Determination of Morphological Variation by Principal Component Analysis and Characterization of the Capsicum chinense Genetic Resources
Yıl 2021,
Cilt: 7 Sayı: 2, 86 - 105, 01.07.2021
Kübra Taş
,
Ahmet Balkaya
Öz
The characterization of plant genetic resources and genetic diversity levels are determined with the morphological descriptors
and molecular analysis methods. Capsicum chinense populations show a high level of variation in terms of fruit size, fruit
width, fruit shape, fruit colour and bitterness. This study aimed to define the plant characteristics of the C. chinense genetic
resources collected from different locations of the world according to the UPOV (International Union for the Protection
of New Varieties of Plants) criteria and to determine the morphological variation levels in the current population within
the species. In the first stage of this study, a gene pool consisting of 75 genotypes of the C. chinenses pecies was created.
It was found that genotypes belonging to the C. chinense species show a high level of phenotypic diversity in terms of
morphological identification criteria. Cluster and principal component analysis (PCA) were performed to determine
relationships among populations. A dendrogram clustered into seven groups was prepared to evaluate morphological
differences among C. chinense genotypes. In addition, the principal component (PC) analysis showed that the first six PC
axes explained 70.99% of the total multivariate variation. It revealed high morphological variation among the C. chinense
genotypes. In conclusion, this identified C. chinense genetic resources to be evaluated as qualified breeding materials for
developing new variety candidates in the near future.
Kaynakça
- Akıncı S and Akıncı İE, (2004). Evaluation of red
pepper for spice (Capsicum annuum L.)
germplasm resource of Kahramanmaraş Region
(Turkey). Pakistan Journal of Biological Sciences,
7 (5): 703-710.
- Alegbejo MD and Orakwue FC, (2002). Characteristics
of some pepper cultivars commonly grown in
Nigeria. Capsicum and Eggplant Newsletter, 21:
2-24.
- Balkaya A and Yanmaz R, (2001). Bitki genetik
kaynaklarının muhafaza imkanları ve tohum gen
bankalarının çalışma sistemleri. Ekoloji Çevre
Dergisi, 10(39): 25-30.
- Balkaya A, Yanmaz R, Apaydın A and Kar H
(2005). Morphological characterization of
white head cabbage (Brassica oleracea var.
capitata subvar. alba) genotypes in Turkey.
New Zealand Journal of Crop and Horticultural
Science, 33: 333-341.
- Balkaya A and Ergün A, (2008). Diversity and use of
pinto bean (Phaseolus vulgaris) populations from
Samsun, Turkey. New Zealand Journal of Crop
and Horticultural Science, 36: 189-197.
- Balkaya A, Özbakır M and Karaağaç O, (2010).
Karadeniz Bölgesinden toplanan bal kabağı
(Cucurbita moschata Duch.) populasyonlarının
karakterizasyonu ve meyve özelliklerindeki
varyasyonun değerlendirilmesi. Ankara Tarım
Bilimleri Dergisi, 16(1): 17-25.
- Balkaya A, (2013). Aşılı karpuz yetiştiriciliğinde
meyve kalitesini etkileyen faktörler. Journal of
TÜRKTOB, 2(6): 6-9.
- Barboza GE, Carrizo García C, Leiva González S,
Scaldaferro M, Reyes X, (2019) Four new species
of Capsicum (Solanaceae) from the tropical
Andes and an update on the phylogeny of the
genus. PLoS ONE 14(1): e0209792. https://doi.
org/10.1371/journal.pone.0209792
- Bayraktar K, (1970). Sebze Yetiştirme. Cilt II Kültür
Sebzeleri. Ege Üniversitesi Ziraat Fakültesi
Dergisi, 169- 479.
- Binbir S and Baş T, (2010). Bazı yerel biber (Capsicum
annuum L.) populasyonlarının karakterizasyonu.
Anadolu Ege Tarımsal Araştırma Enstitüsü
Dergisi, 20(2): 70-88.
- Bliss FA, (1981). Utilization of vegetable germplasm
(Ploidy levels). Hort Science, 16(2): 129-132.
- Brown JS, (1991). Principal component and cluster
analysis of cotton cultivar variability across the
U.S. Cotton Belt. Crop Science. 31: 915-922.
