The effects of final irrigants on the push-out bond strength of two calcium silicate-based root canal sealers: an in vitro study

Yıl 2021, Cilt: 55 Sayı: 3, 146 - 151, 21.09.2021

Davut Çelik , Ayse Tuba Özalp Koca , Tuğba Koşar , Tamer Taşdemir

https://doi.org/10.26650/eor.2021922696

Öz

Purpose The purpose of the present study was to investigate the effect of the different irrigant combinations used in final irrigation on the push-out bond strength of root canal sealers that have different compositions. Materials and Methods In total 60 dentinal slices in 1 mm thickness were collected from 15 extracted mandibular premolar teeth; 4 slices from each tooth. 3 canal-like artificial cavities were opened on each dentinal slice. Samples were divided into 4 experimental groups, each of which consisted of 15 samples. In group 1, samples were immersed in 5.25% NaOCl and 17% EDTA solutions respectively; in group 2, immersed in 5.25% NaOCl and 2% CHX solutions respectively; in group 3, immersed in 5.25% NaOCl, 17% EDTA and 2% CHX solutions respectively; and in group 4 immersed in distilled water. After drying with absorbent papers, each cavity in dentinal slice sample was filled with different sealer (Endoseal MTA, Tech Biosealer Endo or AH Plus). Two days later, the push-out bond examination was performed. Results AH Plus showed higher push-out bond strength value in two combinations (group 2 and 3) in which final irrigants contained CHX (p<0.001). Dentinal push-out bond strengths of root canal sealers from Endoseal MTA and Tech BioSealer Endo were not affected by final irrigant (p=0.965). Conclusion Using CHX after NaOCl in final irrigant increases push-out strength of epoxy resin- based sealer but, did not create any difference in dentinal push-out bond strength of calcium silicate-based sealers. 

Anahtar Kelimeler

Calcium silicate-based sealer, chlorhexidine gluconate, precipitate, push- out bond strength, root canal irrigants

