Malat dehidrogenaz/malik enzim: insan folikül sıvısında NADPH üreten diğer bir enzim mi?

Yıl 2017, Cilt: 42 Sayı: 3, 413 - 418, 30.09.2017

Hülya Leventerler , İbrahim Ferhat Ürünsak Ümit Yaşar Mehmet Turan Çetin Nurten Dikmen

https://doi.org/10.17826/cutf.323368

Öz

Amaç: Bu çalışmanın amacı, üremeye yardımcı tedavi merkezine başvuran hastaların folikül sıvı örneklerinde malat dehidrogenaz ve malik enzim aktivitelerini inceleyerek oosit ve embriyo gelişimi üzerindeki olası etkilerini araştırmaktır.

Gereç ve Yöntem: Otuz hasta yaşlarına göre 3 gruba ayrıldı. Hastaların folikül sıvı örneklerinde malat dehidrogenaz ve malik enzim aktivite değişimleri incelenerek serum folikül stimülan hormon ve estradiol düzeyleri ve diğer kriterlerle (yaş,  toplanan oosit sayısı, gelişen embriyo sayısı, 1. kalite embriyo sayısı,  2. kalite embriyo sayısı) ilişkisi ve embriyo gelişimi üzerindeki olası etkileri karşılaştırıldı.

Bulgular: Malat dehidrogenaz-NADP aktivitesi ve estradiol düzeyi arasında zayıf fakat anlamlı bir korelasyon saptandı. En yüksek malat dehidrogenaz-NAD aktivitesi ve estradiol düzeyi ikinci grupta (30-45 yaş aralığı) bulundu. Bu grupta malat dehidrogenaz-NAD aktivitesi ve estradiol düzeyi arasında önemli bir korelasyon saptanmadı.

Sonuç: NADPH, memeli oosit ve embriyolarında, lipit  ve steroidal hormon sentezi ile enzimatik antioksidan koruma sistemleri için önemli bir kofaktördür. Sonuçlarımıza göre, insan foliküler sıvısında malat dehidrogenaz aktivitesinin varlığının gösterilmesi glukoz-6-fosfat dehidrogenaz aktivitesine ek olarak alternatif bir NADPH kaynağı olabileceğini düşündürmüştür.

Anahtar Kelimeler

Enzim, folliküler sıvı, oksidatif stres, oosit

Malate dehydrogenase/malic enzyme: is it another NADPH generating enzyme in human follicular fluid?

Öz

Purpose: The purpose of this study was to determine malate dehydrogenase and malic enzyme activities in human follicular fluid of patients who attended the assisted reproductive unit and to investigate their relation with oocyte and embryo development.

Materials and Methods: Thirty patients were classified into three groups with respect to their age. Changes in follicular fluid malate dehydrogenase and malic enzyme activity were analyzed and compared with serum follicle stimulating hormone and estradiol levels and other criteria (age, number of retrieved oocytes, number of developed embryos, number of grade 1 embryo and grade 2 embryo) to determine the possible effects on embryo development.

Results: A weak but significant correlation was detected between malate dehydrogenase-NADP activity and estradiol level.  malate dehydrogenase-NAD activity and estradiol level were highest in the second group (age between 30-45). But no significant correlation was determined between malate dehydrogenase-NAD activity and estradiol level in this group.

Conclusion: NADPH is an important cofactor for lipid and steroidal hormon synthesis and enzymatic antioxidant defence systems in mammalian oocytes and embryos. According to our results, we believe that the presence of malate dehydrogenase activity in human follicular fluid may be an alternative supplier of NADPH in addition to glucose-6-phosphate dehydrogenase activity.  

Anahtar Kelimeler

Enzyme, follicular fluid, oxidative stres, oocytes

Kaynakça

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