Ralstonia eutropha H16 suşuyla sulu çözeltilerden kurşun ve bor biyogiderimi

Yıl 2021, Cilt: 37 Sayı: 2, 314 - 322, 28.08.2021

Kâmil Varınca , Miraç Uçkun , Şeyma Akkurt , Aysel Alkan Uçkun

Öz

Öz: Bu çalışmada, su ortamlarına en fazla karışan ve canlılar üzerinde önemli toksik etkileri bulunan kurşun (Pb) ile kullanımı giderek yaygınlaşan ve ülkemiz için oldukça büyük stratejik öneme sahip olan bor (B) elementinin sulardan gideriminde, geleneksel yöntemlere alternatif olarak daha ekonomik, kullanışlı ve etkin bir yöntem olan biyolojik arıtımın kullanılması amaçlanmıştır. Bu amaçla, Ralstonia eutropha H16 suşunun Pb ve B bağlama kapasitesi test edilmiştir. Minimum İnhibisyon Konsantrasyonları (MİK), Pb için 1365 ppm, B için 2500 ppm olarak belirlenmiştir. Pb ve B’un üç alt dozu (Pb için 5, 25, 50 ppm; B için 500, 1000, 1500 ppm) bakterilere 24 saat boyunca uygulanmış ve bu sürenin sonunda kültür ortamında, bakteriyel hücrelerin yüzeyinde ve içinde olmak üzere üç farklı ortamda biriken Pb ve B konsantrasyonları bir İndüktif Eşleşmiş Plazma Kütle Spektrometresi (ICP-MS) kullanılarak tayin edilmiştir. En fazla Pb biyogiderimi %88’lik bir oranla 5 ppm Pb uygulamasında, en fazla B giderimi ise %7’lik bir oranla 500 ppm B uygulamasında gözlenmiştir. Taramalı Elektron Mikroskobu (SEM) görüntüleri, Pb uygulanan hücrelerin yüzeyinin kontrole göre pürüzlü olduğunu ve önemli derecede çökelmeler olduğunu göstermiştir. Enerji Dağılımlı X-ışını Spektroskopisi (EDX) spektrumları ise Pb uygulanan hücrelerde Pb varlığını doğrulamıştır. Çalışmanın sonuçları, R. eutropha H16 suşunun sulardan Pb gideriminde önemli derecede etkili olduğunu ancak B gideriminde yeterli olmadığını göstermiştir.

Anahtar Kelimeler

Biyosorpsiyon, Ralstonia eutropha, Atıksuların biyolojik arıtımı, Sulardan metal biyogiderimi, Bakterilerin SEM görüntüleri

Kaynakça

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