in Malatya region

Yıl 2018, Cilt: 58 Sayı: 4, 215 - 220, 29.12.2018

Hatice Diğdem Oksal , Songül Aydın , Hikmet Murat Sipahioğlu

https://doi.org/10.16955/bitkorb.430912

Cited By: 1

Öz

Grapevine (Vitis vinifera L.) is one of the most important fruit crops in Turkey
and viral diseases are one of the main problems of grapevine production. They
decrease yield quantity and quality of crops as well as cause death of the plants
in an interaction with other agents. Leafroll disease of grapevine, caused by a
complex of viruses, is considered a serious threat to production across many
grapevine growing regions in Turkey. This study was conducted to determine
the current status of Grapevine leafroll-associated viruses (GLRaVs) in Malatya
province. Grapevine plantations in Malatya province were surveyed for the
presence of Grapevine leafroll-associated viruses (GLRaVs) -1, -2, -3, -4 and -6.
Purposive sampling was done in vineyards of Arapgir, Darende and Battalgazi
districts, which are the primary grapevine producing areas of Malatya during
2016-2017 growing seasons. A total of 241 samples were collected and tested by
DAS-ELISA. Samples giving two fold of absorbance values (405 nm) of negative
control were evaluated as positive. According to the results, 131 samples were
found to be infected with GLRaV-1, whereas 48 samples with GLRaV-3 and -4
samples with GLRaV-4. None of the samples were reacted as positive against
GLRaV-2 and GLRaV-6. Forty five samples were found to be infected with mix
infection of GLRaV-1 and -3, and three samples were found to be infected with
GLRaV-1 and -4 strain 6.

Anahtar Kelimeler

Survey, grapevine, Grapevine leafroll associated viruses, Malatya

Malatya bölgesi bağlarında Asma yaprak kıvrılma virüsleri (GLRaVs)’nin sürveyi

Öz

Asma (Vitis
vinifera L.) ülkemizde yetiştiriciliği yapılan en önemli meyvelerden birisi
olup üretimini sınırlayan faktörlerin başında virüs hastalıklar gelmektedir.
Virüs hastalıkları üzüm üretimini kalite ve kantite bakımından azaltmanın yanı
sıra diğer hastalık etmenleri ile beraber bulunmaları durumunda asmanın
kurumasına kadar ulaşabilen ciddi sonuçlar doğurabilmektedir. Bir virüs kompleksinin
neden olduğu Asma yaprak kıvrılma hastalığı ülkemiz asma
yetiştiriciliği üzerinde önemli bir tehdittir. Bu çalışma, Malatya ilinde
yetiştirilen asmaları etkileyen Asma yaprak kıvırcıklık virüsleri (Grapevine leafroll associated virus, GLRaV)’ini tespit etmek
amacı ile yürütülmüştür. Bu amaçla Malatya ilindeki asma bahçeleri gezilerek etmenin
1, 2, 3, 4 ve 6 nolu ırkları araştırılmıştır. 2016-2017 yıllarında Arapgir,
Darende ve Battalgazi ilçelerini kapsayan survey çalışmalarında güdümlü
örneklemeler yapılarak asma numuneleri toplanmıştır. Toplanan 241 örneğin
tamamı DAS-ELSA metodu ile testlenmiştir. Testlerde negatif örneğin iki katı ve
yukarısında absorbans (405 nm) değeri veren numuneler infekteli kabul
edilmiştir. Yürütülen test sonuçlarına göre toplam 131 numunenin GLRaV 1 ile,
48 numunenin GLRaV 3 ile ve 4 örneğin ise GLRaV 4 ile infekteli olduğu tespit
edilmiştir. Örneklerin hiçbirinde GLRaV 2 ve GLRaV 6 infeksiyonuna
rastlanmamıştır. Testlenen 45 numunenin GLRaV 1 ve 3 ile, üç numunenin ise GLRaV
1 ve 4 ile karışık infeksiyon halinde bulunduğu tespit edilmiştir. 

Anahtar Kelimeler

Survey, grapevine, Grapevine leafroll associated viruses, Malatya

Kaynakça

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