Mortality effects of eicosanoid biosynthesis inhibitors on Spodoptera littoralis larvae co-injected with the bacteria, Serratia marcescens

Yıl 2015, Cilt: 39 Sayı: 2, 121 - 127, 30.01.2015

Hasan Tunaz , Mustafa Küsek

https://doi.org/10.16970/ted.10222

Cited By: 1

Öz

The first step of the cellular defense reactions to bacterial, fungal and some viral infections in insects is nodulation. We posed the hypothesis that Spodoptera littoralis, expresses melanoic nodulation reactions to bacterial challenge and that injecting S. littoralis larvae with eicosanoid biosnthesis inhibitors (EBIs) plus bacteria would increase larval mortality. Injecting larvae with EBIs, immediately before intrahemocoelic injections of the bacterium, Serratia marcescens, sharply reduced the nodulation response to bacterial challenges. Separate treatments with specific inhibitors including dexamethasone (a phospholipase A₂ inhibitor), indomethacin, naproxen, ibuprofen, (cyclooxygenase inhibitors), esculetin (a lipoxygenase inhibitor) and Phenidone (dual cyclooxgenase/lipoxygenase inhibitor) impaired the ability of S. littoralis to form nodules in reaction to bacterial challenge. All concentrations of S. marcescens alone, applied to S. littoralis, caused low mortality of the larvae. However, an increased mortality of the larvae was seen when S. marcescens was co-injected with the EBIs with different modes of action. These findings support our hypothesis that virulent effects of entomopathogenic bacteria can be increased when S. littoralis immune systems were suppressed.  

Anahtar Kelimeler

Insect cellular immunity, nodulation, eicosanoid, bacteria, Spodoptera littoralis

Eikosanoid biyosentezi inhibitörleri Serratia marcescens bakterisi ile birlikte Spodoptera littoralis larvalarına uygulandığında larvalar üzerindeki ölüm etksi

Öz

Böceklerde bakeri, fungus ve bazı virüs hastalıklarına karşı oluşan hücresel bağışıklıklardan ilki nodülasyon reaksiyonudur. Bu çalışmada Spodoptera littoralis larvalarında Serratia marcescens bakterisine karşı oluşan nodülasyon reaksiyonu, bakterinin larvalar üzerindeki ölüm etkisi ve bakteri ile birlikte eiokosanoid biyosentezi inhibitörleri larvalara uygulandığında larvaların ölüm oranını etkileyip etkilemeyeceği test edilmiştir. S. littoralis larvalarına bakteri uygulamasında hemen önce eiokosanoid biyosentezi inhibitörleri enjekte edildiğinde böceklerede bakteriye karşı oluşan nodül sayısında önemli derecede azalma olmuştur. Tüm S. Marcescens bakteri konsantrasyonları larvalara tek başına uygulandığında düşük oranda larva ölümü ortaya çıkmıştır. Diğer taraftan S. marcescens bakterisi ile birlikte eikosanoid biyosentezi inhibitörleri S. littoralis larvalarına uygulandığında larvalar üzerindeki ölüm oranı yalnız bakteri uygulanan larvalara oranla önemli derecede yükselmiştir. Bu bulgular böceklerin bağışıklık sistemi baskı altına alındığında,  S. marcescens bakterisinin entomopatojen etkisinin arttığını göstermiştir. 

Anahtar Kelimeler

Böcek hücresel bağışıklığı, nodülasyon, eikosanoid, bakteri, Spodoptera littoralis

Kaynakça

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