DIVERSITY AND RELATIONSHIPS OF PONCIRUS (Poncirus trifoliata L.) AND PONCIRUS HYBRIDS BY SSR MOLECULAR MARKERS

Yıl 2009, Cilt: 26 Sayı: 2, 30 - 41, 01.06.2009

İlknur Polat

Öz

In this study, genetic relationship and diversity were determined by SSR (simple sequence repeat) molecular markers technique among 59 Poncirus (Poncirus trifoliata L. Raf.) types/cultivars derived from selections, introduction and hybridization. In this work, 14 SSR primers (TAA1, CAC19, TAA45, CT21, CAC33, CAC39, TAA33, CCT01, CAG01, CAT01, ATC09, TAA52, TAA15 and CAGG9) of the used 26 SSR primers produced polymorphic fragments. Genetic relationship and distance were determined by using UPGMA (unweighted-pair group method with arithmetic average) and PCA (principal component analysis) analysis. The Dice’s similarity coefficient among Poncirus and their hybrids accessions ranged from 0.63 to 1.00 and matrix correlation was 0.84. While Poncirus Krides SRA 339 - M and Poncirus SEAB B 6C - BB1 (SRA) in a group; Poncirus Town (SRA) and Poncirus Jacobsen B 6C - Z19 (SRA) in a different group; Poncirus Dwarf (SRA) and Poncirus Yamaguchi B 6C - Z28 (SRA) in a different group with 100% genetic similarity were determined. On the contrary, Glenn citrangedin (CRC 3573) 63% genetic similarity is the farthest among 60 Poncirus.

Anahtar Kelimeler

Poncirus trifoliata L.; SSR; Genetic resource; Molecular marker

ÜÇ YAPRAKLI (Poncirus trifoliata L.) VE ÜÇ YAPRAKLI MELEZLERİ GRUBU TURUNÇGİLLERİN GENETİK AKRABALIK VE FARKLILIKLARININ SSR MOLEKÜLER MARKIRLARLA TANIMLANMASI

Öz

Çalışmada, seleksiyon, introduksiyon ve melezlemelerle elde edilmiş 59 adet üç yaprak tip/çeşidinin genetik farklılığı ve birbiriyle olan genetik yakınlığı SSR (simple sequence repeat) moleküler markır tekniği kullanılarak incelenmiştir. Kullanmış olduğumuz 26 adet SSR primerinden 14 tanesi (TAA1, CAC19, TAA45, CT21, CAC33, CAC39, TAA33, CCT01, CAG01, CAT01, ATC09, TAA52, TAA15 ve CAGG9) polimorfik bulunmuştur. UPGMA (unweighted-pair group method with aritmetic average) ve PCA (principal component analysis) analizleri sonucu bireylerin birbirleriyle olan genetik yakınlıkları ve uzaklıkları belirlenmiştir. Dice’ın benzerlik katsayısına göre benzerlik oranları 0.63-1.00 arasında değişim göstermiş, matriks korelasyon katsayısı 0.84 olarak bulunmuştur. Benzerlik oranı Poncirus Krides SRA 339 ile Poncirus SEAB B 6C - BB1 (SRA); Poncirus Town (SRA) ile Poncirus Jacobsen B 6C - Z19 (SRA); Poncirus Dwarf (SRA) ile Poncirus Yamaguchi B 6C - Z28 (SRA) arasında ve 1.00 (% 100) oranında olduğu tespit edilmiştir. Glenn citrangedin (CRC 3573) ise % 63 oranıyla 60 birey içerisinden en uzak bireyi oluşturmaktadır.

Anahtar Kelimeler

Poncirus trifoliata L., SSR, Genetik kaynaklar, Moleküler markır

Kaynakça

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