Rosmarinik asidin sıçanlarda siklofosfamid ile indüklenen nefrotoksisite üzerine etkileri

Yıl 2023, Cilt: 48 Sayı: 3, 902 - 910, 30.09.2023

Dilan Çetinavcı , Engin Yenilmez , Ayşe Firuze Bıyık , Ahmet Alver , Neslihan Sağlam

https://doi.org/10.17826/cumj.1317508

Cited By: 1

Öz

Amaç: Siklofosfamid (CP) antineoplastik bir ajandır. Birçok kanser türünün tedavisinde kullanılmaktadır. Rosmarinik asit (RA), antiinflamatuar, antitümör, antibakteriyel ve antimikrobiyal etkiler gibi dikkate değer biyolojik aktiviteler sergiler. Bu çalışmanın amacı, rosmarinik asidin CP kaynaklı nefrotoksisiteye etkisini değerlendirmektir.
Gereç ve Yöntem: On sekiz erkek Sprague Dawley sıçanı rastgele 3 eşit gruba ayrıldı. Sham grubuna (n=6): %0,9 salin solüsyonu/8 gün boyunca/oral + %0,9 salin solüsyonu/8. gün/intraperitoneal; CP grubuna (n=6): %0,9 salin solüsyonu/8 gün boyunca/oral + 200 mg/kg/8. gün/intraperitoneal CP; CP+RA grubu (n=6): 100 mg/kg/8 gün boyunca/oral RA + 200 mg/kg/8. gün/intraperitoneal CP uygulandı. Toplanan dokularda Hematoksilen ve Eozin, Periyodik Asit-Schiff ve Masson Trikrom boyamaları yapıldı.
Bulgular: Histopatolojik değerlendirmede CP grubunda tübüler atrofi, glomerüler hasar, vasküler konjesyon, vakuolizasyon ve interstisyel inflamasyon saptandı. Histopatolojik skorlar CP+RA grubunda CP grubuna göre anlamlı derecede düşüktü. Sham grubu ile karşılaştırıldığında CP grubunda intertübüler fibrozis gözlendi. Rosmarinik asit ile fibrozis azaldı. PAS ile CP grubu kesitlerinde, tübüler epitel vakuolizasyonu, fırçamsı kenar ve bazal membran bozulması gözlendi. Bu sonuçlar rosmarinik asit ile azaldı. CP grubunda artan kan üre nitrojen değeri (BUN) CP+RA grubunda daha düşükken, CP grubunda azalan SOD değeri CP+RA grubunda daha yüksekti.
Sonuç: RA' nın böbrekte tübüler atrofi, glomerüler hasar, vasküler konjesyon, vakuolizasyon ve interstisyel inflamasyona neden olan CP' nin etkilerine karşı koruyucu etkileri bulunmaktadır.

Anahtar Kelimeler

Nefrotoksisite; siklofosfamid; sıçan; rosmarinik asit.

Destekleyen Kurum

Karadeniz Teknik Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

TTU-2018-8011

Effects of rosmarinic acid on cyclophosphamide-induced nephrotoxicity in rats

Öz

Purpose: Cyclophosphamide (CP) is an antineoplastic agent. It is used in the treatment of many types of cancer. Rosmarinic acid (RA) exhibits remarkable biological activities such as anti-inflammatory, antitumor, antibacterial, and antimicrobial effects. This study aimed to evaluate the effect of rosmarinic acid against CP-induced nephrotoxicity.
Materials and Methods: Eighteen male Sprague Dawley rats were randomly divided into 3 equal groups; Sham group (n=6): 0.9% saline solution/8 days/oral gavage + 0.9% saline solution/8th day/intraperitoneal, CP group (n=6): 0.9% saline solution/8 days/oral gavage + 200 mg/kg/8th day/intraperitoneal CP, and CP+RA group (n=6): 100 mg/kg/8 days/oral gavage RA + 200 mg/kg/8th day/intraperitoneal CP was applied. Hematoxylin and Eosin, Periodic Acid-Schiff, and Masson’s Trichrome staining were performed on the collected tissues
Results: Histopathological evaluation revealed tubular atrophy, glomerular damage, vascular congestion, vacuolization, and interstitial inflammation in the CP group. Histopathological scores were significantly lower in the CP+RA group compared to the CP group. Intertubular fibrosis was observed in the CP group compared to the Sham group. Fibrosis decreased with rosmarinic acid. PAS-stained sections from the CP group showed tubular epithelial vacuolization, brush border, and basal membrane disruption. These findings decreased with rosmarinic acid. The increased blood urea nitrogen level in the CP group was lower in the CP+RA group, while the decreased SOD level in the CP group was higher in the CP+RA group.
Conclusion: RA has protective effects against CP causing tubular atrophy, glomerular damage, vascular congestion, vacuolization, and interstitial inflammation in the kidney

Anahtar Kelimeler

Nephrotoxicity, Cyclophosphamide, Rat, Rosmarinic acid

Proje Numarası

TTU-2018-8011

Kaynakça

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