Orta Düzey 17α-Etinilestradiol ve 4-n-Nonilfenol Konsantrasyonlarının Chalcalburnus tarichi (Pallas, 1811) (Cyprinidae)’nin Primer Hepatositlerinde Apoptoz Üzerine Olan Etkileri

Yıl 2021, Cilt: 4 Sayı: 1, 8 - 18, 18.12.2021

Burak Kaptaner , Güler Ünal

Öz

Bu çalışmada 17α-etinilestradiol (EE2) ve 4-n-nonilfenol (NP)’ün düşük konsantrasyonlarının, Chalcalburnus tarichi (Pallas, 1811) (Cyprinidae)’den izole edilen hepatositlerde, apoptozis üzerine olan etkileri araştırıldı. Bu amaç doğrultusunda, EE2 ve NP’nin 0.1, 1 ve 10 µM konsantrasyonları hücrelere, 24 saat süre ile uygulandı. Daha sonra hücreler TUNEL ve propidyum iyodür ile boyandı ve apoptotik hücreler akım sitometrisi ile analiz edildi. Elde edilen sonuçlara göre apoptotik hücre yüzdesi her iki bileşiğin bütün konsantrasyonlarında arttı ancak bu artışların istatistiksel olarak anlamlı olmadığı belirlendi. Öte yandan EE2 ve NP’nin 10 µM konsantrasyonunda, apoptotik hücre yüzdesinde, kontrol grubuna kıyasla, istatistiksel olarak anlamlı olma yönünde eğilim gösteren yükselişler gözlendi (sırasıyla; P = 0.08 ve P = 0.12). Elde edilen bulgular hem EE2’nin hem de NP’nin balığın hepatositlerinde, karaciğer toksisitesi ile ilişki olarak, apoptozu uyarabilme potansiyeline sahip olduklarını göstermektedir.

Anahtar Kelimeler

Chalcalburnus tarichi, apoptoz, hepatosit kültürü, akım sitometri, 17α-Etinilestradiol, -n-Nonilfenol

Destekleyen Kurum

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

2007-FED-B42

Teşekkür

Bu çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından desteklenmiştir (Proje No: 2007-FED-B42). Desteğinden dolayı Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı’na teşekkür ederiz.

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