Dorystoechas hastata 'nın Kallus Kültürlerinin Oluşturulmasında Eksplant Tipi ve Bitki Büyüme Düzenleyicilerinin Etkisi

Yıl 2019, Sayı: 16, 367 - 373, 31.08.2019

Bengi Erdağ , Yelda Emek , Serap Kurt Aydoğan

https://doi.org/10.31590/ejosat.554947

Öz

Bu çalışmanın amacı monotipik endemik Dorystoechas hastata bitkisinin kallus kültürlerinin oluşturulmasında eksplant tipi ve bitki büyüme düzenleyicilerinin etkisinin belirlemektir. Doğal ve in vitro elde edilmiş bitkilerin çeşitli kısımları tek başına BA veya 2,4-D ve kombinasyonlarını içeren ve hiçbir bitki büyume düzenleyici içermeyen 1/2 MS besi ortamlarına alınarak uygun eksplant tipi ve büyüme düzenleyici konsantrasyonu belirlenmiştir. Doğal ortamından toplanan yaprak, yaprak sapı ve in vitro fidelerden elde edilen yaprak sapı eksplantları kültüre cevap vermemiştir. In vitro fidelerden elde edilen yaprak eksplantları sadece 2 mgL-1 BA içeren 1/2 MS ortamında kallus oluşturmuşlardır. Kotiledon eksplantları ise kültüre farklı oranlarda kallus indüksiyon frekansı oluşturarak cevap vermişlerdir. En yüksek kallus indüksiyon frekansı 1 mgL-1 BA ve 2 mgL-1 2,4-D içeren 1/2 MS ortamında elde edilmiştir (% 55). Dorystoechas hastata bitkisinin kallus kültürlerinin oluşturulmasında kotiledonlar en uygun eksplant olarak belirlenmiştir

Anahtar Kelimeler

Dorystoechas hastata, kallus, kotiledon, bitkibüyümedüzenleyicisi

Destekleyen Kurum

Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

FEF-06014

Teşekkür

Bu çalışmanın gerçekleştirilmesi için tohum temin eden Doç. Dr. Özkan Eren’e ve finansal destek sağlayan Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimine (Proje no: FEF-06014) teşekkür ederiz. Bu çalışmanın bir kısmı, 23-27 Haziran 2008 tarihleri arasında Trabzon-Türkiye de düzenlenen 19. Ulusal Biyoloji Kongresi'nde poster (PB 284) olarak sunulmuştur.

Development of Callus due to Explant Type and Plant Growth Regulators in Dorystoechas hastata Plant

Öz

This study was planned to determine the effect of explant type and plant growth regulators in the formation of callus cultures of the monotypic endemic Dorystoechas hastata plant. Various parts of the plants collected from their natural population and obtained in vitro were cultured on 1/2 MS medium containing BA or 2,4-D alone and combinations of these growth regulators. 1/2 MS medium without plant growth regulators was determined as control medium. The explants collected from wild and petiol explants obtained from in vitro seedlings did not respond to culture. Leaf explants obtained from in vitro seedlings formed calli only on 1/2 MS medium with 2 mgL-1 BA. Cotyledon explants obtained from in vitro seedlings were responded to the culture by creating callus induction frequency at different rates. The highest callus induction frequency was obtained on 1/2 MS medium with 1 mgL-1 BA and 2 mgL-1 2,4-D (55%). At the end of the experiments, cotyledons were determined as the most suitable explants in the formation of callus cultures of Dorystoechas hastata.

Anahtar Kelimeler

Dorystoechas hastata, callus, cotyledon, plant growth regulators

Proje Numarası

FEF-06014

Kaynakça

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