Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes

Yıl 2018, Cilt: 1 Sayı: 2, 48 - 53, 20.12.2018

Muhammet Dogan , Bugrahan Emsen

Öz

Bacopa
monnieri L. Pennell is an
important medicinal plant with antioxidant and neuroprotective role. It acts as an efficient free radical scavenger and
has the potential to serve as a phytoremedy in the disease conditions involving
reactive oxygen species (ROS) and oxidative stress. In
addition, the protective role of B. monnieri on
tissue antioxidant defense system and lipid peroxidative status in diabetic
rats has been reported. B. monnneri
used in the experiment was propagated by plant tissue culture techniques. The
shoot tip explants of B. monnneri
were cultured in Murashige and Skoog (MS) nutrient medium containing 1.0 mg/L
6-Benzylaminopurine (BAP). Genotoxic effects of aqueous extract
obtained from B. monnieri in
cultured human lymphocytes were determined by
chromosome aberration (CA) test. In addition, cytotoxic activities of the
extract on the cells were revealed via
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis.
Different concentrations (6.25-200 mg/L) of the extract were used in the
treatments. It was detected that cytotoxicty was correlated with concentration.
Cell viability decreased to 69.64% in the lymphocytes exposed to maximum
concentration of the extract. Based on CA/cell test, the results were
statistically (p > 0.05)
indifferent from negative control treatment. Overall, obtained data indicated
that B. monnieri is a plant that
contains natural products that are not causative of cytotoxic and genotoxic
damage on human lymphocytes.

