Evaluation of the Effect of Galanin and Exercise on Anxiety in Rats by Open Field and Elevated Plus Maze Tests

Year 2018, Volume: 20 Issue: 3, 63 - 68, 11.12.2018

Asuman Mermerci , Recep Özmerdivenli , Hayriye Orallar Ersin Beyazçiçek , Mehmet Ali Sungur

https://doi.org/10.18678/dtfd.490123

Cited By: 3

Abstract

Aim: Anxiety, known as worry, considered normal in everyday life, and also it is a type of behavior developed to protect the organism. The aim of this study is to investigate the effects of exogenous galanin given with exercise on anxiety.

Material and Methods: In this study 96 adult male Wistar Albino rats were used. Rats were divided into 8 groups with twelve animals, and 4 of the groups were given regular exercise for 6 weeks. Galanin was administered intraperitoneally (IP) and intracerebroventricular (ICV) in half of the groups and saline was injected into the control group of each group with the same methods. At the end of the sixth week, open field and elevated plus maze tests were applied to the rats, and these tests recorded with the camera. The recordings of the both tests were analyzed, and total distance and speed values, the time spent in the open field test and the time spent in the closed arm of elevated plus maze were compared.

Results: In the open field test were determined that in general, the total distance (p<0.001) and the speed increased (p<0.001) in rats injected with ICV compared to the rats injected with IP. There is no significant difference was detected in terms of exercise and galanin interaction. Also the total distance (p=0,032) and the speed increased (p=0,010) in rats injected with ICV compared to the rats injected with IP. In elevated plus maze test, exercise and galanin interaction were not found to be statistically significant, however the interaction between the injection pattern and the given substance was found to be significant in terms of time spent in the closed arm (p=0.020) and speed (p=0.032).

Conclusion: Although it has been shown that central administration of galanin may have anxiolytic activity, this effect has not changed with exercise, and also it has been determined that exercise with galanin has no effect on this activity.

Keywords

Exercise, galanin, anxiety, rat, open field test, elevated plus maze test

Galanin ve Egzersizin Ratlarda Anksiyete Üzerine Etkisinin Açık Alan ve Yükseltilmiş Artı Labirent Testleri ile Değerlendirilmesi

Abstract

Amaç: Anksiyete, kaygı olarak bilinen, günlük yaşamda normal kabul edilen ve organizmayı korumak amaçlı gelişen bir davranış türüdür. Bu çalışmanın amacı egzersizle birlikte verilen eksojen galaninin anksiyete üzerine etkilerinin araştırılmasıdır.

Gereç ve Yöntemler: Çalışmada 96 yetişkin erkek Wistar Albino rat kullanıldı. Ratlar on ikişerli 8 gruba ayrıldı ve 4 gruba 6 hafta boyunca düzenli egzersiz yaptırıldı. Grupların yarısına galanin intraperitonal (IP) ve intraserebroventriküler (ICV) şekilde verildi ve her grubun kendi kontrol grubuna da aynı yöntemlerle salin enjekte edildi. Altıncı hafta sonunda ratlara açık alan ve yükseltilmiş artı labirent testleri uygulanarak kamera kayıtları alındı. Her iki testin de kayıtları incelenerek elde edilen katedilen toplam mesafeler ve hız değerleri ile açık alan testinde merkezde geçirilen süre ve yükseltilmiş artı labirent testinde kapalı kolda geçirilen süre karşılaştırıldı.

Bulgular: Açık alan testinde, genel olarak ICV enjeksiyon yapılan ratlarda, IP enjeksiyon yapılan ratlara göre katedilen toplam mesafenin (p<0,001) ve hızın arttığı (p<0,001) tespit edilmiştir. Egzersiz ve galanin etkileşimi açısından bakıldığında da anlamlı bir farklılık tespit edilmemiş, yine ICV galanin verilen ratlarda IP galanin verilen ratlara kıyasla katedilen toplam mesafenin (p=0,032) ve hızın (p=0,010) arttığı görülmüştür. Yükseltilmiş artı labirent testinde de egzersiz ve galanin etkileşimi istatistiksel olarak önemli bulunmamış, enjeksiyon şekli ve verilen madde etkileşiminin ise kapalı kolda kalış süresi (p=0,020) ve hız (p=0,032) açısından anlamlı olduğu tespit edilmiştir.

