Sıçanlarda doksorubisin kaynaklı testis hasarına karşı crocinin faydalı rolü: vimentin modülasyonuna ilişkin görüşler

Yıl 2024, Cilt: 49 Sayı: 2, 332 - 342, 30.06.2024

Melike Özgül Önal , Sara Asaad , Gürkan Yiğittürk , Volkan Yaşar , Yasemin Biçer , Dilan Çetinavcı , Eyüp Altınöz , Mehmet Demir , Hülya Elbe , Feral Öztürk

https://doi.org/10.17826/cumj.1420141

Öz

Amaç: Doksorubisin (DOX) kemoterapide kullanılan geniş spektrumlu bir antibiyotiktir. Yan etkileri tedaviyi kısıtlamaktadır. Crocin, hem antiinflamatuar hem de antioksidan aktiviteye sahip karotenoidlerden biridir. Sıçanlarda doksorubisin kaynaklı testis hasarına karşı Crosinin etkilerini değerlendirmeyi amaçladık.
Gereç ve Yöntem: Kırk adet Wistar sıçan dört gruba ayrıldı. Grup 1: Kontrol, Grup 2: Crosin, Grup 3: DOX, Grup 4: DOX+Crosin (tümü için, n=10). Testis dokuları Hematoksilen-Eozin ile boyandı. Seminifer tübüllerin çapları ölçüldü ve testisin ortalama histopatolojik hasar skoru (MHDS) ölçüldü. Sertoli hücrelerinde Vimentin ekspresyonu H-Score olarak hesaplandı. Testis dokularında Malondialdehit (MDA) ve Glutatyon (GSH), Katalaz (CAT) ve Süperoksit dismutaz (SOD) aktivitelerinin düzeyleri belirlendi. Toplam Antioksidan Durumu (TAS) ve Toplam Oksidan Durumu (TOS) da hesaplandı.
Bulgular: DOX grubunda atrofik seminifer tübüller görüldü. Hasarlanan tübüllerde ödem, vakuolizasyon ve dezorganizasyon mevcuttu. DOX grubu ve kontrol grupları için MHDS'ler sırasıyla 4,60±0,45 ve 0,20±0,13 idi. Bu grupların her ikisi de anlamlı bir fark gösterdi. Crocin kullanımı sonrası histopatolojik hasar skoru azaldı. DOX grubuyla karşılaştırıldığında DOX+Crosin grubunda Vimentin immün ekspresyon düzeyleri ve seminifer tübül genişliği anlamlı derecede artarken tübül hasarı önemli ölçüde azaldı. DOX tedavisi sonrasında MDA ve TOS düzeyleri anlamlı düzeyde artarken, GSH, SOD, CAT ve TAS düzeyleri anlamlı düzeyde azaldı. Crocin aldıktan sonra tüm biyokimyasal göstergeler büyük ölçüde iyileşti.
Sonuç: Crosin takviyesi, oksidan/antioksidan durumunu dengeleyerek DOX'un neden olduğu fonksiyonun testis hasarına karşı yararlı etkiler gösterdi.

Anahtar Kelimeler

Doksorubisin, Crosin, testis, vimentin, oksidatif stres.

Destekleyen Kurum

Karabuk University Scientifict Research Fund

Proje Numarası

TYL-2020-2298

Beneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulation

Öz

Purpose: Doxorubicin (DOX) is a wide-spectrum antibiotic used for chemotherapy. Its side effects limit treatment. Crocin is one of the carotenoids that has both anti-inflammatory and antioxidant activities. We aimed to evaluate the effects of crocin against doxorubicin-induced testicular damage in rats.
Materials and Methods: Forty Wistar rats were divided into four groups. Group 1: Control, Group 2: Crocin, Group 3: DOX, Group 4: DOX+Crocin (n=10, for all). Testis tissues were stained with Hematoxylin-Eosin. The diameters of seminiferous tubules were measured and the testicular mean histopathologic damage score (MHDS) was calculated. Vimentin expression in Sertoli cells was calculated as H-Score. Levels of Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT), and Superoxide dismutase (SOD) activities were determined in testis tissues. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) were also calculated.
Results: Atrophic seminiferous tubules were seen in the DOX group. Edema, vacuolization, and disorganization were present in the injured tubules. The MHDSs for the DOX group and control groups were 4.60±0.45 and 0.20±0.13, respectively. Both of these groups showed a significant difference. The histopathologic score was reduced after using crocin. Tubule damage considerably decreased while immunoexpression levels of vimentin and seminiferous tubule width significantly increased in the DOX+Crocin group compared to the DOX group. MDA and TOS levels were significantly increased after DOX treatment, and GSH, SOD, CAT, and TAS levels were significantly decreased. All biochemical indicators were greatly improved after receiving crocin.
Conclusion: Crocin supplementation exhibited adequate beneficial effects against the testicular damage of DOX-induced function by balancing the oxidant/antioxidant status.

Anahtar Kelimeler

Doxorubicin, crocin, testis, vimentin, oxidative stress.

Etik Beyan

The Zonguldak Bulent Ecevit University Experimental Animals Ethics Committee approved the study (Protocol No. 2021-09-01/04).

Destekleyen Kurum

The research was supported by Karabuk University Scientifict Research Fund.

Proje Numarası

TYL-2020-2298

Kaynakça

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