- Cherian EV and Indira P, (2003). Variability in
Capsicum chinense Jacq. germplasm. Capsicum
and Eggplant Newsletter, 22: 39-43.
- Conicella C, Errico A and Saccardo F, (1990).
Cytogenetic and isozyme studies of wild and
cultivated Capsicum annuum. Genome, 33: 279-
282.
- Deonton L and Vakinde MJ, (1993). Variation among
landraces of peppers in Nigeria. Capsicum and
Eggplant Newsletter, 12: 42-4.
- Duman İ and Düzyaman E, (2004). Türkiye’de
yetiştirilen bazı önemli biber genotiplerinin
morfolojik varyabilitesi üzerine bir araştırma.
Ege Üniversitesi Ziraat Fakültesi Dergisi, 41
(3): 55-56.
- Engels JMM, Arora RK and Guarino L, (1995). An
introduction to plant germplasm exploration and
collecting: planning, methods and procedures,
follow-up. Collecting plant genetic diversity.
Technical guidelines. CAB International,
Wallingford, United Kingdom, 31-63.
- Escribano MR, Santalla M, Casquero PA and Ron AM,
(1998). Patterns of genetic diversity in landraces
of common bean (Phaseolus vulgaris L.) from
Galicia. Plant Breeding, 117: 49-56.
- Eshbaugh WH, (2012). The taxonomy of the genus
Capsicum. In: Peppers Botany, Production and
Uses. CAB International, pp:14-28.
- Fonseca RM, Lopes R, Barros WS, Lopes MTG and
Ferreira FM, (2008) Morphologic characterization
and genetic diversity of Capsicum chinense Jacq.
accessions along the upper Rio Negro-Amazonas.
Crop Breeding and Applied Biotechnology, 8:187-
194. http://dx.doi.org/10.12702/1984-7033.
v08n03a02
- García-Neria MA and Rivera-Bustamante RF, (2011).
Characterization of geminivirus resistance in an
accession of Capsicum chinense Jacq. Molecular
Plant-Microbe Interactions, 24(2): 172-182.
- Geleta LF, Labuschagne MT and Viljoen CD, (2005).
Genetic variability in pepper (Capsicum annuum
L.) estimated by morphological data and amplified
fragment length polymorphism markers.
Biodiversity and Conservation, 14: 2361-2375.
- Gil J and Ron AM, (1992). Variation in Phaseolus
vulgaris in the Northwest of the Iberian
Peninsula. Plant Breeding, 109: 313-319.
Greenleaf WH, (1986). Pepper breeding. Breeding
Vegetable Crops. CAP International. The
Cambridge University Press, United Kingdom,
pp:76-82.
- Grubben GJH, (1977). Tropical vegetables and their
resources. IBPGR: International Board for Plant
Genetic Resources, 197 p., Rome, Italy.
Hallidri M and Tome E, (2000). Collection and
characterization of sweet pepper germplasm in
Albania. Capsicum and Eggplant Newsletter,
19: 46-49.
- Halstead HD, (1912). Experiments with peppers. N. J.
Agr. Exp. Sta. Ann. Rpt., 33: 365-368.
Hornakova O, Zavodna M, Zakova M, Kraic J and
Debre F (2003). Diversity of common bean
landraces collected in the western and eastern
Carpatien. Czech Journal of Genetics and Plant
Breeding, 39(3): 73-83.
- Hunter BR, (1993). Science based identification
of plant genetic material. CSSA, Intellectual
Property Rights: Protection of Plant Materials.
Special Publication No. 21:93-99.
- Ince AG, Karaca M and Onus AN, (2009). Development
and utilization of diagnostic DAMD-PCR
markers for Capsicum accessions. Genetic
Resources and Crop Evolution, 56: 211–221.
- Karaağaç O, (2006). Bafra kırmızı biber gen
kaynaklarının (Capsicum annuum var. conoides
Mill.) karakterizasyonu ve değerlendirilmesi.
Yüksek Lisans Tezi. Ondokuz Mayıs Üniversitesi
Fen Bilimleri Enstitüsü. Samsun. pp:129
- Karaağaç O and Balkaya A, (2010). Bafra kırmızı biber
populasyonlarının [Capsicum annuum L. var.
conoides (Mill.) Irish] tanımlanması ve mevcut
varyasyonun değerlendirilmesi. Anadolu Tarım
Bilimleri Dergisi, 25(1): 10-20.
