Entomopatojen nematodların üç uygulama tekniği ile patates böceği [Leptinotarsa decemlineata (Say), (Coleoptera: Chrysomelidae)] üzerinde etkilerinin sera koşullarında araştırılması

Yıl 2022, Cilt: 62 Sayı: 4, 14 - 21, 31.12.2022

Niyazi Güleç , İlker Kepenekçi

https://doi.org/10.16955/bitkorb.1150809

Öz

Patates üretimini azaltan zararlı organizmalardan biri de patates böceği [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] (PB)’dir. Patates bitkisinin her dönemine zarar veren polifag bir zararlıdır. Dünya’da entomopatojen nematodların (EPN) birçok zararlı grubuna karşı etkinliği laboratuvar ve tarla/bahçe çalışmaları ile ortaya konulmuş olmasına karşın ülkemizde PB ile ilgili çok az çalışma yapılmıştır. Yapılan bu çalışma kapsamında PB ile mücadelede EPN’lerin kullanılması amaçlanmıştır. Çalışmanın ana hedefi, laboratuvar stoklarımızda mevcut olan EPN [Steinernema feltiae (izolat 09-31) and Heterorhabditis bacteriophora (izolat 09-43)]’lerin toprak, yeşil aksam ve kadavra uygulamaları şeklinde sera-saksı denemeleri oluşturmuştur. Türkiye’de ilk defa bu zararlı grubuna karşı sera-saksı çalışmaları yürütülmüştür. Ayrıca dünyada EPN’lerin sulu konsantrasyonlarının zararlılara karşı kullanımı ile ilgili çok çalışma bulunmaktadır. Son yıllarda sulu konsantrasyonların yerine kadavra içinde EPN’lerin kullanılması çalışmaları başlamıştır. PB’ye karşı bu uygulama (kadavra uygulamaları) ilk çalışma niteliğindedir. Araştırma sonucu elde edilen sonuçlara göre EPN’lerin toprağa yapılan uygulamalarda en yüksek ölüm oranı S. feltiae (65.23±4.45 ve 77.33±2.59)’da elde edilmiştir. Diğer uygulamalar (yeşil aksam ve kadavra uygulamaları)’da etkisinin düşük düzeyde olduğu görülmektedir. Deneme sonuçlarında, kadavra uygulamalarında ölüm oranı %40’ı geçmemiş ve en yüksek ölüm oranı H. bacteriophora’da %37.40±8,88 olarak tespit edilmiştir. Yeşil aksam uygulamalarında ise ölüm oranı %30’u geçmemiş ve en yük ölüm oranı H. bacteriophora’da 29.14±6.09 olarak tespit edilmiştir. EPN sera-saksı deneme sonuçlarına göre S. feltiae (izolat 09-31)’nın toprak uygulamalarının arazi denemeleri kapsamına alınması uygun olacaktır.

Anahtar Kelimeler

entomopatojen nematodlar, patates böceği, biyolojik mücadele.

Research on the efficacy of three application techniques of entomopathogenic nematodes against the Colorado potato beetle [Leptinotarsa decemlineata (Say), (Coleoptera: Chrysomelidae)] under greenhouse conditions

Öz

Colorado Potato Beetle [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] (CPB) is one of the most destructive pests of potatoes. CPB is a polyphagous pest that damages every stage of the potato. Although entomopathogenic nematodes (EPNs) in the world have been demonstrated by many laboratories and field/garden studies activity against many harmful groups, very few studies have been conducted on CPB in our country (Türkiye). In the scope of work intended to be used EPNs against CPB. The main objective of the study is to reveal greenhouse-pot applications (soil, green limbs, and cadaver applications) of EPN [Steinernema feltiae (isolate 09-31) and Heterorhabditis bacteriophora (isolate 09-43)], which are present in our laboratories in our stocks. For the first time in Türkiye, greenhouse-pot applications studies have been carried out against this harmful group. There is also a lot of work in the world about the use of aqueous concentrations of EPNs against harmful effects. In recent years, efforts have been made to use EPNs in cadavers instead of aqueous concentrates. This application (cadaver applications) against CPB is the first working. The results revealed that EPNs performed better in soil applications, and the highest mortality rate was obtained from S. feltiae (65.23±4.45 and 77.33±2.59). Other applications (green limbs and cadaver applications) are seen to have a low level of efficacy. In trials, the mortality rate in cadaver applications did not exceed 40%, and the lowest mortality rate in H.bacteriophora was 37,40±8,88%. In the case of green devices, the mortality rate did not exceed 30%, and the highest mortality rate was 29.14±6.09 in H.bacteriophora. According to EPN greenhouse-pot experiments results, soil applications of S. feltiae (isolate 09-31) should be included within the scope of field trials.

Anahtar Kelimeler

entomopathogenic nematodes, Colorado potato beetle, Leptinotarsa decemlineata, biological control, entomopatojen nematodlar, patates böceği, biyolojik mücadele.

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