ACTN3 RS1815739, PPARA RS4253778 VE IL6 RS1800795 GEN POLİMORFİZMLERİNİN VOLEYBOLCULARDA AEROBİK DAYANIKLILIK ÜZERİNE ETKİLERİNİN İNCELENMESİ

Yıl 2025, Cilt: 19 Sayı: 1, 17 - 27, 27.03.2025

Mehmet Onur Sever , İbrahim Can , Serdar Bayrakdaroğlu , Sadi Ön , Hüseyin Özkamçı , Gürkan Diker , Mehmet Söyler , Raif Zileli , Özlem Özge Yılmaz , Tolga Polat , Beste Tacal Aslan , Korkut Ulucan

https://doi.org/10.61962/bsd.1540001

Öz

Bu çalışmada voleybolcularda aerobik dayanıklılık ile ACTN3 rs1815739, PPARA rs4253778 ve IL-6 rs1800795 polimorfizmlerini karşılaştırmayı amaçladık. Çalışmaya, 22 voleybol sporcusu (yaş: 21.59 ± 2.70 yıl; boy: 1.87 ± 7.92 m; vücut ağırlığı: 80.99 ± 8.14 kg; vücut yağı: 12.05 ± 5.78%) gönüllü olarak katıldı. Bukkal epitel hücrelerinden DNA ekstraksiyonu ticari bir kit ile sağlandı. Genotipleme için Real-Time PCR yapıldı. Test sonuçlarının istatistiksel analizi SPSS 23 (SPSS Inc., Chicago, IL, ABD) paket programı kullanılarak yapıldı. Varyans homojenliği Levenne testi ile analiz edildi ve normal dağılım Shapiro-Wilk testi ile analiz edildi. Tüm parametreleri analiz etmek için One Way ANOVA Testi kullanıldı. Anlamlılık p<0,05'te belirlendi. ACTN3 rs1815739, PPARA rs4253778 ve IL6 rs1800795 polimorfizmleri ile aerobik performans parametreleri arasında istatistiksel olarak anlamlı bir fark tespit edilmedi (p>0,05). Bulgularda, Kohortumuzda ilgili polimorfizmler ile aerobik performans parametreleri arasında istatistiksel olarak anlamlı bir fark tespit edilmedi. Sonuç olarak, ACTN3 rs1815739, PPARA rs4253778 ve IL6 rs1800795 genleri voleybol oyuncularında aerobik dayanıklılıkta herhangi bir fark yaratmadı. Sonuçlarımızın daha ileri çalışmalarla doğrulanması gerekiyor.

Anahtar Kelimeler

Sporcu, güç, polimorfizm, voleybol

Proje Numarası

GÜBAP2902-21.A0310.02.01

INVESTIGATION OF THE EFFECTS OF ACTN3 RS1815739, PPARA RS4253778 AND IL6 RS1800795 GENE POLYMORPHISM ON AEROBIC ENDURANCE IN VOLLEYBALL PLAYERS

Öz

In this study, we aimed to compare ACTN3 rs1815739, PPARA rs4253778 and IL-6 rs1800795 polymorphisms with aerobic endurance in volleyball players. Twenty-two volleyball players (age: 21.59 ± 2.70 years; height: 1.87 ± 7.92 m; body weight: 80.99 ± 8.14 kg; body fat: 12.05 ± 5.78%) participated in the study voluntarily. DNA extraction from buccal epithelial cells was provided by a commercial kit. Real-Time PCR was performed for genotyping. Statistical analysis of the test results was performed using SPSS 23 (SPSS Inc., Chicago, IL, USA) package program. Homogeneity of variance was analyzed by Levenne test and normal distribution was analyzed by Shapiro-Wilk test. One Way ANOVA Test was used to analyze all parameters. Significance was determined at p<0.05. No statistically significant difference was detected between ACTN3 rs1815739, PPARA rs4253778 and IL6 rs1800795 polymorphisms and aerobic performance parameters (p>0.05). In the findings, no statistically significant difference was detected between the relevant polymorphisms and aerobic performance parameters in our cohort. In conclusion, ACTN3 rs1815739, PPARA rs4253778 and IL6 rs1800795 genes did not create any difference in aerobic endurance in volleyball players. Our results need to be confirmed by further studies.

