Thiacloprid ve D-Tubokurarin’in Rana ridibunda Gastrokinemius Kası Üzerine Toksik Etkileri III: Oksidatif Potansiyel

Yıl 2016, Cilt: 12 Sayı: 2, 141 - 148, 01.11.2016

Yusuf Çamlıca , Esra Pekoğlu Serap Yalın

https://doi.org/10.22392/egirdir.285127

Öz

Bu çalışmada neonikotinoid bir insektisit olan thiacloprid ve antagonisti d-tubokurarin’in kurbağa
gastrokinemius kasında, tiyobarbitürik asit reaktif madde düzeyleri ve katalaz enzim aktivitesi üzerine etkileri
araştırılmıştır. Deneylerde 35 adet kurbağa kas preparatı kullanılmıştır. Gastrokinemius kası izole edildikten
sonra, 120 dakika boyunca 250, 25, 2,5 ve 0,25 mg/L olmak üzere thiacloprid’in 4 farklı konsantrasyonuna
maruz bırakılmıştır. Ayrıca, izole kaslar 2,5 mg/L thiacloprid ile 80 mg/L d-tubokurarin karışımı ve 0,25 mg/L
thiacloprid ile 8 mg/L d-tubokurarin karışımı ile muamele edilmiştir. Kontrol grubundaki kas dokuları ise, 120
dakika boyunca Ringer çözeltisi içerisinde bekletilmiştir. Her bir konsantrasyon grubu eşit sayıda preparat ile
çalışılmıştır (n=5).Yapılan incelemeler sonucunda 250, 25, 2,5 ve 0,25 mg/L thiacloprid, 0,25 mg/L
thiacloprid ile 8 mg/L d-tubokurarin karışımının çizgili kas dokusunda kontrol grubuna göre, tiyobarbitürik
asit reaktif madde düzeylerini arttırdığı gözlenmiştir (P<0,05). 250 (P<0,001) ve 25 mg/L thiacloprid
(P<0,05), kas dokuda katalaz enzim aktivitesini azaltmıştır. Bu çalışma, thiacloprid ve d-tubokurarin’in çizgili
kaslarda oksidatif stres oluşturma potansiyelinin, hedef olmayan organizmalara olası etkilerinin ve çevresel
risklerinin değerlendirilmesinde önemli bilgiler sunmaktadır. 

Anahtar Kelimeler

: Thiacloprid, d-tubokurarin, kurbağa, gastrokinemius

Kaynakça

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