Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi

Yıl 2019, Cilt: 36 Sayı: 2, 141 - 145, 10.12.2019

Sevinç Kıran , Ş.şebnem Ellialtıoğlu

https://doi.org/10.16882/derim.2019.611536

Öz

Bu çalışmanın amacı; Verticillium solgunluğuna dayanımın, bitki dokularında sentezlenen
fitoaleksin miktarıyla ilişkisini araştırmaktır. Çalışmada Long Purple patlıcan
çeşidi (hastalığa duyarlı) ile S. sisymbriifolium
yabani türü (hastalığa dayanıklı) bitkisel materyal olarak kullanılmıştır.
Elisitör olarak, araşidonik asit (5 ve 10 mM) çalışmada yer almıştır.
Elisitör ile uyarım işlemi 24, 48 ve 72 saat olarak uygulanmıştır. Ekstrakte
edilen maddeler gaz likit kromotografisi (GLC) cihazında analiz edilmiştir.
Dayanıklı türün kallus süspansiyon kültüründe fitoaleksin oluşumu yeterli
düzeyde uyarılamamıştır. Elde edilen bulgular araşidonik asidin kallus
süspansiyon kültüründe sadece Long Purple çeşitinde fitoaleksinlerin oluşumunu
uyarmada etkili olduğu, in vitro
koşularda doku fazında solavetivon ve süzüntü fazında ise lubimin maddelerinin
biriktiği belirlenmiştir. En yüksek madde birikimleri her iki fazda ‘10 mM
araşidonik asit x 72 saat uyarı süresi’ kombinasyonunda elde edilmiş, ‘doz x
uygulama süresi’ etkileşimi önemli bulunmuştur.

Anahtar Kelimeler

Dayanıklılık, Kallus kültürü, Lubimin, Patlıcan, Solavetivon

Destekleyen Kurum

Ankara Üniv. Fen Bilimleri Enst. (doktora tezi)

The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture

Öz

The
aim of this study was to investigate the relationship between the resistance of
Verticillium wilt and the amount of
phytoalexin, which is the defense molecules synthesized in plant tissues. In
this study, Long Purple eggplant variety (disease susceptible) and S. sisymbriifolium wild type (disease
resistant) were used as plant material. As elicitor, arachidonic acid (5 and
10 mM) was included in the study. Stimulation with elicitor lasted 24, 48
and 72 hours. Extracted substances were analyzed in gas liquid chromatography
(GLC). The formation of phytoalexin was not adequately stimulated in the callus
suspension culture of the resistant species. The results showed that
arachidonic acid was effective in stimulating the formation of phytoalexins in
the callus suspension culture in Long Purple variety, and in the in vitro conditions solavetivone was
formed in the tissue phase and lubimin substances in the filtrate
phase. The highest material accumulations were obtained in both phases:
’10 mM arachidonic acid x 72 hours’ ‘elicitor dose x stimulation period’
interaction was found to be significant.

Anahtar Kelimeler

Callus culture, Eggplant, Lubimin, Resistance, Solavetivone

Kaynakça

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