Kaynakça

• 1. Dugas NN, Lawrence HP, Teplitsky PE, Pharoah MJ, Friedman S. Periapical health and treatment quality assessment of root- filled teeth in two Canadian populations. Int Endod J 2003; 36: 181–92. [CrossRef ]
• 2. Ureyen Kaya B, Kececi AD, Guldas HE, Orhan H. A retrospective radiographic study of coronal-periapical status and root canal filling quality in a selected adult Turkish population. Med Princ Pract 2013; 22: 334–9. [CrossRef ]
• 3. ElAyouti A, Achleithner C, Löst C, Weiger R. Homogeneity and adaptation of a new gutta-percha paste to root canal walls. J Endod 2005; 31: 687–90. [CrossRef ]
• 4. Saleh I, Ruyter I, Haapasalo M, Ørstavik D. The effects of dentine pretreatment on the adhesion of root-canal sealers. Int Endod J 2002; 35: 859–66. [CrossRef ]
• 5. Schwartz RS. Adhesive dentistry and endodontics. Part 2: bonding in the root canal system-the promise and the problems: a review. J Endod 2006; 32: 1125–34. [CrossRef ]
• 6. Thomas JE, Sem DS. An in vitro spectroscopic analysis to determine whether para-chloroaniline is produced from mixing sodium hypochlorite and chlorhexidine. J Endod 2010; 36: 315–7. [CrossRef ]
• 7. Zehnder M, Schmidlin P, Sener B, Waltimo T. Chelation in root canal therapy reconsidered. J Endod. 2005; 31: 817-20. [CrossRef ]
• 8. Haapasalo M, Shen Y, Wang Z, Gao Y. Irrigation in endodontics. Br Dent J 2014; 216: 299-303. [CrossRef ]
• 9. Clarkson RM, Podlich HM, Moule AJ. Influence of ethylenediaminetetraacetic acid on the active chlorine content of sodium hypochlorite solutions when mixed in various proportions. J Endod 2011; 37: 538–43. [CrossRef ]
• 10. Hülsmann M, Rödig T, Nordmeyer S. Complications during root canal irrigation. Endod Topics 2009; 16: 27–63. [CrossRef ]
• 11. Hashem AA, Ghoneim AG, Lutfy RA, Fouda MY. The effect of different irrigating solutions on bond strength of two root canal-filling systems. J Endod 2009; 35: 537–40. [CrossRef ]
• 12. Naenni N, Thoma K, Zehnder M. Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod 2004; 30: 785–7. [CrossRef ]
• 13. Zehnder M. Root canal irrigants. J Endod 2006; 32: 389–98. [CrossRef ]
• 14. Magro MG, Kuga MC, Regina Victorino K, Vázquez-Garcia FA, Aranda-Garcia AJ, Faria-Junior NB, Faria G, Shinohara AL. Evaluation of the interaction between sodium hypochlorite and several formulations containing chlorhexidine and its effect on the radicular dentin - SEM and push-out bond strength analysis. Microsc Res Tech 2014; 77: 17-22. [CrossRef ]
• 15. Kuruvilla JR, Kamath MP. Antimicrobial activity of 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate separately and combined, as endodontic irrigants. J Endod 1998; 24: 472–6. [CrossRef ]
• 16. Bui T, Baumgartner J, Mitchell J. Evaluation of the interaction between sodium hypochlorite and chlorhexidine gluconate and its effect on root dentin. J Endod 2008; 34: 181–5. [CrossRef ]
• 17. Burkhardt-Holm P, Oulmi Y, Schroeder A, Storch V, Braunbeck T. Toxicity of 4-chloraniline in early life stages of Zebrafish (Danio rerio): II—cytopathology and regeneration of liver and gills after prolonged exposure to waterborne 4 chloraniline. Arch Environ Contam Toxicol 1999; 37: 85–102. [CrossRef ]
• 18. Basrani BR, Manek S, Sodhi RNS, Fillery E, Manzur A. Interaction between sodium hypochlorite and chlorhexidine gluconate. J Endod 2007; 33: 966–9. [CrossRef ]
• 19. Orhan EO, Irmak Ö, Hür D, Yaman BC, Karabucak B. Does Para- chloroaniline really form after mixing sodium hypochlorite and chlorhexidine? J Endod 2016; 42: 455–9.
• 20. Irmak Ö, Orhan EO, Görgün K, Yaman BC. Nuclear magnetic resonance spectroscopy and infrared spectroscopy analysis of precipitate formed after mixing sodium hypochlorite and QMix 2in1. PLoS One 2018; 13: e0202081.
• 21. Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of irrigation sequence on the adhesion of root canal sealers to dentin: a fourier transform infrared spectroscopy and push-out bond strength analysis. J Endod 2015; 41: 1108–11. [CrossRef ]
• 22. Scelza MZ, da Silva D, Scelza P, de Noronha F, Barbosa IB, Souza E, De Deus G. Influence of a new push-out test method on the bond strength of three resin-based sealers. Int Endod J 2015; 48: 801–6. [CrossRef ]
• 23. Bitter K, Meyer-Lueckel H, Priehn K, Kanjuparambil JP, Neumann K, Kielbassa AM. Effects of luting agent and thermocycling on bond strengths to root canal dentine. Int Endod J 2006; 39: 809- 18. [CrossRef ]
• 24. Silva EJ, Carvalho NK, Zanon M, Senna PM, DE-Deus G, Zuolo ML, Zaia AA. Push-out bond strength of MTA HP, a new high- plasticity calcium silicate-based cement. Braz Oral Res 2016; 30: e84.
• 25. Silva EJ, Carvalho NK, Prado MC, Zanon M, Senna PM, Souza EM, De-Deus G. Push-out bond strength of injectable Pozzolan- based root canal sealer. J Endod 2016; 42: 1656–9. [CrossRef ]
• 26. Silva EJNL, Carvalho NK, Prado MC, Senna PM, Souza EM, De- Deus G. Bovine teeth can reliably substitute human dentine in an intra-tooth push-out bond strength model? Int Endod J 2019; 52: 1063–9.
• 27. Magro MG, Kuga MC, Aranda-Garcia AJ, Victorino KR, Chávez- Andrade GM, Faria G, Keine KC, Só MVR. Effectiveness of several solutions to prevent the formation of precipitate due to the interaction between sodium hypochlorite and chlorhexidine and its effect on bond strength of an epoxy-based sealer. Int Endod J 2015; 48: 478–83. [CrossRef ]
• 28. Prati C, Chersoni S, Pashley DH. Effect of removal of surface collagen fibrils on resindentin bonding. Dent Mater 1999; 15: 323–31. [CrossRef ]
• 29. Fisher MA, Berzins DW, Bahcall JK. An in vitro comparison of bond strength of various obturation materials to root canal dentin using a push-out test design. J Endod 2007; 33: 856–8. [CrossRef ]
• 30. Güzel C, Uzunoglu E, Dogan Buzoglu H. Effect of low–surface tension EDTA solutions on the bond strength of resin-based sealer to young and old root canal dentin. J Endod 2018; 44: 485–8. [CrossRef ]
• 31. Oliveira DS, Cardoso ML, Queiroz TF, Silva EJNL, Souza EM, De-Deus G. Suboptimal push-out bond strengths of calcium silicate-based sealers. Int Endod J 2016; 49: 796–801. [CrossRef ]
• 32. Silva EJNL, Canabarro A, Andrade MRTC, Cavalcante DM, Von Stetten O, Fidalgo TKDS, De-Deus G. Dislodgment resistance of bioceramic and epoxy sealers: a systematic review and meta- analysis. J Evid Base Dent Pract 2019; 19: 221-35. [CrossRef ]
• 33. Neelakantan P, Nandagopal M, Shemesh H, Wesselink P. The effect of root dentin conditioning protocols on the push-out bond strength of three calcium silicate sealers. Int J Adhes Adhes 2015; 60: 104–8. [CrossRef ]