Aydın İlinde Tüketime Sunulan Marullarda E. coli O157:H7 Varlığının Araştırılması

Yıl 2022, Sayı: 38, 507 - 513, 31.08.2022

Nalan Turgut , Osman Kaya

https://doi.org/10.31590/ejosat.1141099

Öz

Bu çalışmada Aydın ilinde bulunan semt pazarlarından ve marketlerden alınan marul örneklerinde ilk kez Escherichia coli O157:H7 varlığı araştırılmış, izolasyon ve identifikasyon çalışmaları yapılmıştır. Topum sağlığı açısından önemi olan E. coli O157:H7’nin gıda kaynaklı patojen mikroorganizmalardan birisi olması sebebiyle, söz konusu patojenin yaygınlığı ve kontaminasyon durumu ile benzer iklim özelliklerine sahip olan bölgeler için önemli bir veri kaynağı oluşturulması amaçlanmıştır. Çalışma kapsamında semt pazarlarından ve marketlerden alınan toplam 100 adet marul örneklerinin izolasyon ve identifikasyon çalışmaları sonucunda örneklerin tümünden toplam 17 (%17) adet E. coli O157:H7 izole ve identifiye edilmiş, patojenlerin 12 (%12) adedi pazarlardan, 5 (%5) adedi ise marketlerden alınan örneklerden elde edilmiştir. Elde edilen sonuçlar doğrultusunda pazar ve marketlerden satın alınan marul örnekleri E. coli O157:H7 varlığı açısından risk teşkil ederken konu ile ilgili olarak yetiştirme, gübreleme, sulama, taşıma ve depolama aşamalarında bulaşmayı engelleyici önlemlerin alınması gerektiği konusu vurgulanmıştır.

Anahtar Kelimeler

e.coli o157 h7, marul, kontaminsyon

Destekleyen Kurum

Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

VTF-14011

Teşekkür

Bu çalışma Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri VTF-14011 kodlu proje tarafından desteklenmiştir.

Investigation of E. coli O157:H7 Presence in Lettuce Served for Consumption in Aydın Province

Öz

In this study, the presence of Escherichia coli O157:H7 was investigated for the first time in lettuce samples taken from the neighborhood markets and markets in Aydın, and isolation and identification studies were carried out. Since E. coli O157:H7, which is important for public health, is one of the food-borne pathogenic microorganisms, it is aimed to create an important data source for regions with similar climatic characteristics with the prevalence and contamination status of the pathogen. As a result of isolation and identification studies of 100 lettuce samples taken from neighborhood markets and markets, a total of 17 (17%) E. coli O157:H7 samples were isolated and identified from all samples, 12 (12%) of pathogens were from markets, 5 (5%) were were obtained from samples taken from the markets. In line with the results obtained, it was stated that while the lettuce purchased from markets and markets poses a risk in terms of the presence of E. coli O157:H7, it is stated that preventive measures should be taken in the stages of cultivation, fertilization, irrigation, transportation and storage.

