In vitro Propagation of Micromeria dolichodontha P.H. Davis via Axillary Shoot Proliferation

Yıl 2018, Sayı: 14, 337 - 342, 31.12.2018

Yelda Emek

https://doi.org/10.31590/ejosat.492321

Cited By: 2

Öz

The goal of this paper was to determine the protocol for in vitro axillary shoot propagation of Micromeria dolichodontha P.H. Davis, an endemic endangered
and medicinal plant. Plantlets obtained from in vitro germinated seeds
were used as explants for axillary shoot propagation. The plantlets were separated from their roots
and cultured on Murashige & Skoog (MS) media wihout and with different
types and concentrations of cytokinins (BA, KIN or TDZ). The maximum number of
shoots was formed on MS medium with 0.1 mgL^-1  benzyladenine (BA), and the highest seedling
length was formed on MS medium with 0.01 mgL^-1 BA. ½ MS medium
wihout and with different types and concentrations of auxin (IBA or IAA) were
used for rooting of shoots. The maximum root formation (75 %) were observed on
½ MS medium with 0.5 mgL^-1 IBA.

Anahtar Kelimeler

Endemic, Micromeria dolichodontha, in vitro, axillary shoots

Micromeria dolichodontha P.H. Davis 'nın In vitro Aksiller Sürgün Yoluyla Çoğaltımı

Öz

Bu
çalışmanın amacı endemik, nesli kritik tehlikede olan ve tıbbi Micromeria
dolichodontha P.H. Davis türünün
in vitro aksiller sürgün yoluyla çoğaltma protokolünü belirlemektir. In vitro şartlar altında tohumların
çimlendirilmesi ile elde edilen bitkicikler aksiller sürgün çoğaltımı için eksplant
olarak kullanılmışlardır. Bitkicikler köklerinden ayrılarak farklı tip ve
konsantrasyonda sitokinin (BA, KIN veya TDZ) içeren ve kontrol olarak herhangi
bir bitki büyüme düzenleyicisi içermeyen Murashige & Skoog (MS) besin ortamlarında
kültüre alınmışlardır. En yüksek sürgün sayısı 0.1 mgL^-1 benziladenine (BA)
içeren MS ortamında ve en uzun sürgünler 0.01 mgL^-1 BA
içeren MS ortamında oluşmuştur. Sürgünlerin köklendirilmesi için farklı tip ve
konsantrasyonda oksin (IBA veya IAA) içeren ve herhangi bir bitki büyüme
düzenleyicisi içermeyen ½ MS ortamı kullanılmıştır. En yüksek kök oluşumu (% 75)
0.5
mgL^-1 IBA içeren ½ MS ortamında gözlenmiştir.

Anahtar Kelimeler

Endemik, Micromeria dolichodontha, in vitro, aksiller sürgünler

Kaynakça

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