Yüksek saponin içerikli Saponaria officinalis L. ile Sentezlenen Bakır Nanopartiküllerin Karakterizasyonu ve Antibakteriyel Aktivitesi

Yıl 2022, Sayı: 35, 341 - 348, 07.05.2022

Hamdi Kamçı , Recep Taş , Hasan Ufuk Celebioglu

https://doi.org/10.31590/ejosat.1063095

Cited By: 1

Öz

Metalik nanoparçacıklar genellikle sentezlendikleri metal iyonlarından daha kararlı ve aktiftir ve hem nanoparçacıklar hem de metal iyonları antimikrobiyal özelliklere sahiptir. Antimikrobiyal etkileri genellikle mantarlarla birlikte gram pozitif, gram negatif bakterileri kapsar. Spesifik temel sentetik yolları hedefleyen antibiyotiklerin aksine metalik nanopartiküllerin spesifik etki mekkanizmaları yoktur. Bu nedenle antibakteriyel özelliklere sahip NP'ler, çoklu ilaç direnci gösteren inatçı bakteriyel enfeksiyonlara karşı potansiyel ajanlar olarak öngörülmektedir.
Bu çalışmada yeşil yoldan bakır nanoparçacık (CuNP) sentezi, yapısal karakterizasyonu ve antibakteriyel özelliklerinin değerlendirilmesi rapor edilmiştir. Seçilen indirgeyici madde kaynağı, saponin içeriği yüksek olduğu bilinen çöğen (Saponaria officinalis L.) kök ekstreleridir. Bakır ve S. officinalis kök ekstraktlarından yeşil nanopartiküller üretmekteki temel amaç, hem CuNP'lerin hem de sabun otundan elde edilen biyoaktif fitokimyasalların antibakteriyel etkilerini birleştirmektir.
Bu amaçal sentezlenen Yeşil-CuNP'lerin yapısal karakterizasyonu, taramalı elektron mikroskobu (SEM), UV-Vis spektroskopisi (UV-Vis), fourier dönüşümlü kızılötesi spektrofotometre (FTIR) ve X-ışını kırınımı (XRD) analizi yoluyla gerçekleştirilmiştir. Muhtemelen saponinler ve diğer aktif fitokimyasallarla kaplanmış Yeşil-CuNP'lerin, iki yaygın bakteriye (Eschericia coli ve Staphylococus aeurous) karşı antimikrobiyal aktiviteleri test edilmiştir.
Üretilen NP'nin büyük kısmının, ortalama çapı 17 nm olan saf nanokristal yapılar olduğu (XRD verisi) gösterilmiş, FTIR analiz verilerinden NP’lerin, hem CuNP yapıda olduğu hem de aromatik hidrokarbon yapıları (muhtemelen saponinler) ile işlevselleştirildiği doğrulanmıştır. Antimikrobiyal özelliklerin değerlendirilmesinde ise NP’lerin yüksek dozlarda kullanıldığında E. coli ve S. aeurous'a karşı zayıf ila orta derecede ancak istatistiki olarak önemli antimikrobiyal etki gösterdiği görülmüştür.
S. officinalis ile sentezlenen Yeşil-CuNP'lerin antimikrobiyal aktivitesinin gerçek mekaniğini ortaya çıkarmak için daha fazla teşhis ve deney yapılabilir.

Anahtar Kelimeler

CuNPs, Saponaria officinalis, antibakteriyel, yeşil sentez.

Destekleyen Kurum

Bartın Üniversitesi

Characterization and Antibacterial Activity of Green Copper Nanoparticles Synthesized by Saponaria officinalis L., a plant with high saponin content

Öz

Metallic nanoparticles are generally more stable and active than the metal ions from which they were synthesized where both nanoparticles and metal ions usually have antimicrobial properties. Their antimicrobial effect generally covers gram-positive, gram-negative bacteria along with fungi. And unlike antibiotics that target specific essential synthetic pathways in the cell antimicrobial actions of metallic nanoparticles are nonspecific. Due to this fact NPs with antibacterial properties are foresen as potential agents against stubborn bacterial infections that show multidrug resistance.
In this work we report copper nanoparticle (CuNP) synthesis through green route, structural characterization and evaluation of its’ antibacterial properties. Source of reducing agent selected was soapwort (Saponaria officinalis L.) root extracts that are known to be high in saponin content. Main aim of generating green nanoparticles from copper and S. officinalis root extracts was to combine antibacterial effects of both CuNPs and bioactive phytochemicals from soapwort.
Structural characterisation of the Green-CuNPs were performed through scanning electron microscopy (SEM), UV-Visible spectroscopy (UV-Vis), fourier conversion infrared spectrophotometer (FTIR) and X-ray diffraction (XRD) analysis. Green-CuNPs coated (perhaps) with saponins and other active phytochemicals were tested for their antimicrobial activities against two common bacteria (Eschericia coli and Staphylococus aeurous).
The bulk of the genrated NP were shown to be pure nanocrystalline structures (XRD analysis) with average diameter of 17 nm. FTIR analysis data confirmed both CuNP formation and functionalization with aromatic hydrocarbon structures (possibly saponins). In the evaluation of antimicrobial properties, it was observed that NPs had weak to moderate but statistically significant antimicrobial effects against E. coli and S. aeurous when used at high doses.
Further diagnostics and experimentations may be done to reveal the true mechanistics of antimicrobial activity of Green-CuNPs synthessised with S. officinalis.

Anahtar Kelimeler

CuNPs, Saponaria officinalis, antibacterial, green synthesis.

Kaynakça

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