Mısır fidelerinin büyüme ve gelişmesi üzerine progesteronun doğrudan etkisi

Yıl 2019, Cilt: 8 Sayı: 2, 396 - 405, 28.06.2019

Hülya Türk

https://doi.org/10.17798/bitlisfen.473607

Cited By: 1

Öz

Memeli cinsiyet
hormonları arasında yer alan ve bitkilerde de doğal olarak bulunan progesteron,
çimlenmeden çiçeklenmeye kadar tüm bitki gelişim safhaları üzerine önemli
uyarıcı etkilere sahiptir. Ancak bu hormonun etki mekanizması halen tam olarak
aydınlatılamadığı gibi, sebep olduğu etkilerin de direkt olarak kendinden mi
kaynaklandığı yoksa farklı yolaklar üzerinden dolaylı olarak mı gerçekleştiği
halen bilinmemektedir. Bu amaçla, mevcut araştırmada progesteron reseptör
antagonisti olan mifepriston kullanılmak suretiyle, progesteronun etkisinin
doğrudan mı yoksa dolaylı mı olduğu belirlenmeye çalışılmıştır. 9 günlük mısır
fideleri 4 gruba ayrılarak yapraklarına saf su (kontrol grubu), progesteron
(0.1 µmol.L^-1), mifepriston (5.8×10^-5
mmol.L^-1) ve progesteron + mifepriston) solüsyonları
püskürtülmüş ve tüm gruplar üç gün sonra hasat edilerek çeşitli analizler
yapılmıştır. Progesteron uygulaması bitkilerin kök ve yaprak uzunluları ile
total klorofil ve kuru madde içeriklerinde kontrol bitkilerine kıyasla önemli
artışlara sebep olurken, mifepriston uygulanan bitkilerde bu parametrelerin
tamamında belirgin düşüşler kaydedildi. Progesteron ile birlikte mifepriston
uygulanan bitkilerde ise kontrol bitkilerine kıyasla istatistiki olarak önemli
bir değişim meydana gelmedi. Tek başına mifepriston uygulaması ise bu
parametrelerde belirgin bir inhibisyona neden oldu. Tüm bu bulgular,
mifepriston uygulamasının progesteronun bağlanmasını önlemek suretiyle
progesteron bağlantılı metabolik faaliyetlerin gerçekleşmesine ket vurarak
mısır fidelerinin büyüme ve gelişmesini inhibe ettiğini ve böylelikle
progesteronun bitki metabolizması üzerine doğrudan etkiye sahip olduğunu açıkça
ortaya koymaktadır.