- Karaağaç O and Balkaya A, (2017). Türkiye’de yerel
sebze çeşitlerinin mevcut durumu ve ıslah
programlarında değerlendirilmesi. TÜRKTOB,
23 (6): 8-15.
- Kurt O, (2001). Bitki ıslahı. OMÜ Ziraat Fakültesi.
Samsun. Ders Kitabı, 43: 309.
- Lefebvre V, Palloix A and Rives M, (1993). Nuclear
RFLP between pepper cultivars (Capsicum
annuum L.). Euphytica, 71: 189-199.
- Lefebvre V, Goffinet B, Chauvet JC, Caromel B,
Signoret P, Brand R and Palloix A, (2001).
Evaluation of genetic distances between pepper
inbred lines for cultivar protection purposes:
comparison of AFLP, RAPD and phenotypic
data. Theoretical and Applied Genetics, 102 (5):
741-750.
- Manju PR and Sreelathakumary I, (2004). Genetic
divergence in hot chili (Capsicum chinense Jaq.).
Capsicum and Eggplant Newsletter, 23: 69-72.
Mavi K, (2020). Biberlerde türler arası melezleme.
International Journal of Life Sciences and
Biotechnology, 3(3): 386-406.
- McLeod MJ, Guttman SI, Eshbaugh WH and Rayle RE,
(1983). An electrophoretic study of the evolution
in Capsicum (Solanaceae). Evolution, 37: 562-
574.
- Mohammadi SA and Prasanna BM, (2003). Analysis of
genetic diversity in crop plants-Salient statistical
tools and considerations. Crop Science, 43: 1235-
1248.
- Moscone EA, Scadalferro MA and Gabriele M, (2007).
The evolution of chili peppers (CapsicumSolanaceae) a cytogenetic perspective. Acta
Horticulturae, 745: 137–169.
- Ortiz R and Delgado DLF, (1990). Utilization of
descriptors for the characterization of lines of
the genus Capsicum. Turrialba, 40(1): 112-118.
- Ortiz R, de la Flor FD, Alvarado G and Crossa J,
(2010). Classifying vegetable genetic resources.
A case study with domesticated Capsicum spp.
Scientia Horticulturae, 126(2): 186-191.
- Otulaj AO and Makine MJ, (1994). Assesment of
the vegetative, reproductive characters and
fruit production pattern of pepper cultivars
(Capsicum spp.). Capsicum Eggplant
Newsletter, 13: 54-57.
- Pickersgill B, (1980). Some aspects of interspecific
hybridization in Capsicum. IVth Meeting of the
EUCARPIA Capsicum Working Group, pp:14-46
October 1980. Wageningen, Netherlands.
- Pickersgill B, (1984). Migrations of chili peppers,
Capsicum spp., in the Americas, D. Stone (ed.).
Pre-Columbian plant migration. Papers of the
Peabody Museum of Archeology and Ethnology.
vol. 76. Harvard University Press, pp:105-123,
Cambridge, Massachusetts.
- Ramchiary N, Kehie M, Brahma V, Kumaria S and
Tandon P, (2014). Application of genetics and
genomics towards Capsicum translational
research. Plant Biotechnol Reports, 8: 101-123.
- Rohlf FJ, (1993). Numerical Taxonomy and
Multivariate Analysis System. Exeter Software,
Dept. of Ecology and Evolution, State University
of New York.
- Smith JSC and Smith OS, (1989). The description and
assessment of distances between inbred lines of
maize: The utility of morphological, biochemical
and genetic descriptors and a scheme for the
testing of distinctiveness between inbredlines.
Maydica, 34: 151-161.
- Sneath PH and Sokal RR, (1973). Numerical Taxonomy.
The principles and practice of numerical
classification. 573 p.
- UPOV, (2006). Pepper (Capsicum annuum L.)
International Union For the Protection of New
Varieties of plants. TG/76/8, pp.51
Vasconcelos CSD, Barbieri RL, Neitzke RS, Priori
D, Fischer SZ and Mistura CC, (2012).
