Kültüre Edilen Sivriburun Karagöz Balıklarında (Diplodus puntazzo Cetti, 1777) Micrococcus luteus Enfeksiyonunun Teşhisi

Yıl 2020, Cilt: 36 Sayı: 2, 214 - 220, 17.08.2020

Tülay Akaylı , Remziye Eda Yardımcı , Çiğdem Ürkü

Öz

Bu
çalışmada Ege Denizi'nde kültüre edilen sivriburun karagöz balıklarında (Diplodus puntazzo Cetti, 1777) meydana
gelen ölümlerin nedeninin belirlenmesi amaçlandı. Mayıs 2009 ve Mayıs 2010
tarihleri ​​arasında bakteriyolojik yöntemler kullanılarak toplam 10 hasta
birey (200-350g) incelendi. Hasta balık örneklerinde solgun solungaç ve
karaciğer, deri renginde açılma, karaciğerde ve kaslarda kanamalar görüldü.
Hasta balıkların karaciğer, böbrek ve dalağından Marine Agar (MA) ve % 1,5 NaCl
içeren Tryptic Soy Agar (TSA) yapılan bakteriyolojik ekimler 22 ° C'de 48 saat
süreyle inkübe edildi. Gram pozitif hareketli olmayan koklar viseral
organlardan izole edildi. Morfolojik ve biyokimyasal özelliklerine göre, bu
izolatlar Miccrococcus luteus olarak identifiye edildi. Kirby-Bauer
disk difüzyon yöntemi çoklu disk kullanılarak yapıldı. Sonuçlar mevcut
verilerine dayanılarak yorumlandı ve tüm izolatların oksitetrasiklin,
eritromisin ve kloramfenikole karşı duyarlı olduğu belirlendi. Histopatolojik
olarak karaciğer parankim hücrelerinde vakuolar dejenerasyon ve nekroz,
böbreklerde tübüler nekroz ve dalakta hemosiderin depozitleri saptandı. Lamellalarda
erimenin yanı sıra solungaçlarda epitel hücre hiperplazisi gözlendi. Sonuç
olarak, bu çalışmada M. luteus’un neden olduğu bir düşük mortaliteli bir epizootik tanımlandı
ve bu çalışma bu patojenin kültür sivriburun karagöz balıklarında tanımlandığı ilk
rapordur.

Anahtar Kelimeler

Sivriburun Karagöz, Kafes Yetiştiriciliği, Micrococcus luteus

Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)

Öz

The aim of the study was to determinate the cause of
mortalities in cultured sharpsnout sea bream (Diplodus puntazzo Cetti, 1777) in the Aegean Sea. Ten individuals
(200-350 g) in total between May 2009 – 2010 were investigated using
bacteriological methods. Moribund fish samples exhibited pale gills and liver,
skin depigmentation, haemorrhaging in the liver and muscles. Bacteriological
samples of liver, kidney and spleen were streaked onto Marine Agar (MA) and
Tryptic Soy Agar (TSA) plates containing %1,5 NaCl and incubated at 22°C for
48h. Gram-positive non-motile cocci were isolated from visceral organs.
According to their morphological and biochemical characteristics, these
isolates were identified as Micrococcus
luteus. The Kirby-Bauer disk
diffusion method was also performed using multidisc. Results were interpreted
based on the available data and all isolates were determined to be sensitive
against oxytetracycline, erythromycin and chloramphenicol. Histopathologically,
vacuolar degeneration and necrosis in the parenchyma cells of liver, tubular
necrosis in the kidney and hemosiderin deposits in the spleen were determined.
In addition to melting lamella, epithelial cells hyperplasia
in the gills was observed. In conclusion, in this study an epizootic with low
mortality caused M. luteus were
described and this study is the first report of the pathogen in the cultured
sharpsnout sea bream.

Anahtar Kelimeler

Sharpsnout Sea Bream, Cage Culture, Micrococcus luteus

Kaynakça

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