Partial Purification of Catalase Enzyme from Muscle Tissue of Dusky Spinefoot (Siganus luridus)

Yıl 2023, Cilt: 6 Sayı: 2, 140 - 142, 01.03.2023

Büşra Çevik , Sibel Bahadır , Deniz Ekinci

https://doi.org/10.47115/bsagriculture.1213728

Öz

Catalase, one of the antioxidant enzymes, decomposes hydrogen peroxide into water and oxygen. From the discovery of catalase, several studies have been carried out to reveal its importance in health, food and cosmetics industries etc., and these studies are still ongoing. In this study, catalase enzyme was partially purified from muscle tissue of Dusky spinefoot (Siganus luridus). Purification procedure consisted of homogenate preparation, ammonium sulfate precipitation and dialysis. The enzyme was precipitated in the range of 40-60 % Ammonium Sulfate concentration. The optimum buffer was determined as 200 mM phosphate buffer, optimum pH 8.0 and optimum substrate concentration 24mM for H₂ and O₂, respectively.

Anahtar Kelimeler

Purification, Catalase, Characterization, Enzyme

Kaynakça

• Akar Ç. 2015. Purufication of catalase from the walnut (Juglans regia). MSc Thesis, Çukurova University, Institute of Science, Department of Chemistry, Adana, Türkiye, pp: 62.
• Castriota L, Andaloro F. 2008. First record of the lessepsian fish Siganus luridus (Osteichthyes: Siganidae) in the Tyrrhenian Sea. Mar Biodivers Rec, 1: 1.
• Gelen V, Sengul E, Yıldırım S, Senturk E, Tekin S, Kukurt A. 2021. The protective effects of hesperidin and curcumin on 5-fluorouracil-induced nephrotoxicity in mice. Environ Sci Pollut Res Int, 28: 47046-47055.
• Güngör Ö. 2015. Purification of catalase from celery (Apium graveolens). MSc Thesis, Çukurova University, Institute of Science, Department of Chemistry, Adana, Türkiye, pp: 60.
• Jones GL, Masters C J. 1976. On the comparative characteristics of mammalian catalases. Comp Biochem Phys B, 55: 511-518.
• Lanir A, Schejter A. 1975. On the sixth coordination position of beef liver catalase, FEBS Letters, 55: 254-256.
• Masters C, Holmes R. 1977. Peroxisomes: new aspects of cell physiology and biochemistry, Phys Rev, 57: 816-882.
• Nandi A, Yan LJ, Jana CK, Das N. 2019. Role of catalase in oxidative stress-and age-associated degenerative diseases. Oxidat Medic Cell Longevity, 2019: 9613090.
• Ustundag H, Yildirim S, Senturk E, Aliyev E, Yildirim A. 2021. Effect of exercise on oxidant and antioxidant systems in rat kidney tissue with hyperthyroidism. J Med Phys Biophys, 70: 46-53.
• Shinda A, Ganu J, Naik P. 2012. Effect of free radicals & antioxidants on oxidative stress: A review. J Dent Allied Sci, 1(2): 63- 66.
• Sökmen BB, Ahıskalı A. 2017. Purification and characterization of catalase enzyme from fiery milkcap mushrooms (Lactarius pyragalus) growing in Giresun region. Karadeniz Fen Bil Derg, 7: 53-65.