Anahtar Kelimeler

Cytotoxicity, Genotoxicity, Shoot Regeneration

Kaynakça

• Amoo SO, Aremu AO, Staden JV 2012. In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill. Plant Cell Tissue Organ Cult 111: 345-358.
• Ari F, Ulukaya E, Oran S, Celikler S, Ozturk S, Ozel MZ 2015. Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes. Cytotechnology 67: 531–543.
• Aydin E, Turkez H 2011a. Effects of lichenic extracts (Bryoria capillaris, Peltigera rufescens and Xanthoria elegans) on human blood cells: A cytogenetic and biochemical study. Fresenius Environ Bull 20: 2992–2998.
• Aydin E, Turkez H 2011b. Antioxidant and genotoxicity screening of aqueous extracts of four lichens collected from North East Anatolia. Fresenius Environ Bull 20: 2085–2091.
• Bairu MW, Amoo SO, Staden JV 2011. Comparative phytochemical analysis of wild and in vitro-derived greenhouse-grown tubers, in vitro shoots and callus-like basal tissues of Harpagophytum procumbens. S Afr J Bot 77: 479-484.
• Baskaran P, Moyo M, Staden JV 2014. In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum. S Afr J Bot 90: 74-79.
• Bayram S, Ecem Bayram N, Gerçek YC, Öz GC, Sorkun K 2016. Anticytotoxic and antimutagenic effects of propolis on human lymphocytes in vitro. Mellifera 16: 38–46.
• Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S 2000. Antioxidant activity of Bacopa monnieri in rat frontal cortex, striatum and hippocampus. Phytother Res 14: 174-179.
• Bourrain L 2018. In vitro propagation of Actinidia melanandra Franch. and Actinidia rubricaulis Dunn. from shoot tip explants. N Z J Crop Hortic Sci 46(2): 162-173.
• Danova K, Cellárová E, Macková A, Daxnerová Z, Kapchina-Toteva V 2010. In vitro culture of Hypericum rumeliacum Boiss and production of phenolics and flavonoids. In Vitro Cell Dev Biol 46: 422-429.
• Dogan M, Karatas M, Aasim M 2015. An efficient in vitro plantlet regeneration of Ceratophyllum demersum L., an important medicinal aquatic plant. Fresen Environ Bull 24(10b): 3499-3504.
• Dogan M 2017. Multiple shoot regeneration from shoot tip and nodal explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot 1(1,2): 4-8.
• Dogan M 2018a. Tıbbi bitki Lysimachia nummularia L.'nın boğum eksplantlarından in vitro mikroçoğaltımı. KSÜ Tarım ve Doğa Dergisi, 21(6): 875-881.
• Dogan M 2018b. Limnophila aromatica (Lamk.) Merr.'nın boğum ve boğum arası eksplantlarından in vitro sürgün rejenerasyonu. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 8(3): 77-84.
• Emsen B, Turkez H, Togar B, Aslan A 2017. Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts. Hum Exp Toxicol 36: 376–385.
• Emsen B, Dogan M 2018. Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Sci Pol Cultus 17: 23–33.
• Emsen B, Aslan A, Kaya A 2018a. Biological activities of Platismatia glauca (L.) W.L.Culb. & C.F.Culb. on human lymphocytes. Süleyman Demirel Univ J Nat Appl Sci 22: 840–848.
• Emsen B, Aslan A, Kaya A 2018b. Cytotoxic, genotoxic and oxidative effects of Cladonia furcata (Huds.) Schrad. on human peripheral lymphocytes. Cumhur Sci J 39: 169–180.
• Emsen B, Togar B, Turkez H, Aslan A 2018c. Effects of two lichen acids isolated from Pseudevernia furfuracea (L.) Zopf in cultured human lymphocytes. Zeitschrift fur Naturforsch Sect C-A J Biosci 73: 303–312.
• Feng L, Huang Q, Huang Z, Li H, Qi X, Wang Y, Liu Z, Liu X, Lu L 2016. Optimized animal model of cyclophosphamide-induced bone marrow suppression. Basic Clin Pharmacol Toxicol 119: 428–435.
• Gadano AB, Gurni AA, Carballo MA 2006. Argentine folk medicine: genotoxic effects of Chenopodiaceae family. J Ethnopharmacol 103: 246–251.
• Geyikoglu F, Turkez H 2008. Boron compounds reduce vanadium tetraoxide genotoxicity in human lymphocytes. Environ Toxicol Pharmacol 26: 342–347.
• Gnanaraj WE, Marimuthu J, Subramanian KM, Nallyan S 2011. Micropropagation of Alternanthera sessilis (L.) using shoot tip and nodal segments. Iran J Biotechnol 9: 206-212.
• Imai Y, Ishida K, Nemoto M, Nakata K, Kato T, Maéno M 2017. Multiple origins of embryonic and tadpole myeloid cells in Xenopus laevis. Cell Tissue Res 369: 341–352.
• Jafarpour SM, Safaei M, Mohseni M, Salimian M, Aliasgharzadeh A, Fahood B 2018. The radioprotective effects of curcumin and trehalose against genetic damage caused by I-131. Indian J Nucl Med 33: 99–104.