Determinação da dissimilaridade genética entre
acessos de Capsicum chinense com base em
características de flores. Revista Ceres, 59 (4):
493-498.
- Villota-Cerón D, Bonilla-Betancourt ML, CarmenCarrillo H, Jaramillo-Vásquez J and GarcíaDávila MA, (2012). Caracterización morfológica
de introducciones de Capsicum spp. existentes
en el Banco de Germoplasma activo de Corpoica
CI Palmira, Colombia. Acta Agronómica, 61(1):
16-26
- Zewdie Y and Zeven AC, (1997). Variation in
Yugoslavian hot pepper (Capsicum annuum L.)
accessions. Euphytica, 97: 81-89.
Yıl 2021,
Cilt: 7 Sayı: 2, 86 - 105, 01.07.2021
Kübra Taş
,
Ahmet Balkaya
Kaynakça
- Akıncı S and Akıncı İE, (2004). Evaluation of red
pepper for spice (Capsicum annuum L.)
germplasm resource of Kahramanmaraş Region
(Turkey). Pakistan Journal of Biological Sciences,
7 (5): 703-710.
- Alegbejo MD and Orakwue FC, (2002). Characteristics
of some pepper cultivars commonly grown in
Nigeria. Capsicum and Eggplant Newsletter, 21:
2-24.
- Balkaya A and Yanmaz R, (2001). Bitki genetik
kaynaklarının muhafaza imkanları ve tohum gen
bankalarının çalışma sistemleri. Ekoloji Çevre
Dergisi, 10(39): 25-30.
- Balkaya A, Yanmaz R, Apaydın A and Kar H
(2005). Morphological characterization of
white head cabbage (Brassica oleracea var.
capitata subvar. alba) genotypes in Turkey.
New Zealand Journal of Crop and Horticultural
Science, 33: 333-341.
- Balkaya A and Ergün A, (2008). Diversity and use of
pinto bean (Phaseolus vulgaris) populations from
Samsun, Turkey. New Zealand Journal of Crop
and Horticultural Science, 36: 189-197.
- Balkaya A, Özbakır M and Karaağaç O, (2010).
Karadeniz Bölgesinden toplanan bal kabağı
(Cucurbita moschata Duch.) populasyonlarının
karakterizasyonu ve meyve özelliklerindeki
varyasyonun değerlendirilmesi. Ankara Tarım
Bilimleri Dergisi, 16(1): 17-25.
- Balkaya A, (2013). Aşılı karpuz yetiştiriciliğinde
meyve kalitesini etkileyen faktörler. Journal of
TÜRKTOB, 2(6): 6-9.
- Barboza GE, Carrizo García C, Leiva González S,
Scaldaferro M, Reyes X, (2019) Four new species
of Capsicum (Solanaceae) from the tropical
Andes and an update on the phylogeny of the
genus. PLoS ONE 14(1): e0209792. https://doi.
org/10.1371/journal.pone.0209792
- Bayraktar K, (1970). Sebze Yetiştirme. Cilt II Kültür
Sebzeleri. Ege Üniversitesi Ziraat Fakültesi
Dergisi, 169- 479.
- Binbir S and Baş T, (2010). Bazı yerel biber (Capsicum
annuum L.) populasyonlarının karakterizasyonu.
Anadolu Ege Tarımsal Araştırma Enstitüsü
Dergisi, 20(2): 70-88.
- Bliss FA, (1981). Utilization of vegetable germplasm
(Ploidy levels). Hort Science, 16(2): 129-132.
- Brown JS, (1991). Principal component and cluster
analysis of cotton cultivar variability across the
U.S. Cotton Belt. Crop Science. 31: 915-922.
- Cherian EV and Indira P, (2003). Variability in
Capsicum chinense Jacq. germplasm. Capsicum
and Eggplant Newsletter, 22: 39-43.
- Conicella C, Errico A and Saccardo F, (1990).
Cytogenetic and isozyme studies of wild and
cultivated Capsicum annuum. Genome, 33: 279-
282.
- Deonton L and Vakinde MJ, (1993). Variation among
landraces of peppers in Nigeria. Capsicum and
Eggplant Newsletter, 12: 42-4.
- Duman İ and Düzyaman E, (2004). Türkiye’de
yetiştirilen bazı önemli biber genotiplerinin
morfolojik varyabilitesi üzerine bir araştırma.