Anahtar Kelimeler

Athlete, power, polymorphism, volleyball

Proje Numarası

GÜBAP2902-21.A0310.02.01

Kaynakça

• Rivera MA., Dionne FT., Wolfarth B., Chagnon M., Simoneau JA., Perusse L., Bouchard C. (1997). Muscle-specific creatine kinase gene polymorphisms in elite endurance athletes and sedentary controls. Medicine and Science in Sports and Exercise. 29(11), 1444-1447.
• Lucia A., Gómez-Gallego F., Barroso I., Rabadán M., Bandrés F., San Juan AF., Franks PW. (2005). PPARGC1A genotype (Gly482Ser) predicts exceptional endurance capacity in European men. Journal Of Applied Physiology. 99(1), 344-348.
• Bray MS., Hagberg JM., Perusse L., Rankinen T., Roth SM., Wolfarth B., Bouchard C. (2009). The human gene map for performance and health-related fitness phenotypes: the 2006-2007 update. Medicine & Science in Sports & Exercise. 41(1), 34-72.
• Ulucan K. (2016). Spor genetiği açısından Türk sporcuların ACTN3 R577X polimorfizm literatür özeti. Clinical and Experimental Health Sciences. 6(1), 44-47.
• Ulucan K., Bayyurt GM., Konuk M., Güney AI. (2014). Effect of alpha-actinin-3 gene on Turkish trained and untrained middle school children’s sprinting performance: a pilot study. Biological Rhythm Research. 45(4), 509- 514.
• Ulucan K., Topal ES., Aksulu BK., Yaman B., Ciftci İC., Bıyıklı T. (2015). Atletik performans, genetik ve gen dopingi. İstanbul Kanuni Sultan Süleyman Tıp Dergisi. 7(2), 58-62.
• Arica S., Solgun HA., Demirel AD., Bakmaz E., Akçay T., Kapici S., Ulucan K. (2018). -174 G/C polymorphism of interleukin 6 gene is not significantly different in Turkish professional short and long distance runners. Cellular and Molecular Biology (Noisy-le-Grand, France). 64(11), 85-87.
• Eroğlu O., Zileli R., Nalbant MA., Ulucan K. (2018). Prevalence of alpha actinin-3 gene (ACTN3) R577X and angiotensin converting enzyme (ACE) insertion/deletion gene polymorphisms in national and amateur Turkish athletes. Cellular and Molecular Biology (Noisy-le-Grand, France). 64(5), 24-28.
• Dinç N., Gökmen MH. (2019). Atletik performans ve spor genetiği. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 6(2), 127-137.
• Akkoç O., Birlik A., Doğan CS., Kırandı Ö., Ulucan K. (2020). Türk ironman triatlon sporcularında IL-6, HIF1A, MCT1, PPAR-a Polimorfizm dağılımının belirlenmesi. Spor Eğitim Dergisi. 4(1), 1-7.
• Aslan BT., Eken BF., Ulucan K. (2019). Genetic analysis of differences of exercise training adaptations. Niğde Üniversitesi Beden Eğitimi ve Spor Bilimleri Dergisi. 13(2), 66-79.
• Pimenta EM., Coelho DB., Veneroso CE., Coelho EJB., Cruz IR., Morandi RF., Fernández JADP. (2013). Effect of ACTN3 gene on strength and endurance in soccer players. The Journal of Strength & Conditioning Research. 27(12), 3286-3292.
• Ulucan K., Yuksel I., Dogan CS., Kavas NC., Bilici MF., Kaynar O. (2020). Interleukin-6 rs1800795 polymorphism is not considered as a genetic biomarker in Turkish national skiing running athlete cohort. Fresenius Environmental Bulletin. 29, 6359-6362.
• Kazanci D., Polat T., Doğan CS., Aslan BT., Oktay S., Bilici MF., Kaynar Ö., Eken BF., Ulucan K. (2021). The determination of Il-6 rs1800795 polymorphism distribution in turkish national cross-country skiing athletes sub-groups created referring to the 1km CCSTAs. Clinical and Experimental Health Sciences. 11(4), 782-786.
• Braissant O., Foufelle F., Scotto C., Dauca M., Wahli W. (1996). Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-alpha, -beta, and -gamma in the adult rat. Endocrinology, 137(1). 354-366.
• Russell AP., Feilchenfeldt J., Schreiber S., Praz M., Crettenand A., Gobelet C., Meier CA., Bell DR., Kralli A., Giacobino JP., Dériaz O. (2003). Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-γ coactivator-1 and peroxisome proliferator-activated receptor-α in skeletal muscle. Diabetes. 52(12), 2874-2881.
• Schmitt B., Flück M., Décombaz J., Kreis R., Boesch C., Wittwer M., Hoppeler H. (2003). Transcriptional adaptations of lipid metabolism in tibialis anterior muscle of endurance-trained athletes. Physiological Genomics. 15(2), 148-157.
• Akçamlı D., Sipahi S., Yüksel İ., Kavas NC., Polat T., Sercan C., Ulucan K. (2018). Futbolcularda peroksizom proliferatör–aktive reseptör alfa rs4253778 polimorfizm dağılımının belirlenmesi. Eurasian Research in Sport Science. 3(2), 75-79.