• Karatas M, Dogan M, Emsen B, Aasim M 2015. Determination of in vitro free radical scavenging activities of various extracts from in vitro propagated Ceratophyllum demersum L. Fresenius Environ Bull 24: 2946–2952.
• Kashyap S, Kapoor N, Kale RD 2018. Micropropagation of B. monnieri using humin media in plant tissue culture. Ann Plant Sci 6(5): 1625-1629.
• Kazmin SD, Todor IN, Chekhun VF 2005. Antitumor resistance activation in mice: Can the immunological memory cells enhance resistance? J Exp Clin Cancer Res 24: 585–593.
• Lin X-F, Luo J-W, Gui LIU, Zhu Y-B, Zhao JIN, Xing LIN 2018. Genetic mutation of familial dilated cardiomyopathy based on next-generation semiconductor sequencing. Mol Med Rep 18: 4271–4280.
• Łukasiewicz K, Fol M 2018. Microorganisms in the treatment of cancer: advantages and limitations. J Immunol Res 2018: 2397808.
• Luu J, Palczewski K 2018. Human aging and disease: lessons from age-related macular degeneration. Proc Natl Acad Sci U S A 115: 2866–2872.
• Mathew J, Paul J, Nandhu MS, Paulose CS, 2010. Bacopa monnieri and Bacoside-A for ameliorating epilepsy associated behavioral deficits. Fitoterapia 81: 315-322.
• Mäurer M, Kobsar I, Berghoff M, Schmid CD, Carenini S, Martini R 2002. Role of immune cells in animal models for inherited neuropathies: facts and visions. J Anat 200: 405–414.
• Murashige T, Skoog F 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473-497.
• Naik PM, Godbole M, Nagella P, Murthy HN 2017. Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in Bacopa monnieri (L.). Ceylon J Sci 46(4): 97-104.
• Rastogi S, Pal R, Kulshreshtha DK 1994. Bacoside A3-a triterpenoid saponin from Bacopa monniera. Phytochemistry 36: 133-137.
• Rodríguez-Ribera L, Pastor S, Corredor Z, Silva I, Diaz JM, Ballarin J, Marcos R, Coll E 2016. Genetic damage in patients moving from hemodialysis to online hemodiafiltration. Mutagenesis 31: 131–135.
• Roodenrys S, Booth D, Bulzomi S, Phipps A, Micallef C, Smoker J 2002. Chronic effects of Brahmi (Bacopa monnieri) on human memory. Neuropsychopharmacology 27: 279-281.
• Ruehl-Fehlert C, Parker GA, Elmore SA, Kuper CF 2017. Immune System. In: Fundamentals of Toxicologic Pathology: Third Edition. pp. 273–313.
• Russo A, Borrelli F 2005. Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine 12: 305-317.
• Sharma S, Kamal B, Rathi N, Chauhan S, Jadon V, Vats N, Gehlot A, Arya S 2010. In vitro rapid and mass multiplication of highly valuable medicinal plant Bacopa monnieri (L.) Wettst. Afr J Biotechnol 9(49): 8318-8322.
• Showkat P, Zaidi Y, Asghar S, Jamaluddin S. 2010. In vitro propagation and callus formation of Bacopa monnieri (L.) Penn. Plant Tissue Cult & Biotech 20(2): 119-125.
• Shrivastava P, Tiwari KN, Srivastava G 2017. Effect of different carbon sources on in vitro regeneration of Brahmi Bacopa monnieri (L.) An important memory vitalizer. J Med Plant Res 5(3): 202-208.
• Turkez H, Tatar A, Hacimuftuoglu A, Ozdemir E 2010. Boric acid as a protector against paclitaxel genotoxicity. Acta Biochim Pol 57: 95–97.
• Turkez H 2011. The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests. Exp Toxicol Pathol 63: 453–457.
• Turkez H, Aydin E 2013. The genoprotective activity of resveratrol on permethrin-induced genotoxic damage in cultured human lymphocytes. Brazilian Arch Biol Technol 56: 405–411.
• Turkez H, Celik K, Togar B 2014. Effects of copaene, a tricyclic sesquiterpene, on human lymphocytes cells in vitro. Cytotechnology 66: 597–603.
• Uabundit N, Wattanathorn J, Mucimapura S, Ingkaninan K 2010. Cognitive enhancement and neuroprotective effects of Bacopa monnieri in Alzheimer's disease model. J Ethnopharmacol 127(1): 26-31.
• Valdez H, Connick E, Smith KY, Lederman MM, Bosch RJ, Kim RS, St. Clair M, Kuritzkes DR, Kessler H, Fox L, Blanchard-Vargas M, Landay A 2002. Limited immune restoration after 3 years’ suppression of HIV-1 replication in patients with moderately advanced disease. AIDS 16: 1859–1866.
• Vijaykumar M, Vijaykumar R, Stephen R 2010 In vitro propagation of Bacopa monnieri L.-A multipurpose medicinal plant. ndian J Sci Technol 3(7): 781-786.
• Wiesner DL, Smith KD, Kashem SW, Bohjanen PR, Nielsen K 2017. Different lymphocyte populations direct dichotomous eosinophil or neutrophil responses to pulmonary cryptococcus infection. J Immunol 198: 1627–1637.
• Zumla AI, James DG 2002. Immunologic aspects of tropical lung disease. Clin Chest Med 23: 283–308.