In vitro Antibacterial Activity of Naproxen and its Combination with Ciprofloxacin

Yıl 2024, Cilt: 7 Sayı: 1, 25 - 30, 22.07.2024

Sultan Öğmen Seven , Ertuğrul Özbil , Açelya Mavideniz , Mehmet İlktaç

https://doi.org/10.54994/emujpharmsci.1506693

Öz

Naproxen, a nonsteroidal anti-inflammatory drug (NSAID), is commonly used to reduce fever, and to treat pain and inflammation caused by several conditions. Previously, naproxen was evaluated for its antimicrobial potency in various studies. In our study, we aimed to demonstrate the antibacterial and synergistic activities of naproxen and ciprofloxacin against various Gram-positive and Gram-negative bacteria including, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 700603. The results showed promising antibacterial activity against tested Gram-positive bacteria. However, there was no effect on Gram-negative bacteria. Additionally, checkerboard assays did not reveal any additive or synergistic activity when combined with Ciprofloxacin. Collectively, our study's data show naproxen's selectivity against Gram-positive bacteria. This result suggests that naproxen can be further used as a potential source of antibiotics against Gram-positive bacteria.

Anahtar Kelimeler

Antibacterial, Checkerboard, Ciprofloxacin, Enterecoccus faecalis, Naproxen, Staphylococcus aureus.

Kaynakça

• Bellio P, Fagnani L, Nazzicone L and Celenza G (2021). New and simplified method for drug combination studies by checkerboard assay. MethodsX 8: 101543.
• Han IM and Kucukguzel G (2020). Anticancer and antimicrobial activities of Naproxen and Naproxen derivatives. Mini-Rev Med Chem 20: 1300-1310.
• Hasan MS and Das N (2019). A detailed in vitro study of naproxen metal complexes in quest of new therapeutic possibilities. Alexandria Med J 53: 157-165.
• Hussein AA and Al-Janabi S (2011). Investigation of antidermatophytic effects of non-steroidal anti-inflammatory drugs on trichophyton mentagrophytes and epidermophyton floccosum. Iran J Pharm Res 10: 547-552.
• Mamatha N, Babu NS, Mukkanti K and Pal S (2011). 2-(6-methoxynaphthalen-2-yl)propionic acid (1,3-dimethyl-butylidene)-hydrazide. Molbank 4: 1-4.
• Stoev SS, Gueorguiev SS, Madzharov VG and Lebanova HV (2021). Naproxen in pain and inflammation – a review. Int J Pharm Clin 11: 142-148.
• Wikler MA (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard, vol. 26, p M7–A7. CLSI, Wayne, Pennsylvania.