Voltammetric Determination of Rutin by Using Disposable Pencil Graphite Electrode

Yıl 2019, Cilt: 12 Sayı: 1, 158 - 169, 24.03.2019

Kuddusi Karaboduk

https://doi.org/10.18185/erzifbed.432836

Cited By: 3

Öz

In this study, an
electrochemical method was developed for the determination of rutin. The pencil
graphite electrode, a disposable and low cost electrode, showed a very good
catalytic effect with the significant enhancement of the peak current compared
to the glassy carbon electrode. Under optimized conditions, the pencil graphite
electrode had two linear ranges from 0.39 to 166.70×10^-7 M and
166.70 to 1060.60×10^-7 M rutin, the limit of detection was also obtained
1.13 nM (S/N=3). The percentage of recoveries were obtained in a range between
98.98 and 101.02 % for five successive determinations of rutin, which indicate
acceptable repeatability. This method was successfully applied for the direct
determination of rutin in real samples such as buckwheat, green tea and red apple. Finally, the interference
effects of some species to the determination of rutin was also evaluated.

Anahtar Kelimeler

Flavonoid, Rutin, Pencil graphite electrode, Voltammetric determination

Tek Kullanımlık Kalem Grafit Elektrot Kullanılarak Rutinin Voltametrik Tayini

Öz

Bu çalışmada, rutinin tayini
için elektrokimyasal bir yöntem geliştirildi. Kalem grafit elektrot tek
kullanımlık ve düşük maliyetli bir elektrottur. Kalem grafit elektrot, camsı
karbon elektrota kıyasla pik akımının önemli ölçüde artmasıyla çok iyi bir
katalitik etki göstermiştir. Optimize edilmiş koşullar altında, kalem grafit
elektrot, 0.104 ile 166.70×10^-7 M ve 166.70
ile 1060.60×10^-7 M rutin olmak üzere iki doğrusal yanıta sahiptir, Tespit ve tayin limitleri sırasıyla 1.13×10^-9
M (S / N = 3) ve 3.48×10^-9 M olarak hesaplandı. Yüzde geri kazanım, kabul
edilebilir tekrarlanabilirliği gösteren, ardışık beş tekrarlı rutin analizi
için % 98.98 ile % 101.02 arasında bir aralıkta elde edilmiştir. Geliştirilen yöntem;
karabuğday, yeşil çay ve kırmızı elma gibi gerçek numunelerde, rutinin doğrudan
tayini için başarıyla uygulanmıştır. Son olarak, bazı türlerin rutin tayinine
girişim etkileri de değerlendirilmiştir.

Anahtar Kelimeler

Flavonoid, Rutin, Kalem grafit elektrot, Voltametrik tayin

Kaynakça

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