Synthesis of hybrid nanoflowers using the purified bitter gourd (Momordica charantia Descourt.) peroxidase and its usability of direct blue 1 decolorization

Yıl 2020, Cilt: 9 Sayı: 2, 573 - 583, 15.06.2020

Cevahir Altınkaynak , Ayşe Baldemir , Nalan Özdemir , Vedat Yılmaz İsmail Öçsoy

https://doi.org/10.17798/bitlisfen.603452

Cited By: 3

Öz

Peroxidase enzymes are purified from different plant
sources are used efficiently for the removal of dyes in industrial wastes. The
fruit of bitter gourd (Momordica
charantia), an inexpensive and easily accessible vegetable, is an important
source of peroxidase. In this study, total protein content was found to be
0.485 mg/mL and peroxidase activity was found to be 2360.9 EU/mg as a result of
50% protein precipitation made from green bitter gourd. However; total protein
amount was 0.232 mg/mL and free peroxidase activity was determined as 7719.30
EU/mg as a result of 60% protein precipitation made from ripe bitter gourd.
Peroxidase enzymes which were purified from bitter gourd in different growth
stages under optimum conditions showed higher enzymatic activity compared to
free forms when immobilized via enzyme-inorganic hybrid nanoflower synthesis
method. The highest peroxidase activity was seen in mature fruit and hybrid
nanoflower form (19661, 6 EU/mg). In addition, the usability of hybrid
nanoflowers was investigated compared to the free purified peroxidase for
removal of Direct Blue 1 dye widely used in textile industry. It was determined
that hybrid nanoflower form synthesized especially by using ripe bitter gourd
peroxidase had more dye removal.

Anahtar Kelimeler

Bitter Gourd Peroxidase, Hybrid Nanoflower

Kudret Narı (Momordica charantia Descourt.) Meyvesinden Saflaştırılan Peroksidaz Enzimi Kullanılarak Hibrit Nano Çiçekler Sentezlenmesi ve Direct blue 1 Gideriminde Kullanılabilirlikleri

Öz

Farklı bitkisel kaynaklardan saflaştırılan peroksidaz
enzimleri verimli bir şekilde endüstriyel atıklarda yer alan boyar maddelerin
gideriminde kullanılmaktadır. Ucuz ve kolay ulaşılabilir bitkisel bir kaynak
olan kudret narı (Momordica charantia)
meyvesi önemli bir peroksidaz kaynağıdır. Bu çalışmada ham kudret narı
meyvesinden yapılan %50 oranında protein çöktürmesi sonucu toplam protein
miktarı 0,485 mg/mL bulunurken peroksidaz aktivitesi 2360,9 EU/mg olarak tespit
edilmiştir. Bunun yanında olgun kudret narı meyvesinden yapılan %60 oranında
protein çöktürmesi sonucu ise toplam protein miktarı 0,232 mg/mL iken serbest
peroksidaz aktivitesi 7719,30 EU/mg olarak tespit edilmiştir. Farklı büyüme
safhalarında yer alan meyvelerden optimum koşullarda saflaştırılmış peroksidaz
enzimleri enzim-inorganik hibrit nano çiçek sentez yöntemi ile immobilize
edildiğinde serbest formlarına göre daha yüksek enzimatik aktivite sergilemişlerdir.
En yüksek peroksidaz aktivitesi olgun meyvede ve hibrit nano çiçek formunda
(19661,6 EU/mg) görülmüştür. Ayrıca çalışmada tekstil endüstrisinde yaygın
olarak kullanılan Direct Blue 1 boyasının giderimi için hibrit nano çiçeklerin
serbest peroksidaz enzimleri ile karşılaştırmalı olarak kullanılabilirlikleri
araştırılmıştır. Özellikle ham meyve peroksidazı kullanılarak sentezlenen
hibrit nano çiçek formunun daha fazla boya giderimi yaptığı tespit edilmiştir.

Anahtar Kelimeler

Kudret Narı Peroksidaz, Hibrit Nano Çiçek

Teşekkür

Ham ve olgunlaşmış kudret narı (M. charantia) temini için Erciyes Üniversitesi Seyrani Ziraat Fakültesi Prof. Dr. Öğretim Üyesi Halit Yetişir’e teşekkür ederiz.

Kaynakça

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