Karnosol ve karnosik asit küçük hücreli dışı akciğer kanseri tedavisinde umut verici bir antikanser ajanı olabilir

Yıl 2024, Cilt: 49 Sayı: 1, 81 - 88, 29.03.2024

Tuğçe Duran , Nadir Koçak , Mehmet Karaselek

https://doi.org/10.17826/cumj.1399224

Öz

Amaç: Bu çalışma, karnosol ve karnosik asidin küçük hücreli dışı akciğer kanseri (KHDAK) hücre dizileri üzerindeki olası antikanser ve apoptotik etkisini in vitro olarak değerlendirmeyi amaçladı.
Gereç ve Yöntem: Çalışmada üç farklı KHDAK hücre hattı (H441, H520 ve H661) kullanılmış olup, tüm hücre hatlarında uygulama (karnosol ve karnosik asit uygulanmış) ve kontrol (uygulama yapılmayan) grupları oluşturulmuştur. IC50 dozları KHDAK hücre hatlarında MTT yöntemiyle belirlendi ve apoptozla ilişkili genlerin (Bax, Bak, kaspaz-3, p53, c-Myc, Bcl-2 ve Bcl-XL) ekspresyon seviyelerindeki değişiklikler gerçek zamanlı polimeraz zincir reaksiyonu (qPCR) ile gösterildi ve apoptoz akış sitometrisi ile doğrulandı. Tedavi grubu ve kontrol grubu sonuçları karşılaştırıldı.
Bulgular: Karnosol ve karnosik asidin IC50 dozları sırasıyla belirlendi; H441 için 60 µM, H661 için 20 µM, H520 için 40 µM, H441 için 20 µM, H661 için 40 µM, H520 için 40 µM. Karnosol uygulaması, çalışılan akciğer kanseri hücre hatlarında Bax, Bak, kaspaz-3 ve p53 ekspresyonunu arttırdı ancak Bcl-XL ekspresyonunu azalttı. Karnosik asit tedavisi apoptotik Bax, Bak, kaspaz-3 ve p53 ekspresyonunu arttırdı ancak antiapoptotik Bcl-2 ve Bcl-XL ekspresyonunu azalttı (p<0.05).
Sonuç: Bu çalışmada karnosol ve karnosik asidin KHDAK hücreleri üzerinde apoptotik etkileri olduğu gösterilmiştir. Bu ajanlar haklarında daha detaylı çalışmalar yapılmasıyla gelecekte KHDAK tedavisinde kullanılabilecek umut verici bir ajanlar olabilecektir.

Anahtar Kelimeler

Apoptoz, karnosol, karnosik asit, biberiye, küçük olmayan akciğer kanseri

Carnosol and carnosic acid may be a promising anticancer agent in non-small cell lung cancer treatment

Öz

Purpose: This study aimed to evaluate the possible anticancer and apoptotic effect of carnosol and carnosic acid on non-small cell lung cancer (NSCLC) cell lines in vitro.
Materials and Methods: Three different NSCLC cell lines (H441, H520, and H661) were used in the study and treatment (carnosol and carnosic acid treatment) and control (no treatment) groups were established in all cell lines. The IC50 doses were determined by MTT method on NSCLC cell lines and the changes in expression levels of apoptosis-related genes (Bax, Bak, caspase-3, p53, c-Myc, Bcl-2, and Bcl-XL) were demonstrated by real-time polymerase chain reaction (qPCR), and apoptosis was confirmed by flow cytometry. The treatment group and control group results were compared.
Results: The IC50 doses of carnosol and carnosic acid were determined; 60 µM for the H441, 20 µM for the H661, 40 µM for the H520, 20 µM for the H441, 40 µM for the H661, 40 µM for the H520, respectively. Carnosol treatment increased Bax, Bak, caspase-3, and p53 expression but decreased Bcl-XL expression in studied lung cancer cell lines. Carnosic acid treatment increased apoptotic Bax, Bak, caspase-3, and p53 expression but decreased antiapoptotic Bcl-2 and Bcl-XL expression (p<0.05).
Conclusion: In this study showed that carnosol and carnosic acid have apoptotic effects on NSCLC cells. With more detailed studies on these agents, these come become a promising agent that can be used in NSCLC cancer treatment in the future.

Anahtar Kelimeler

Apoptosis, carnosol, carnosic acid, rosemary, non-small lung cancer

Kaynakça

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