Sonuç: Galaninin santral olarak verilmesinin anksiyolitik etkinliği olabileceği gösterilmekle birlikte bu etkinin egzersizle birlikte değişmediği, galanin ile birlikte egzersiz uygulamasının bu etkinlik üzerine herhangi bir etkisi olmadığı belirlenmiştir.

Keywords

Egzersiz, galanin, anksiyete, rat, açık alan testi, yükseltilmiş artı labirent testi

References

• Belzung C, Philippot P. Anxiety from a phylogenetic perspective: is there a qualitative difference between human and animal anxiety. Neural Plast. 2007; 2007:59676.
• Uzbay İT. Anksiyetenin nörobiyolojisi, Klinik Psikiyatri Dergisi 2002;(Ek Sayı 1):5- 13.
• DSM-IV-TR, A. P. A. Diagnostic and statistical manual of mental disorders, text revision. Washington, DC: American Psychiatric Association, 2000.
• Mendlowicz MV, Stein MBA. Quality of life in individuals with anxiety disorders. J Psychiatry. 2000; 157(5):669-82.
• Kessler RC, Aguilar-Gaxiola S, Alonso J, Chatterji S, Lee S, Ormel J, Wang PS. The global burden of mental disorders: an update from the WHO World Mental Health (WMH) surveys. Epidemiology and Psychiatric Sciences. 2009; 18(1), 23-33.
• American Psychiatric Association . Diagnostic and statistical manual of mental disorders. IVR Washington, D.C. 2000.
• Goodwin, Renee D. Association between physical activity and mental disorders among adults in the United States. Preventive medicine. 2003; 36.6: 698-703.
• Karim A-O, Rütten A, Lehtinen V. Mental health and physical activity in the European Union." Sozial-und Präventivmedizin. 2004;49.5: 301-309.
• Herring MP, O’connor PJ, Dishman RK. The effect of exercise training on anxiety symptoms among patients: a systematic review." Archives of internal medicine 170.4 (2010): 321-331.
• Carek PJ, Laibstain SE, Carek SMI. Exercise for the treatment of depression and anxiety. J Psychiatry Med. 2011; 41(1):15-28.
• Greenwood BN, Fleshner. Exercise, stress resistance, and central serotonergic systems. Exerc Sport Sci Rev. 2011; 39(3):140-9.
• Fuss J, Ben Abdallah NM, Hensley FW, Weber KJ, Hellweg R, Gass P. Deletion of running-induced hippocampal neurogenesis by irradiation prevents development of an anxious phenotype in mice. PLoS One. 2010; 5(9): e12769.
• Van Praag H. Exercise and the brain: something to chew on. Trends Neurosci. 2009; 32(5):283-90.
• Webling KEB, Runesson J, Bartfai, T, Langel Ü. (2012). Galanin receptors and ligands. Frontiers in endocrinology. 2012; 3, 146.
• Lang R, Gundlach AL, Kofler B. The galanin peptide family: Receptor pharmacology, pleiotropic biological actions, and implications in health and disease. Pharmacol. Ther. 2007; 115: 177–207.
• Corness J, Shi TJ, Xu ZQ, Brulet P, Hökfelt T. Influence of leukemia inhibitory factor on galanin/GMAP and neuropeptide Y expression in mouse primary sensory neurons after axotomy. Exp. Brain Res. 1996; 112:79–88.
• Holmes PV, Crawley JN. Coexisting neurotransmitters in central noradrenergic neurons. In: Bloom FE, Kupfer DJ, editors.Psychopharmacology: The Fourth Generation of Progress. Raven Press; New York: 1995. p. 347–353.
