PEG-DA/Conconavalin A Biyokompozit Hidrojel Sentezi ve Kanser Tedavisi İçin Araştırılması

Yıl 2022, Sayı: 38, 406 - 414, 31.08.2022

Sabiha Demirci , Fatma Ayhan

https://doi.org/10.31590/ejosat.1125077

Öz

Kanser tedavisinde ilaç terapisi önemli bir araştırma alanını oluşturmaktadır. Conconavalin A(ConA) kanser hücreleri ile etkileşime girebildiği için anti-kanser ilaç çalışmalarında kullanılan bir lektindir. Bu çalışmada, ConA ve polietilen glikol diakrilat (PEGDA) kullanılarak hidrojel sentezi yapılmıştır. Sentezlenen hidrojellerin Fourier dönüşümlü infrared spektroskopisi ( FTIR) ve Taramalı Elektron mikroskobu (SEM) ile karakterizasyonu yapılmıştır. Hidrojellerin karakterizasyonunu incelediğimizde, PEGDA/ConA’nın birbirine entegre olduğu, biyokompozitlerin sentezlendiği gözlenmiştir. Sentezlenen hidrojellerin pH=7,4 ve 1,2’de dinamik şişme davranışları incelenmiştir. Sentezlenen PEGDA/ConA biyokompozit hidrojellerin şişme davranışlarını incelediğimizde yaklaşık 60 dakidada maksimuma ulaştığı gözlenmiştir. PEGDA/ConA hidrojellere geniş spektrumlu bir antibiyotik olan gentamisin yüklenmiş ve gentamisin yüklü hidrojellerin pH=7,4 ve pH=1,2’de ilaç salım davranışları incelenerek, karşılaştırılmıştır. PEGDA/ConA biyokompozit hidrojellerin pH=1,2 ilaç salım davranışlarına bakıldığında 400 dakikada ilaç salımının %15-20 civarında olduğu, pH=7,4’te ise 400 dakikada ilaç salımının %60’larda görülmüştür. Uzun süreli salım çalışmalarında kullanılabilecek ilaç salım sistemi olarak tasarımının uygun olduğu görülmüştür. Böylece anti-kanser ilaç salımında PEGDA/ConA hidrojellerin kullanılabileceği görülmüştür.

Anahtar Kelimeler

PEGDA, Conconavalin A, Biyokompozit, Hidrojel, Kanser Tedavisi

PEG-DA/Concanavalin A Biocomposite Hydrogels Synthesis And Investigations For Cancer Therapy

Öz

Drug therapy is an important research area in cancer treatment. Conconavalin a (ConA) is a lectin used for anti-cancer drug research because it can interact with cancer cells. In this study, hydrogels was synthesized using ConA and polyethylene glycol diacrylate (PEGDA). Synthesized hydrogels were characterized by Fourier-Transform Infrared Spectroscopy (FTIR) and the Scanning Electron microscope (SEM). When we examined at the characterization of hydrogels, it was observed that PEGDA/ConA was integrated and that biocomposites were synthesized. Dynamic swelling behavior of synthesized hydrogels were studied at pH = 7.4 and 1.2. When we examined the swelling behavior of synthesized PEGDA/ConA hydrogels, it was observed that biocomposite hydrogels reached maximum in approximately 60 minutes. PEGDA/ConA hydrogels were loaded gentamisin, a broad spectrum antibiotic,and drug release behavior of gentamicin loaded hydrogels at pH=7.4 and pH=1.2 were investigated and compared. Considering the drug release behavior of PEGDA/ConA biocomposite hydrogels at pH=1,2, it was observed that the drug release was around 15-20% in 400 minutes, and that the drug release was around 60% in 400 minutes at pH=7,4. It has been found that its design is suitable as a drug delivery system that can be used in long-term release studies. Thus, it has been seen that PEGDA/ConA hydrogels can be used in anti-cancer drug release.

Anahtar Kelimeler

PEGDA, Conconavalin A, Biocomposite, Hydrogels, Cancer Therapy

Kaynakça

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