Hatay’da Yetişen Verbascum tripolitanum’un Bazı Kimyasal ve Biyolojik Özellikleri

Yıl 2019, Cilt: 3 Sayı: 2, 79 - 82, 20.12.2019

Hatice Danahaliloğlu , Yener Tekeli Yelda Güzel

https://doi.org/10.31594/commagene.598450

Öz

Bu çalışmada,
Hatay bölgesinde yetişen Verbascum tripolitanum’un antioksidan ve antimikrobiyal
özellikleri, yağ asitleri içeriği belirlenmiştir. Toplam fenolik madde miktarı Folin-Ciocaltaeu
yöntemi ile tayin edilmiştir. Antioksidan aktivite DPPH serbest radikal süpürme
yöntemi, Fe⁺³ indirgeme metodu (FRAP), bakır iyonu indirgeme metodu (CUPRAC),
ve β-karoten-linoleik asit emülsiyon yöntemleri ile test edilmiştir. V. tripolitanum’un antimikrobiyal etkisi 2 gram pozitif
bakteri (Staphylococcus aureus, metisiline dirençli S. aureus), 2 gram negatif bakteri (Pseudomonas aeruginosa, Escherichia coli) ve 1 maya türüne (Candida
albicans) karşı mikrodilüsyon broth metodu ile analiz edilmiştir. Yağ asitleri
analizi için GC-FID kullanılmıştır.

Anahtar Kelimeler

Verbascum tripolitanum, antioksidan, antimikrobiyal, yağ asitleri

Destekleyen Kurum

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

243

Teşekkür

Bu çalışmaya destek olan Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimine teşekkür ederiz (Proje no: 243).

Some Chemical and Biological Properties of Verbascum tripolitanum Growing in Hatay

Öz

In this study, antioxidant
and antimicrobial properties and fatty acid compositions of Verbascum tripolitanum
were determined. Total
phenolic contents were determined by Folin-Ciocaltaeu method. Antioxidant activity
was tested with DPPH free radical scavenging assay, ferric ion reducing power (FRAP),
cupric reducing antioxidant capacity (CUPRAC), and β-carotene-linoleic acid emulsion
method. The antimicrobial effects of the V. tripolitanum were tested
by the microdilution broth method against two gram-positive bacterias (Staphylococcus
aureus, Methicillin-resistant S. aureus), two gram-negative bacterias
(Pseudomonas aeruginosa, Escherichia coli), and one kind of yeast
(Candida albicans). The fatty acid compositions were determined by GC-FID.

Anahtar Kelimeler

Verbascum tripolitanum, antioxidant, antimicrobial, fatty acid

Proje Numarası

243

Kaynakça

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