• Huang HP, Wang SR, Yao W, Zhang C, Zhou Y, Chen XW, et al. Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices. Proc Natl Acad Sci U S A. 2007; 104(4):1401-6.
• O'Neal HA, Van Hoomissen JD, Holmes PV, Dishman RK. Prepro-galanin messenger RNA levels are increased in rat locus coeruleus after treadmill exercise training. Neurosci Lett. 2001; 299(1-2):69-72.
• Uchiumi K, Aoki M, Kikusui T, Takeuchi Y, Mori Y. Wheel-running activity increases with social stress in male DBA mice. Physiol Behav. 2008; 93(1-2):1-7.
• Karlsson RM, Holmes A, Heilig M, Crawley JN. Anxiolytic-like actions of centrally-administered neuropeptide Y, but not galanin, in C57BL/6J mice. Pharmacol Biochem Behav. 2005 Mar; 80(3):427-36.
• Candland DK. The open field: some comperative data. Annals New York Academy of Sciences. 1969; 159:831-851.
• Prut I, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. European Journal of Pharmacology. 2003; 463:3-33.
• Phillips KM. Effects of time and administration of ethanol on open field behavior in hamsters. Physiology & Behavior. 1982; 29: 785-787.
• Lieben CKJ, Oorsouw K, Deutz NEP, Blokland A. Acute tryptophan depletion induced by a gelatin-based mixture impairs object memory but not affective behavior and spatial learning in the rat. Behavioural Brain Research. 2004;151:53-64.
• Binder E, Droste SK, Ohl F. Regular voluntary exercise reduces anxiety-related behaviour and impulsiveness in mice., Reul JMBehav Brain Res. 2004; 155(2):197-206.
• Hopkins ME, Bucci D. BDNF expression in perirhinal cortex is associated with exercise-induced improvement in object recognition memory. JNeurobiol Learn Mem. 2010;94(2):278-84.
• Grace L, Hescham S, Kellaway LA, Bugarith K, Russell VA. Effect of exercise on learning and memory in a rat model of developmental stress. Metab Brain Dis. 2009; 24(4):643-57.
• Pietropaolo S, Feldon J, Alleva E, Cirulli F, Yee BK. The role of voluntary exercise in enriched rearing: a behavioral analysis. Behav Neurosci. 2006; 120(4):787-803.
• Takahashi A, Nishi A, Ishii A, Shiroishi T, Koide T. Systematic analysis of emotionality in consomic mouse strains established from C57BL/6J and wild-derived MSM/Ms. Genes Brain Behav. 2008; 7(8):849-58.
• Stranahan AM, Lee K, Mattson MP. Central mechanisms of HPA axis regulation by voluntary exercise. Neuromolecular Med. 2008; 10(2):118-27.
• Rajarao SJ, Platt B, Sukoff SJ, Lin Q, Bender CN, Nieuwenhuijsen BW, et al. Anxiolytic-like activity of the non-selective galanin receptor agonist, galnon. Neuropeptides. 2007; 41(5):307-20.
• Lyudyno VI, Abdurasulova IN, Klimenko VM. The role of the neuropeptide galanin in forming type-specific behavioral characteristics. Neurosci Behav Physiol. 2008; 38(1):93-8.
• Swanson CJ, Blackburn TP, Zhang X, Zheng K, Xu ZQ, Hökfelt T, et al. Anxiolytic- and antidepressant-like profiles of the galanin-3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299. Proc Natl Acad Sci U S A. 2005; 102(48):17489-94.
• Rotzinger S, Lovejoy DA, Tan L. Behavioral effects of neuropeptides in rodent models of depression and anxiety. Peptides. 2010 Apr; 31(4):736-56.