Antioxidant effects of resveratrol on vas deferens contractility in streptozotocin-induced diabetic rats

Year 2020, Volume: 45 Issue: 1, 316 - 323, 31.03.2020

Tuğçe Demirtaş Şahin

https://doi.org/10.17826/cumj.656102

Abstract

Purpose: The aim of the current study is to investigate the antioxidant effects of resveratrol on vas deferens contractility in streptozotocin-induced diabetic rats.
Materials and Methods: Adult male Wistar Albino rats were separated into three groups: control, diabetic and resveratrol-treated diabetic. Diabetes was induced by a single i.p. injection of STZ (50 mg/kg), and 2 days after the STZ injection the rats having hyperglycemia (>300 mg/dl) were assigned to be diabetic. Rats in treatment group were administered resveratrol (20 mg/kg/day, i.p.) for 4 weeks. After 4 weeks, vas deferens tissues were isolated and, noradrenaline-, ATP- and electrical field stimulation (EFS)-induced responses were obtained from these tissues.
Results: Noradrenaline- and ATP-induced responses significantly increased and EFS-induced responses significantly decreased in diabetic rats compared to control rats. EFS-induced responses in diabetic rats were inhibited more than control rats in the presence of pyrogallol. The increase in EFS-induced responses in the presence of tempol was found markedly enhanced in diabetic rats compared to control rats. Resveratrol treatment significantly ameliorated all the aforementioned responses.
Conclusion: This study demonstrated that resveratrol restored impaired contractility of vas deferens in diabetic rats by its antioxidant effects, suggesting that oxidative stress may play a crucial role on the development of ejaculatory dysfunctions associated with diabetes and antioxidant treatment may be effective in these dysfunctions.

Keywords

Diabetes mellitus, vas deferens, resveratrol

Resveratrolün streptozotosin ile diyabet oluşturulan sıçanlarda vas deferens kontraktilitesi üzerindeki antioksidan etkileri

Abstract

Amaç: Bu çalışmanın amacı, streptozotosin (STZ) ile diyabet oluşturulmuş sıçanlarda vas deferens kontraktilitesi üzerinde resveratrolün antioksidan etkilerini araştırmaktır.
Gereç ve Yöntem: Erişkin erkek Wistar Albino sıçanlar kontrol, diyabetik ve resveratrol tedavisi uygulanan diyabetik olarak üç gruba ayrılmıştır. Diyabet, tek doz i.p. STZ enjeksiyonuyla (50mg/kg) oluşturulmuş ve STZ enjeksiyonundan 2 gün sonra hiperglisemi (>300 mg/dl) olan hayvanlar diyabetik olarak kabul edilmiştir. Tedavi grubundaki sıçanlara 4 hafta boyunca (20 mg/kg/gün, i.p.) resveratrol uygulanmıştır. 4 hafta sonunda sıçanların vas deferens dokuları izole edilmiş ve bu dokularda noradrenalin, ATP, elektriksel alan uyarısı (EAU) aracılı yanıtlar elde edilmiştir.
Bulgular: Diyabetik sıçanlarda kontrol grubuna göre noradrenalin ve ATP aracılı yanıtlar anlamlı olarak artarken, EAU aracılı yanıtlar azalmıştır. EAU aracılı yanıtlar pirogallol varlığında diyabetik sıçanlarda kontrol sıçanlarına göre daha fazla inhibe olmuştur. Tempol varlığında ise EAU aracılı yanıtlardaki artış, diyabetik grupta kontrol grubuna göre anlamlı olarak fazla bulunmuştur. Resveratrol tedavisi, diyabetik sıçanlarda artan noradrenalin ve ATP aracılı yanıtları anlamlı olarak azaltmış, azalan EAU aracılı yanıtları ise artırmıştır.
Sonuç: Bu çalışma resveratrol tedavisinin diyabetik sıçanlarda vas deferens kontraktilitesindeki bozukluğu antioksidan etkileri aracılığıyla düzelttiğini ortaya koymuştur. Sonuçlar, diyabet ile ilişkili ejakülasyon bozukluklarında oksidatif stresin rol oynayabileceğini ve antioksidan tedavinin bu bozukluklarda etkili olabileceğini düşündürmektedir.

Keywords

Diabetes mellitus, vas deferens, resveratrol

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