EVALUATION OF EINKORN BRAN AS SUBSTRATE FOR SYNTHESIS OF ALPHA AMYLASE FROM PENICILLIUM HERQUEI IN SOLID STATE FERMENTATION PROCESS

Yıl 2018, Cilt: 8 Sayı: 2, 73 - 76, 30.11.2018

Seda Balkan

Öz

Solid State Fermentation (SSF) is a process defined as microorganisms
growing on a solid substrate in the absence of water. It is similar to natural
microbial processes such as composting and ensiling. This technique has been
known for a long time and is used widely in the Far East.  It can be also used in industrial
applications such as Roquefort cheese, antibiotics, organic acids, plant
growing agents, food additive materials and enzyme production. In this study,
α-amylase production was done in SSF process from Penicillium herquei. Agricultural by-products such as wheat (Triticum aestivum L.) bran and einkorn (Triticum monococcum L.) bran have been
tested for enzyme production, individually and as a mixture. The highest enzyme
activity (602 U/gds±5.6) was recorded in the SSF process where einkorn bran was
used as the substrate. The process parameters such as initial moisture level and
substrate amounts were optimized to determine their effects on enzyme synthesis
from P. herquei.

Anahtar Kelimeler

Solid State Fermentation, einkorn, Penicillium herquei, amylase

Kaynakça

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