• Kirişci İ., Bilal G. (2022). Sezon öncesi hazırlık çalışmalarının genç voleybolcuların seçilmiş performans parametrelerine etkisi. Herkes için Spor ve Rekreasyon Dergisi. 4(2), 47-51.
• Karacabey K., Paşaoğlu A. (2011). Voleybol antrenman teorisi ve antrenör. Bedray Basın Yayıncılık. İstanbul.
• Ruiz JR., Fernández del Valle M., Verde Z., Díez‐Vega I., Santiago C., Yvert T., Lucia A. (2011). ACTN3 R577X polymorphism does not influence explosive leg muscle power in elite volleyball players. Scandinavian Journal of Medicine & Science in Sports. 21(6), 34-41.
• Elgin E., Dinc N., Yücel SB., Taneli F., Durmaz B. (2020). ACTN3 R577X polymorphism does not ınfluence explosive leg muscle power in elite turkish volleyball players. International Journal of Applied Exercise Physiology. 9(10), 245-252.
• Krustrup P., Mohr M., Amstrup T., Rysgaard T., Johansen J., Steensberg A., Bangsbo J. (2003). The yo-yo intermittent recovery test: physiological response, reliability, and validity. Medicine & Science in Sports & Exercise. 35(4), 697-705.
• Eken BF., Yılmaz ÖÖ., Polat T., Aslan BT., Ulucan K. (2021). Türk Futbolcularda Alfa-Aktinin-3 (ACTN3) ve anjiyotensin dönüştürücü enzim (ACE) polimorfizmleri atletik performans için bir biyobelirteç olabilir mi?. Eurasian Research in Sport Science. 6(2), 147-159.
• Berman Y., North KN. (2010). A gene for speed: The emerging role of α-actinin-3 in muscle metabolism. The Journal of Physiology. 25(4), 250-259.
• Santiago C., Rodríguez‐Romo G., Gómez‐Gallego F., González‐Freire M., Yvert T., Verde Z., Lucia A. (2010). Is there an association between ACTN3 R577X polymorphism and muscle power phenotypes in young, non‐athletic adults?. Scandinavian Journal of Medicine & Science in Sports. 20(5), 771-778.
• Saunders CJ., September AV., Xenophontos SL., Cariolou MA., Anastassiades LC., Noakes TD., Collins M. (2007). No association of the ACTN3 gene R577X polymorphism with endurance performance in Ironman Triathlons. Annals of Human Genetics. 71(6), 777-781.
• Chan S., Seto JT., Houweling PJ., Yang N., North KN., Head SI. (2011). Properties of extensor digitorum longus muscle and skinned fibers from adult and aged male and female Actn3 knockout mice. Muscle & Nerve. 43(1), 37-48.
• Quinlan KG., Seto JT., Turner N., Vandebrouck A., Floetenmeyer M., Macarthur DG., North KN. (2010). α-Actinin-3 deficiency results in reduced glycogen phosphorylase activity and altered calcium handling in skeletal muscle. Human Molecular Genetics. 19(7), 1335-1346.
• Lopez-Leon S., Tuvblad C., Forero DA. (2016). Sports genetics: the PPARA gene and athletes’ high ability in endurance sports. A systematic review and meta-analysis. Biology of Sport. 33(1), 3-6.
• Ahmetov II., Mozhayskaya IA., Flavell DM., Astratenkova IV., Komkova AI., Lyubaeva EV., Rogozkin VA. (2006). PPARα gene variation and physical performance in Russian athletes. European Journal of Applied Physiology. 97, 103-108.
• Petr M., Maciejewska-Skrendo A., Zajac A., Chycki J., Stastny P. (2019). Association of elite sports status with gene variants of peroxisome proliferator activated receptors and their transcriptional coactivator. International Journal of Molecular Sciences. 21(1), 162.
• Ahmetov II., Fedotovskaya ON. (2012). Sports genomics: Current state of knowledge and future directions. Cellular and Molecular Exercise Physiology. 1(1), e1.
• Keller P., Penkowa M., Keller C., Steensberg A., Fischer CP., Giralt M., Hidalgo J., Pedersen BK. (2005). Interleukin‐6 receptor expression in contracting human skeletal muscle: regulating role of IL‐6. Federation of American Societies for Experimental Biology (FASEB) Journal. 19(9), 1181-1183.
• Tuna G., Polat T., Yilmaz ÖÖ., Kapici S., Doğan CS., Sağiroğlu I., Ulucan K. (2022). The relationship between swimming styles and Il-6 Rs1800795 polymorphism in professional swimmers. Pakistan Journal of Medical & Health Sciences. 16(07), 444-446.
• Eliakim A., Portal S., Zadik Z., Meckel Y., Nemet D. (2013). Training reduces catabolic and inflammatory response to a single practice in female volleyball players. The Journal of Strength & Conditioning Research. 27(11), 3110-3115.