Anahtar Kelimeler

progesteron, mifepriston, mısır, bitki büyümesi

Kaynakça

• 1. Janeczko A., Barna B., Gullner G., Skoczowski A., Dubert F. 2005. Specific Physiological Activity of 24-Epibrassinolide Injected into Apoplast of Spring Rape Tissues Prior to Cold Treatment, Acta Physiologiae Plantarum, 27: 52-52.
• 2. Janeczko A. 2012. The Presence and Activity of Progesterone in the Plant Kingdom, Steroids, 77: 169-173.
• 3. Janeczko A., Oklestkova J., Novak O., Sniegowska-Swierk K., Snaczke Z., Pociecha E. 2015. Disturbances in Production of Progesterone and Their Implications in Plant Studies, Steroids, 96: 153-163.
• 4. Erdal S., Dumlupinar R. 2011. Mammalian Sex Hormones Stimulate Antioxidant System and Enhance Growth of Chickpea Plants, Acta Physiologia Plantarum, 33: 1011-1017.
• 5. Erdal S. 2012. Alleviation of Salt Stress in Wheat Seedlings by Mammalian Sex Hormones, Journal of the Science of Food and Agriculture, 92: 1411-1416.
• 6. Erdal S. 2012. Exogenous Mammalian Sex Hormones Mitigate Inhibition in Growth by Enhancing Antioxidant Activity and Synthesis Reactions in Germinating Maize Seeds Under Salt Stress, Journal of the Science of Food and Agriculture, 92: 839-843.
• 7. Chaoui A., El Ferjani E. 2014. Heavy Metal-induced Oxidative Damage is Reduced by beta-Estradiol Application in Lentil Seedlings, Plant Growth Regulation, 74: 1-9.
• 8. Janeczko A., Oklest'kova J., Siwek A., Dziurka M., Pociecha E., Kocurek M., Novak O. 2013. Endogenous Progesterone and its Cellular Binding Sites in Wheat Exposed to Drought Stress, Journal of Steroid Biochemistry, 138: 384-394.
• 9. Hu C., Hermann G., Pen-Mouratov S., Shore L., Steinberger Y. 2011. Mammalian Steroid Hormones can Reduce Abundance and Affect the Sex Ratio in a Soil Nematode Community, Agriculture, Ecosystems & Environment, 142: 275-279.
• 10. Agarwal M.K. 1993. Receptors for Mammalian Steroid-Hormones in Microbes and Plants, Febs Letters, 322: 207-210.
• 11. Dumlupinar R., Genisel M., Erdal S., Korkut T., Taspinar M.S., Taskin M. 2011. Effects of Progesterone, beta-Estradiol, and Androsterone on the Changes of Inorganic Element Content in Barley Leaves, Biological Trace Element Research, 143: 1740-1745.
• 12. Erdal S., Genisel M. 2016. The Property of Progesterone to Mitigate Cold Stress in Maize is Linked to a Modulation of the Mitochondrial Respiratory Pathway, Theoretical and Experimental Plant Physiology, 28: 385-393.
• 13. Erdal S., Genisel M., Turk H., Gorcek Z. 2012. Effects of Progesterone Application on Antioxidant Enzyme Activities and K+/Na+ Ratio in Bean Seeds Exposed to Salt Stress, Toxicology and Industrial Health, 28: 942-946.
• 14. Simersky R., Novak O., Morris D.A., Pouzar V., Strnad M. 2009. Identification and Quantification of Several Mammalian Steroid Hormones in Plants by UPLC-MS/MS, Journal of Plant Growth Regulation, 28: 125-136.
• 15. Su X.Y., Wu S., Yang L., Xue R.L., Li H., Wang Y.X., Zhao H.J. 2014. Exogenous Progesterone Alleviates Heat and High Light Stress-induced Inactivation of Photosystem II in Wheat by Enhancing Antioxidant Defense and D1 Protein Stability, Plant Growth Regulation, 74: 311-318.
• 16. Jumrani K., Bhatia V.S. 2018. Impact of Combined Stress of High Temperature and Water Deficit on Growth and Seed Yield of Soybean, Physiology and Molecular Biology of Plants, 24: 37-50.
• 17. Barrs H.D., Weatherley P.E. 1962. A Re-Examination of the Relative Turgidity Techniques for Estimating Water Deficits in Leaves, Australian Journal of Biological Sciences, 15: 413-428.
• 18. Serdar, U., Demirsoy, H. 2006. Non-destructive Leaf Area Estimation in Chestnut, Scientia Horticulturae , 108 (2): 227-230.
• 19. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. 1985. Measurement of Protein Using Bicinchoninic Acid, Analytical Biochemistry, 150: 76-85.
• 20. Dische Z. 1947. New Characteristic Color Reactions of Carbohydrates with SH Compounds in H2SO4, Federation Proceedings, 6: 248.
