Investigation of Slc30a8 (Rs13266634) Gene Polymorphisms in Type 2 Diabetes Mellitus Patients

Year 2023, Volume: 2 Issue: 2, 6 - 13, 25.10.2023

Burcu Aişeoğlu , Arif Parmaksız , Dilara Ulusal

Abstract

Type 2 Diabetes Mellitus (T2DM), a complex metabolic condition, is caused by a number of environmental and genetic factors, as well as their interactions. Many genes associated with T2DM have been discovered through genome-wide association studies. One of these genes is the SLC30A8 gene. T2DM and the SLC30A8 gene are linked to zinc, which is required for insulin secretion and storage. The aim of the research was to look into the relationship between T2DM disease and the rs13266634 polymorphism in the SLC30A8 gene. The research included 80 healthy people and 80 people with type 2 diabetes who were chosen at random from Şanlıurfa. A commercial kit was used to isolate DNA. Following isolation, the SLC30A8 gene region was amplified using PCR and cut with the HpaII enzyme. TT, CT, and CC alleles were discovered after cutting. We used data from 45 diagnosed T2DM cases and 52 confirmed healthy control subjects to detect alleles, and the gel imaging results were reliable and confident. The data analysis was evaluated using the SPSS method. The findings revealed a statistically significant relationship between the patient and control groups and the rs13266634 polymorphism in the SLC30A8 gene. Clarifying the genetic relationship between the rs13266634 polymorphism in the SLC30A8 gene and T2DM disease may aid in identifying genetic predispositions and developing risk profiles.

Keywords

Diabetes mellitus, Type 2 diabetes, polymorphism, SLC30A8 (rs13266634)

Supporting Institution

Harran University Research Fund

Project Number

22022

Thanks

For their significant contributions to our work, we would like to thank Assoc. Dr. to Özlem ÖZ, the Faculty Member to Çiğdem Cindioğlu and Fehime Dağ. This study was funded by Harran University Research Fund (Project No:22022)

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