Kemik iliği kaynaklı mezenkimal kök hücrelerin deneysel Asherman modeli üzerine antiapoptotik ve proliferatif etkisi

Yıl 2019, Cilt: 44 Sayı: Ek 1, 434 - 446, 29.12.2019

Şamil Öztürk , Pınar Kılıçaslan Sönmez , İlhan Özdemir Yunus Emre Topdağı , Mehmet İbrahim Tuğlu

https://doi.org/10.17826/cumj.573200

Cited By: 4

Öz

Amaç: Bu çalışmada Asherman sendromundaki (AS)endometriyal hasarlarda cerrahi yöntemlere ve medikal tedavilere alternatif olarak kök hücre tedavisinin etkileri araştırılmıştır.  

Gereç ve Yöntem: Bu çalışmada sıçanlarda AS modeli kimyasal olarak oluşturuldu. Aynı türün erkek bireylerinin tibia ve femur kemiğinden izole edilen kemik iliği kaynaklı mezenkimal kök hücreler (KİMKH) AS oluşturulan dişi sıçanlara verilerek endometriyumda meydana gelen değişiklikler histopatolojik parametrelerle değerlendirildi. Asherman+ besiyeri, Asherman+ niş, Asherman+ KİMKH, Asherman+ KİMKH+ niş olmak üzere toplam dört grup oluşturuldu.

Bulgular: Tedavi gruplarında epitel ve lamina propriyadaki rejenerasyonla birilkte endometriyal kalınlığın, bez sayısının ve vaskülarizasyonun arttığı, fibröz alanların ve apoptotik hücre ölümünün azaldığı  gözlenmiştir. Kontrol grubu olarak değerlendirilen sağ uterin hornlarda ise her hangi bir histopatolojik değişiklik görülmemiştir.

Sonuç: KİMKH ve Niş uygulamalarının, infertiliteye neden olan mekanizmaların içerisinde yer alan adezyon oluşumunu azaltarakedavi ile kliniğe katkı sağlayabileceği gösterildi. Bu olumlu sonuçlar Asherman çalışmalarının kliniğe taşınabilmesi açısından ümit vericidir.

Anahtar Kelimeler

Mezenkimal kök hücre, Asherman sendromu, Apoptozis, İnfertilite, , Histoloji

Destekleyen Kurum

Kurum veya şahıs desteği alınmamıştır.

Antiapoptotic and proliferative effect of bone marrow-derived mesenchymal stem cells on experimental Asherman model

Öz

Purpose: We investigated the effects of stem cell therapy as an alternative to surgical methods and medical treatments in endometrial injuries in Asherman syndrome (AS).

Materials and Methods: In this study, AS model was created chemically in rats. The bone marrow-derived mesenchymal stem cells isolated from the tibia and femoral bone of male individuals of the same species (BMDSC) were given to female rats with asherman syndrome and the changes in the endometrium were evaluated by histopathological parameters. Asherman + medium, Asherman + niche, Asherman + BMDSCs, Asherman + BMDSCs + niche were formed in four groups.

Results: It was observed that increased endometrial thickness, gland count and vascularization and decreased fibrous areas and apoptotic cell death with regeneration in epithelium and lamina propria in treatment groups. No histopathologic changes were observed in the right uterine horns, which were evaluated as control group. . 

Conclusion: BMDSCs and Niche applications can contribute to the clinic by reducing the formation of adhesion within the mechanisms causing infertility. These positive results are promising in terms of transporting Asherman studies to the clinic.It has been shown that BMDSCs and Niche may contribute to the clinic by treatment with adhesion molecules in mechanisms that cause infertility.

Anahtar Kelimeler

Mesenchymal stem cell, Asherman syndrome, Apoptosis, Infertility, Histology

Kaynakça

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