• 21. Witham F.H., Blaydes B.F., Devlin R.M. 1971. Experiments in Plant Physiology, Van Nostrand Reinhold, New York, USA,167-200.
• 22. Sharkey T.D., Savitch L.V., Butz N.D. 1991. Photometric-Method for Routine Determination of Kcat and Carbamylation of Rubisco, Photosynthesis Research, 28: 41-48.
• 23. Janeczko A. 2000. Influence of Selected Steroids on Plant Physiological Processes – Especially Flowering Induction. Agricultural University, Phd thesis. Krakow, Poland.
• 24. Erdal S., Dumlupinar R. 2010. Progesterone and beta-Estradiol Stimulate Seed Germination in Chickpea by Causing Important Changes in Biochemical Parameters, Zeitschrift für Naturforschung C, 65: 239-244.
• 25. Erdal S., Dumlupinar R., Cakmak T., Genisel M. 2010. Mammalian Sex Hormones Influence Germination Velocity and Enzyme Activities in Germinating Maize Seeds, Fresenius Environmental Bulletin, 19: 1458-1465.
• 26. Erdal S. 2009. Effects of Mammalian Sex Hormones on Antioxidant Enzyme Activities, H2O2 Content and Lipid Peroxidation in Germinating Bean Seeds, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 40: 79-85.
• 27. Yang Y., Wei X., Lu J., You J., Wang W., Shi R. 2010. Lead-induced Phytotoxicity Mechanism Involved in Seed Germination and Seedling Growth of Wheat (Triticum aestivum L.), Ecotoxicology and Environmental Safety, 73: 1982-1987.
• 28. Genisel M., Turk H., Erdal S., Demir Y., Genc E., Terzi I. 2015. Ameliorative Role of beta-Estradiol Against Lead-induced Oxidative Stress and Genotoxic Damage in Germinating Wheat Seedlings, Turkish Journal of Botany, 39: 1052-1060.
• 29. Kopcewicz J. 1969. Influence of Estrone on Growth and Endogenous Giberellins Content in Dwarf Pea, Bulletin de L'Academie Polonaise des Sciences,17 (11): 727-731.
• 30. Dogra R., Thukral A.K. 1994. Effect of Steroid and Plant Hormones on Growth of Maize cv. Canga 5, Crops Research, 2: 42-47.
• 31. Genisel M., Turk H., Erdal S. 2013. Exogenous Progesterone Application Protects Chickpea Seedlings Against Chilling-induced Oxidative Stress, Acta Physiology Plantarum, 35: 241-251.
• 32. Dogra R., Thukral A.K. 1991. Effect of Steroids on Some Growth and Biochemical Parameters of Triticum aestivum L. During Germination, Crops Research, 8: 611-620.
• 33. Kaur G., Kumar S., Thakur P., Malik J.A., Bhandhari K., Sharma K.D., Nayyar H. 2011. Involvement of Proline in Response of Chickpea (Cicer arietinum L.) to Chilling Stress at Reproductive Stage, Scientia Horticulturae, 128: 174-181.
• 34. Dogra R., Thukral A.K. 1989. Effect of Steroid and Plant Hormones on Growth of Maize cv. Ganga 5, Crops Research, 2: 42-48.
• 35. Omalley B.W., Means A.R.1974. Female Steroid-Hormones and Target-Cell Nuclei, Science, 183: 610-620.
• 36. Gioelli F. 1942. Die Wirkung des Follikelhormons (Ostradiol, Dihydrofollikulin)auf Kulturen Pflanzlicher Gewebe in vitro, Archives of Biological Sciences, 28: 311.
• 37. Swamy K.N., Rao S.S.R. 2009. Effect of 24-Epibrassinolide on Growth, Photosynthesis, and Essential Oil Content of Pelargonium graveolens (L.) Herit, Russian Journal of Plant Physiology, 56: 616-620.
• 38. Yang X.H., Xu Z.H., Xue H.W. 2005. Arabidopsis Membrane Steroid Binding Protein 1 is Involved in Inhibition of Cell Elongation, Plant Cell, 17: 116-131.
• 39. Mori D., Ogino N., Yonezawa T. 2011. Kawaminami M., Kurusu S., Anti-ovulatory Effects of RU486 and Trilostane Involve Impaired Cyclooxygenase-2 Expression and Mitotic Activity of Follicular Granulosa Cells in Rats, Prostaglandins Other Lipid Mediators, 94: 118-123.
• 40. Rung E., Friberg P.A., Shao R., Larsson D.G., Nielsen E., Svensson P.A., Carlsson B., Carlsson L.M., Billig H. 2005. Progesterone-receptor Antagonists and Statins Decrease de novo Cholesterol Synthesis and Increase Apoptosis in Rat and Human Periovulatory Granulosa Cells in vitro, Biology of Reproduction, 72: 538-545.
• 41. Yokota T., Nomura T., Nakayama M. 1997. Identification of Brassinosteroids that Appear to be Derived from Campesterol and Cholesterol in Tomato Shoots, Plant and Cell Physiolology, 38: 1291-1294.