Exploring the Complementary Influence of an Oleuropein-Enriched Phytochemical Extract on Neuro-biochemistry, Behavior, and Histopathology in Aging and Depressed Male Rats

Yıl 2025, Cilt: 8 Sayı: 2, 58 - 69, 15.03.2025

Shirin Tarbiat , Kübra Ergün , Refika Aydoğdu , Estelle Larissa Ndengom Djomaleu , Kürşat Filikci

https://doi.org/10.19127/bshealthscience.1579376

Öz

Aging correlates with neurodegenerative diseases such as depression, marked by neuro-biochemical changes triggering neuronal loss. Nutraceuticals exhibit antioxidant and neuroprotective properties and offer potential intervention. Our study examines the preventive effects of an olive leaf extract consisting of 20% pure oleuropein in an animal model of aging and depression. Aging in Wistar rats was induced by intraperitoneal injections of 150 mg of D-galactose/kg body weight for 6 weeks. The depression was caused by forced swimming. We examined Monoamine oxidase and acetylcholinesterase activities, histopathology of the brain, and the serum plasminogen activator inhibitor-1, superoxide dismutase, and monoaldehyde levels of all animal groups. Depressed and aged rats showed increased Monoamine oxidase, acetylcholinesterase, and plasminogen activator inhibitor-1 levels, and a decrease in superoxide dismutase levels. The administration of 200 mg of extract before depression or aging inductions significantly reduced the Monoamine oxidase and acetylcholinesterase activities. Degeneration/necrosis was observed in neurons of all experimental animal groups. We demonstrate that the olive leaf extract with 20% pure oleuropein markedly maintained the neurotransmitter levels in the brain and enhanced the antioxidant defense system in depressed and aged animal models but was unable to yield behavioral benefits.

Anahtar Kelimeler

Oleuropein, Depression, Aging, Monoaminoxidase, Acetylcholinesterase

Etik Beyan

Üsküdar Üniversitesi Hayvan Laboratuvarı Etik Kurulu (UU-HADYEK 2020-06), tüm deneysel prosedürleri onayladı.

Destekleyen Kurum

Üsküdar Üniversitesi

Proje Numarası

No financial grant was received for this research.

Teşekkür

Ayrıca bu çalışmayı gerçekleştirmemize olanak sağlayan gerekli imkân ve çalışma ortamını sağlayan Üsküdar Üniversitesi ve Ankara Üniversitesi'ne de teşekkür etmek isteriz.

Kaynakça

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