Anahtar Kelimeler

e.coli o 157h7, lettuce, contamination

Proje Numarası

VTF-14011

Kaynakça

• Al-Kharousi, Z.S, Guizani. N., Al-Sadi, A.M., Al-Bulushi, I.M., Shaharoona, B. (2016). Hiding in fresh fruits and vegetables: Opportunistic pathogens may cross geographical barriers. Hindawi Publishing Corporation International Journal of Microbiology, Article ID 4292417, 14.
• Berry, E.D, Wells, J.E., Durso, L.M., Friesen, K.M, Bono, J.L, Suslow, T.V. (2019). Occurrence of Escherichia coli O157:H7 in pest flies captured in leafy greens plots grown near a beef cattle feedlot. J. Food Prot, 82, 1300– 1307.
• Bozkurt, H., Bell, T., Ogtrop, F., Kim-YenPhan-ThieN, McConchie, R. (2021). Assessment of microbial risk during Australian industrial practices for Escherichia coli O157:H7 in fresh cut-cos lettuce: A stochastic quantitative approach. Food Microbiology, 95, 103691.
• Cayalvizhi, B.S., Balachandhar, D. (2018). Prevalence of Shiga-like toxin producing Escherichia coli strain (E. coli O157) in
• freshly consumed vegetables and its characterization. Journal of Food Safety, 39(1).
• Chapman, P.A., Siddons, A., Wright, D.J, Norman, P., Fox, J., Crick, E, (1993). Cattle as a possible source of verocytotoxin-producing Escherichia coli O157:H7 infections in man. Epidemiology of Infection, 111, 439-447.
• Chaudry, M.A., Bibi, N., Khan, M., Badshah. A., Qureshi, M.J., (2004). Irradiation treatment of minimally processed carrots for ensuring microbiological safety. Radiation Physics and Chemistry, 71, 169–173.
• Cui, Y., Walcott, R., Chen, J. (2017). Differential attachment of Salmonella enterica and enterohemorrhagic Escherichia coli to alfalfa, fenugreek, lettuce, and tomato seeds. Appl. Environ. Microbiol, 83(7), 1-7.
• De Jesus, A.J, Olsen, A.R, Bryce, J.R., Whiting, R.C. (20049. Quantitative contamination and transfer of Escherichia coli from foods by houseflies, Musca domestica L. (Diptera: Muscidae). Int. J. Food Microbiol, 93, 259– 262.
• Doyle, M.P., Schoeni, J.L. (1987). Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. Applied Environmental Microbiology, 53 (10), 2394-2396.
• Gündüz, T.G. (2008). Engelleme Teknolojisinin Sebzelerde Patojen İnaktivasyonu ve Raf Ömrü Açısından Etkilerinin Araştırılması. Doktora Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, Gıda Mühendisliği Anabilim Dalı, İzmir.
• Holvoet. K., Sampers, I., Seynnaeve, M., Jacxsen, L., Uyttendaele, M. (2015). Agricultural and management practices and bacterial contamination in greenhouse versus open field lettuce production. Int. J. Environ. Res. Public Health, 12(1), 32-63.
• Johannessen, G.S., Loncarevic, S., Kruse, H. (2002). Bacteriological analysis of fresh produce in Norway. International Journal of Food Microbiology, 77, 199-204.
• Kara, R., Acaröz, U., Gürler, Z., Soylu, A., Küçükkurt, O. (2019). Taze marul örneklerinde Escherichia coli O157 ve Listeria monocytogenes varlığının belirlenmesi. Avrupa Bilim ve Teknoloji Dergisi, 16, 871-873.
• Karp, D.S., Gennet, S., Kilonzo, C., Partyka, M., Chaumont, N., Atwill, E.R, Kremen, C. (2015). Comanaging fresh produce for nature conservation and food safety. Proc. Natl. Acad. Sci. USA, 112, 11126-1131.
• Koneman, E.W., Allen, S.D., Janda, W.M, Schreckenberger, P.C., Winn, W.C. (1997). Color atlas and textbook of diagnostic microbiology (fifth edition), Eds Andrew Allen, Lippincott-Raven Publishers, Philadelphia, 1307-1371.
• March, S.B., Ratnam, S.J. (1989). Latex agglutination test for detection of Escherichia coli serotype O157. Journal of Clinical Microbiology, 27(7), 1675–1677.
• Meng, J., LeJeune, J.T, Zhao, T, Doyle, M.P. (2012). Enterohemorajik Escherichia coli. Chapter 12.
• Mukherjee, A., Speh, D., Dyck, E., Diez-Gonzales, F. (2004). Preharvest evaluation of coliforms, Escherichia coli, Salmonella And Escherichia coli O157:H7 in organic and conventional produce grown by Minnesota farmers. Journal of Food Protection, 67, 894–900.
• Mukherjee, A, Cho, S., Scheftel, J., Jawahir, S., Smith and Diez-Gonzalez F. (2006). Soil survival of Escherichia coli O157 H7 acquired by a child from garden soil recently with cattle manure. Journal of Applied Microbiology, 101, 429-436.
• Öğüt, S., Polat, M. (2009). Bazı Beş Yıldızlı Otellerde Hazırlanan Gıdaların Mikrobiyolojik Açıdan Değerlendirilmesi. Süleyman Demirel Üniversitesi Yaşam Dergisi, 12-16.
• Sakazaki, R. (1992). Serotyping of diarrheagenic E. coli. Media Circle, 34, 117.
• Seow, J., Agoston, R., Phus, L., Yuk, H. (2012). Microbiological quality of fresh vegetables and fruits sold in Singapore. Food Control, 25, 39-44.
• Solomon, E., Yaron, S., Matthews, K. (2002). Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization. Applied Environmental Microbiology, 68, 397– 400.
• Szabo, R.A., Todd, E.C.D., Jean, A. (1986). A method to isolate Escherichia coli O157:H7 from food. Journal of Food Protection, 49: 768-772.
• Tian, P., Yang, D., Shan, L., Wang, D., Li, Q., Gorski, L., Lee, B.G., Quinones, B., Cooley, M.B. (2017). Concurrent detection of human norovirus and bacterial pathogens in water samples from an agricultural region in central California coast. Front. Microbiol, 8 (1560).
• Ustaçelebi, Ş. (1999). Temel ve Klinik Mikrobiyoloji. Ankara. Öncü Basımevi, 470-485.
• Van der Linden, I., Cottyn, B., Uyttendaele, M., Vlaemynck, G., Maes, M., Heyndrickx, M. (2013). Long-term survival of Escherichia coli O157:H7 and Salmonella enterica on butterhead lettuce seeds, and their subsequent survival and growth on the seedlings. Int. J. Food Microbiol, 161, 214– 219.
• Vernozy-Rozand, C. (1997). Detection of Escherichia coli O157:H7 and other Verocytotoxinproducing E. coli (VTEC) in food. Journal of Applied Microbiology, 82,537-551.
• Weagant, S.D, Bryant, J.L, Jinneman, K.G. (1995). An improved rapid technique for isolation of Escherichia coli O157:H7 from foods, Journal of Food Protection, 58 (1), 7-12.
• Worley, J.N., Flores, K.A, Yang, X., Chase, J.A, Cao, G., Tang, S., Meng, J., Atwill, E.R. (2017). Prevalence and genomic characterization of Escherichia coli O157:H7 in cow-calf herds throughout California. Appl. Environ. Microbiol, 83(16).
• Yücel, P.K., Halkman, H.B.D. (2009). Minimal İşlem Görmüş Meyve ve Sebzelerin Işınlama ile Kalitesinin Arttırılması. X. Ulusal Nükleer Bilimler ve Teknolojileri Kongresi, 6-9 Ekim, Muğla, 291-301.