Levels of oxidative stress and apoptosis-related biomarkers in endometriosis

Yıl 2023, Cilt: 48 Sayı: 2, 480 - 488, 02.07.2023

Dilara Ülger Özbek , Savaş Karakuş , Sevtap Bakır

https://doi.org/10.17826/cumj.1247227

Öz

Purpose: In this study, the apoptosis marker M30, the oxidative stress markers malondialdehyde, (MDA) and asymmetric dimethyl arginine (ADMA) have been studied in the context of endometriosis.
Materials and Methods: This prospective case-control study comprises 31 patients diagnosed with endometriosis and 31 controls. ADMA and M30 levels in blood serum were measured by the enzyme-linked immunosorbent assay (ELISA) method, and MDA levels were measured by the spectrophotometric method. In addition, some biochemical parameters and cancer antigen-125 (CA-125) levels were also measured.
Results: M30 levels were statistically lower in endometriosis patients (271.5 IU/L) than in controls (371.3 IU/L). ADMA levels were higher in endometriosis patients (19.3 ng/L) compared to controls (12.7 ng/L). CA-125 levels were statistically higher in the endometriosis patients (65.1 U/mL) compared to the controls (19.0 U/mL). There was no significant difference between the two groups in MDA levels. The results regarding dyspareunia, pelvic pain, AST, and ALP were statistically significant.
Conclusion: In our study, decreased M30 levels in the patient group were associated with reduced apoptosis in endometriosis. ADMA levels, elevated with the increase of oxidative stress, were higher in the patients. MDA levels, an indicator of increased oxidative stress, were also higher in the patient group. This study constitutes the first data regarding endometriosis patients' ADMA, M30, and MDA levels.

Anahtar Kelimeler

Apoptosis, ADMA, Endometriosis, MDA, M30

Destekleyen Kurum

Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

T-606

Teşekkür

The researchers thank Ziynet Çınar, Ph.D, for statistical analysis support. This study was supported as a Master Thesis by the Sivas Cumhuriyet University Scientific Research Projects (CÜBAP) project numbered T-606.

Endometrioziste oksidatif stres ve apoptozla ilişkili biyobelirteçlerin düzeyleri

Öz

Amaç: Bu çalışmada apoptoz belirteci M30 ve oksidatif stres belirteçleri malondialdehit (MDA) ve asimetrik dimetil arjinin (ADMA) endometriozis de düzeyleri incelenmiştir.
Gereç ve Yöntem: Bu prospektif vaka-kontrol çalışması, endometriozis tanısı alan 31 hasta ve 31sağlıklı kontrol grubundan oluşmaktadır. Kan serumundaki ADMA ve M30 düzeyleri enzim bağlı immünosorbent assay (ELISA) yöntemiyle, MDA düzeyleri ise spektrofotometrik yöntemle ölçüldü. Ayrıca bazı biyokimyasal parametreler ve kanser antijen (CA-125) düzeyleri de ölçüldü.
Bulgular: M30 düzeyleri endometriozis grubunda (271,5 IU/L), kontrol grubuna (371,3 IU/L) göre istatistiksel olarak daha düşük bulundu. ADMA seviyeleri endometriozis grubunda (19,3 ng/L) kontrol grubuna (12,7 ng/L) göre daha yüksekti. CA-125 düzeyleri endometriozis grubunda (65,1 U/mL) kontrol grubuna göre (19,0 U/mL) istatistiksel olarak yüksekti. MDA düzeylerinde iki grup arasında anlamlı fark yoktu. Sonuçlar disparoni, pelvik ağrı, AST ve ALP açısından istatistiksel olarak anlamlıydı.
Sonuç: Çalışmamızda hasta grubunda azalmış M30 seviyeleri endometrioziste azalmış apoptoz ile ilişkilendirildi. Oksidatif stresin artmasıyla yükselen ADMA düzeyleri hastalarda daha yüksekti. Artmış oksidatif stresin bir göstergesi olan MDA düzeyleri de hasta grubunda daha yüksek bulundu. Bu çalışma, endometriozis hastalarında ADMA, M30 ve MDA düzeylerinin bir arada yer alması açısından ilk verileri oluşturmaktadır.

Anahtar Kelimeler

Apoptoz, ADMA, endometriozis, MDA, M30

Proje Numarası

T-606

Kaynakça

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