Ege Üniversitesi Ziraat Fakültesi Dergisi, 41
(3): 55-56.
- Engels JMM, Arora RK and Guarino L, (1995). An
introduction to plant germplasm exploration and
collecting: planning, methods and procedures,
follow-up. Collecting plant genetic diversity.
Technical guidelines. CAB International,
Wallingford, United Kingdom, 31-63.
- Escribano MR, Santalla M, Casquero PA and Ron AM,
(1998). Patterns of genetic diversity in landraces
of common bean (Phaseolus vulgaris L.) from
Galicia. Plant Breeding, 117: 49-56.
- Eshbaugh WH, (2012). The taxonomy of the genus
Capsicum. In: Peppers Botany, Production and
Uses. CAB International, pp:14-28.
- Fonseca RM, Lopes R, Barros WS, Lopes MTG and
Ferreira FM, (2008) Morphologic characterization
and genetic diversity of Capsicum chinense Jacq.
accessions along the upper Rio Negro-Amazonas.
Crop Breeding and Applied Biotechnology, 8:187-
194. http://dx.doi.org/10.12702/1984-7033.
v08n03a02
- García-Neria MA and Rivera-Bustamante RF, (2011).
Characterization of geminivirus resistance in an
accession of Capsicum chinense Jacq. Molecular
Plant-Microbe Interactions, 24(2): 172-182.
- Geleta LF, Labuschagne MT and Viljoen CD, (2005).
Genetic variability in pepper (Capsicum annuum
L.) estimated by morphological data and amplified
fragment length polymorphism markers.
Biodiversity and Conservation, 14: 2361-2375.
- Gil J and Ron AM, (1992). Variation in Phaseolus
vulgaris in the Northwest of the Iberian
Peninsula. Plant Breeding, 109: 313-319.
Greenleaf WH, (1986). Pepper breeding. Breeding
Vegetable Crops. CAP International. The
Cambridge University Press, United Kingdom,
pp:76-82.
- Grubben GJH, (1977). Tropical vegetables and their
resources. IBPGR: International Board for Plant
Genetic Resources, 197 p., Rome, Italy.
Hallidri M and Tome E, (2000). Collection and
characterization of sweet pepper germplasm in
Albania. Capsicum and Eggplant Newsletter,
19: 46-49.
- Halstead HD, (1912). Experiments with peppers. N. J.
Agr. Exp. Sta. Ann. Rpt., 33: 365-368.
Hornakova O, Zavodna M, Zakova M, Kraic J and
Debre F (2003). Diversity of common bean
landraces collected in the western and eastern
Carpatien. Czech Journal of Genetics and Plant
Breeding, 39(3): 73-83.
- Hunter BR, (1993). Science based identification
of plant genetic material. CSSA, Intellectual
Property Rights: Protection of Plant Materials.
Special Publication No. 21:93-99.
- Ince AG, Karaca M and Onus AN, (2009). Development
and utilization of diagnostic DAMD-PCR
markers for Capsicum accessions. Genetic
Resources and Crop Evolution, 56: 211–221.
- Karaağaç O, (2006). Bafra kırmızı biber gen
kaynaklarının (Capsicum annuum var. conoides
Mill.) karakterizasyonu ve değerlendirilmesi.
Yüksek Lisans Tezi. Ondokuz Mayıs Üniversitesi
Fen Bilimleri Enstitüsü. Samsun. pp:129
- Karaağaç O and Balkaya A, (2010). Bafra kırmızı biber
populasyonlarının [Capsicum annuum L. var.
conoides (Mill.) Irish] tanımlanması ve mevcut
varyasyonun değerlendirilmesi. Anadolu Tarım
Bilimleri Dergisi, 25(1): 10-20.
- Karaağaç O and Balkaya A, (2017). Türkiye’de yerel
sebze çeşitlerinin mevcut durumu ve ıslah
programlarında değerlendirilmesi. TÜRKTOB,
23 (6): 8-15.
- Kurt O, (2001). Bitki ıslahı. OMÜ Ziraat Fakültesi.
Samsun. Ders Kitabı, 43: 309.
