Yeni bir kombinasyon folklorik şifalı bitki ekstraktının sıçanlarda ortopedik olarak genişletilmiş sütürde kemik oluşumu üzerindeki etkileri

Yıl 2023, Cilt: 7 Sayı: 2, 106 - 113, 30.08.2023

Şeref Ezirganlı , Hakan Özdemir , Muhammed Birlik Oguz Kazancioglu , Sertaç Aksakallı , Mukaddes Eşrefoğlu

https://doi.org/10.34084/bshr.1284711

Öz

Amaç: Bu çalışmanın amacı, sıçanlarda premaksiller sütür ekspansiyonunda yeni bir kombinasyon folkrorik tıbbi bitki ekstratının kemik iyileşmesi üzerindeki histolojik etkilerinin değerlendirmektir.
Materyal ve metod: Bu çalışmada otuz erkek Spraque-Dawley sıçanı kullanılmıştır. Çalışma her biri eşit olmak üzere 10 sıçandan oluşan bir kontrol iki deney grubuna ayrılmıştır. Sıçanlar sarmal yaylar ile premaksiller sütür genişlemesine tabi tutulmuştur. Tek genişleme grubu kontrol grubu olarak (Group A) tanımlanmıştır. Deney grubu OstokinPlus-10 (Group B) ve OstokinPlus-20 (Group C) olarak tanımlanmıştır. Deney gruplarında, 10 ve 20 ml/kg OstokinPlus bitkileri, çalışma süresi boyunca orogastrik tüp kullanılarak genişletildikten sonra sistemik olarak uygulanmıştır. Yaylar yerleştirilmiş ve 30 SN kuvveti sağlayacak şekilde etkinleştirilmiştir. 5 gün sonra, yaylar çıkarılmış ve kısa uzunluklarda dikdörtgen tespit teli ile değiştirilmiştir. Diş ayrımı 15 gün sürdürülmüştür. 15 günlük bir konsolidasyon periyodundan sonra, hayvanlara ötenazi uygulanmış ve midpalatal sütur kıkırdağını içeren maksiller kemik cerrahi olarak çıkarılmıştır. Örnekler, rejenere kemiğin histomorfometrik değerlendirmesi için hazırlanmıştır.
Bulgular: Aktive heliks yayı uygulamasının ardından midpalatal sütur başarılı bir şekilde ayrılmıştır. Ayrılan premaksiller sütur yeni kemik ve organize olmayan fibröz dokular ile dolmuştur. Yeni oluşan kemik yüzdesi ve kemik alanı arasında önemli farklılıklar bulunmuştur (p< 0.05). Araştırılan parametreler için Group B ve Group C, Group A daha iyi sonuçlar ortaya koymuştur.
Sonuç: OstokinPlus ekstaratı premaksiller sütur genişlemesi esnasında kemik iyileşmesi ve formasyonunu olumlu yönde etkilemiştir.

Anahtar Kelimeler

Hızlı maksiller genişleme, OstokinPlus bitkisi, kemik rejenerasyonu, histomorfometri

Destekleyen Kurum

herhangi bir kurum desteği bulunmamaktadır

Effects of a new combination folkloric medicinal plant extract on bone formation in orthopedically expanded suture in rats

Öz

Objectives. The aim of this study was to evaluate histological effects of a new combination folkloric medicinal plant extract on bone healing in premaxillary suture expansion in rats.
Materials and methods. Thirty male Sprague–Dawley rats were used in this study. Rats were evenly divided into three groups (one control and two experimental groups) of ten each. The animals were subjected to premaxillary suture expansion by helix springs. The only expansion group is defined as the control group (Group A). The experimental groups are defined as OstokinPlus-10 (Group B) and OstokinPlus-20 (Group C). In the experimental groups, 10 and 20 ml/kg OstokinPlus herbals were applied systemically after the expansion by use of an orogastric tube during the time of study. The springs were placed and activated to deliver a 30 cN force. After 5 days, the springs were removed and replaced with short lengths of rectangular retaining wire. Tooth separation was maintained for 15 days. After a consolidation period of 15 days, the animals were euthanatized and the maxillary bone containing the midpalatal suture cartilage was surgically removed. The specimens were prepared for histomorphometric assessment of the regenerated bone.
Results. The midpalatal suture was successfully distracted following application of the activated helix spring. The distracted premaxillary suture was filled with new bone formation and unorganized fibrous tissues. Newly formed bone percentage and the bone area were found to have significant differences (p< 0.05). For investigated parameters, Group B and Group C revealed more positive results than Group A.
Conclusion: OstokinPlus herbal had positive effects on bone healing and formation during premaxillary suture expansion

Anahtar Kelimeler

Rapid maxillary expansion, OstokinPlus herbal, Bone regeneration, Histomorphometry

Kaynakça

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