- Lefebvre V, Palloix A and Rives M, (1993). Nuclear
RFLP between pepper cultivars (Capsicum
annuum L.). Euphytica, 71: 189-199.
- Lefebvre V, Goffinet B, Chauvet JC, Caromel B,
Signoret P, Brand R and Palloix A, (2001).
Evaluation of genetic distances between pepper
inbred lines for cultivar protection purposes:
comparison of AFLP, RAPD and phenotypic
data. Theoretical and Applied Genetics, 102 (5):
741-750.
- Manju PR and Sreelathakumary I, (2004). Genetic
divergence in hot chili (Capsicum chinense Jaq.).
Capsicum and Eggplant Newsletter, 23: 69-72.
Mavi K, (2020). Biberlerde türler arası melezleme.
International Journal of Life Sciences and
Biotechnology, 3(3): 386-406.
- McLeod MJ, Guttman SI, Eshbaugh WH and Rayle RE,
(1983). An electrophoretic study of the evolution
in Capsicum (Solanaceae). Evolution, 37: 562-
574.
- Mohammadi SA and Prasanna BM, (2003). Analysis of
genetic diversity in crop plants-Salient statistical
tools and considerations. Crop Science, 43: 1235-
1248.
- Moscone EA, Scadalferro MA and Gabriele M, (2007).
The evolution of chili peppers (CapsicumSolanaceae) a cytogenetic perspective. Acta
Horticulturae, 745: 137–169.
- Ortiz R and Delgado DLF, (1990). Utilization of
descriptors for the characterization of lines of
the genus Capsicum. Turrialba, 40(1): 112-118.
- Ortiz R, de la Flor FD, Alvarado G and Crossa J,
(2010). Classifying vegetable genetic resources.
A case study with domesticated Capsicum spp.
Scientia Horticulturae, 126(2): 186-191.
- Otulaj AO and Makine MJ, (1994). Assesment of
the vegetative, reproductive characters and
fruit production pattern of pepper cultivars
(Capsicum spp.). Capsicum Eggplant
Newsletter, 13: 54-57.
- Pickersgill B, (1980). Some aspects of interspecific
hybridization in Capsicum. IVth Meeting of the
EUCARPIA Capsicum Working Group, pp:14-46
October 1980. Wageningen, Netherlands.
- Pickersgill B, (1984). Migrations of chili peppers,
Capsicum spp., in the Americas, D. Stone (ed.).
Pre-Columbian plant migration. Papers of the
Peabody Museum of Archeology and Ethnology.
vol. 76. Harvard University Press, pp:105-123,
Cambridge, Massachusetts.
- Ramchiary N, Kehie M, Brahma V, Kumaria S and
Tandon P, (2014). Application of genetics and
genomics towards Capsicum translational
research. Plant Biotechnol Reports, 8: 101-123.
- Rohlf FJ, (1993). Numerical Taxonomy and
Multivariate Analysis System. Exeter Software,
Dept. of Ecology and Evolution, State University
of New York.
- Smith JSC and Smith OS, (1989). The description and
assessment of distances between inbred lines of
maize: The utility of morphological, biochemical
and genetic descriptors and a scheme for the
testing of distinctiveness between inbredlines.
Maydica, 34: 151-161.
- Sneath PH and Sokal RR, (1973). Numerical Taxonomy.
The principles and practice of numerical
classification. 573 p.
- UPOV, (2006). Pepper (Capsicum annuum L.)
International Union For the Protection of New
Varieties of plants. TG/76/8, pp.51
Vasconcelos CSD, Barbieri RL, Neitzke RS, Priori
D, Fischer SZ and Mistura CC, (2012).
Determinação da dissimilaridade genética entre
acessos de Capsicum chinense com base em
características de flores. Revista Ceres, 59 (4):
493-498.
- Villota-Cerón D, Bonilla-Betancourt ML, CarmenCarrillo H, Jaramillo-Vásquez J and GarcíaDávila MA, (2012). Caracterización morfológica
de introducciones de Capsicum spp. existentes
en el Banco de Germoplasma activo de Corpoica
CI Palmira, Colombia. Acta Agronómica, 61(1):
16-26
- Zewdie Y and Zeven AC, (1997). Variation in
Yugoslavian hot pepper (Capsicum annuum L.)
accessions. Euphytica, 97: 81-89.