EFFECT OF SOME DRUGS ON SHEEP LIVER CARBONIC ANHYDRASE I AND II

Yıl 2015, Cilt: 8 Sayı: 2, 201 - 212, 16.12.2015

Murat Çankaya , Mehmet Kuzucu

https://doi.org/10.18185/eufbed.22429

Cited By: 1

Öz

Effects of clindamycin, dexketoprofen trometamol, Benzylpenicillin, (penicillin G), Phenoxymethylpenicillin (penicillin V) were examined on purified sheep carbonic anhydrase I and II (SCAI, SCA-II). In our study, we were chosen sheep liver tissue; because the liver is responsible for detoxification of xenobiotics. The CA isoenzyme have a very important duties on organisms, the effect of xenobiotics on the CA enzyme was reported. We exhibited the inhibition effects of these drugs against two sheep liver carbonic anhydrase isozymes (SCA I and SCA II). SCA I and SCA II isozymes were purified with about a specific activity  of 1108,51 EU/mg and 5706,67 EU/mg,  120,62 and  620,96 purification fold by using Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Purity of the SCA I and SCA II isoenzyme was checked by SDS-PAGE technique and subunit molecular masses of the SCA I was found as 29 kDa and SCA II found as 31 kDa. The usage of clindamycin, dexketoprofen trometamol, Benzylpenicillin, (penicillin G), Phenoxymethylpenicillin (penicillin V) in sheep liver especially during necrotic hepatitis disease is important for scientific researches. As most of the antibiotic and antihistaminic can be dangerous for sheep liver. Also, it is important to study the inhibition effects of these drugs on SCA I and II izoenzymes.

Anahtar Kelimeler

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BAZI İLAÇLARIN KOYUN KARACİĞERİ KARBONİK ANHİDRAZ I VE II ÜZERİNDEKİ ETKİSİ

Öz

Koyun karaciğerinden saflaştırılmış karbonik anhidraz I ve II (SCAI, SCA-II) üzerine; klindamisin, deksketoprofen trometamol, benzilpenisilin (penisilin G) ve fenoksimetilpenisilin (penisilin V)’in etkileri incelenmiştir. Çalışmamızda koyun karaciğer dokusunu seçtik, çünkü karaciğer ksenobiyotiklerin detoksifikasyonundan sorumludur. Karbonik anhidraz izoenzimlerinin ksenobiyotiklerin karbonik anhidraz üzerindeki etkileri rapor edilmiştir. organizmalarda çok önemli görevleri vardır, Çalışmamızda bu ilaçların, koyun karaciğeri karbonik anhidraz I ve II izoenzimlerine karşı inhibisyon etkisi gösterdiği gözlenmiştir. SCA I ve SCA II kromatografisi kullanılarak, yaklaşık olarak 1108.51 EU/mg ve 5706.67 EU/mg spesifik aktivite ile 120.62 ve 620.96 kat saflaştırıldı. SCA I ve SCA II izoenzimlerinin saflığı SDS-PAGE tekniği ile tespit edildi ve alt birimlerinin ağırlığı SCA I’de 29 kDa ve SCA II’de 31 kDa olarak belirlendi. Koyun karaciğerinde, özellikle nekrotik hepatitis hastalığı sırasında kullanılan; klindamisin, deksketoprofen trometamol, benzilpenisilin (penisilin G) ve fenoksimetilpenisilin (penisilin V) hakkında önemli araştırmalar yapılmaktadır. Birçok antibiyotik ve antihistamin koyun karaciğeri için tehlikeli etkilere sahip olabilir. Ayrıca, bu ilaçların SCA I ve II izoenzimleri üzerinde inhibisyon etkilerini incelemek oldukça önemlidir

Anahtar Kelimeler

Karaciğer, Antibiyotik, İnhibisyon

Kaynakça

• Ayvaz, S., Cankaya, M., Atasever, A. Altuntas, A. 2013. 2-Amino-3- cyanopyridine derivatives as carbonic anhydrase inhibitors, Journal of Enzyme Inhibition and Medicinal Chemistry, 28, 305- 310.
• Barbanoj, M.J., Antonijoan, R.M., Gich, I. 2001. Clinical pharmacokinetics of Dexketoprofen, Clinical Pharmacokinetics, 40, 245–262.
• Beydemir, S., Ciftçi, M., Ozmen, I., Buyukokuroglu, M.E., Ozdemir, H., Küfrevioglu, O.I. 2000. Effects of some medical drugs on enzyme activities of carbonic anhydrase from human erythrocytes in vitro and from rat erythrocytes in vivo, Pharmacological Research, 42, 187-191.
• Bradford, M. 1979. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry, 72, 248-254.
• Brinkman, R., Margaria, R., Meldrum, N.U., Roughton, F.J.W. 1932. The CO2 catalyst present in blood, The Journal of Physiology, 75, 3-4.
• Britta, K., Ralf, R. 1997. β-lactam antibiotics inhibit chloroplast division in a moss (Physcomitrella patens) but not in tomato (Lycopersicon esculentum), Journal of Plant Physiology, 150, 137- 140.
• Brook, I., Lewis, M.A.O., Sandor, G.K.B., Jeffcoat, M., Samaranayake, L.P., Rojas, J.V. 2005. Clindamycin in dentistry: More than just effective prophylaxis for endocarditis?, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 100, 550-558.
• Cankaya, M., Aktas, M., Kuzucu, M., Gül, I., Coban, T.A. 2012. Effects of some drugs on human cord blood erythrocyte carbonic anhydrases I and II: an in vitro study, Journal of Enzyme Inhibition and Medicinal Chemistry, 27, 641-645.
• Chegwidden, W.R., Carter, N.D. 2000. Introduction to the carbonic anhydrases, EXS, 90, 13-28.
• Chiche, J.I., Laferrière, K., Trottier, J., Dayan, E., Mazure, F., Brahimi, Horn, N.M., Pouysségur, M.C. 2009. Hypoxia-inducible carbonic anhydrase IX and XII promote tumor cell growth by counteracting acidosis through the regulation of the intracellular pH, Cancer Research, 69, 358–368.
• Ciftci, M., Kufrevioglu, O.I., Gundogdu, M., Ozmen, I. 2000. Effects of some antibiotics on enzyme activity of glucose 6-phosphate dehydrogenase from the human erythrocytes, Pharmacol Research, 41, 109–113.
• Çoban, T.A., Beydemir, Ş., Gülçin, İ., Ekinci, D. 2007. Morphine inhibits erythrocyte carbonic anhydrase in vitro and in vivo, Biological and Pharmaceutical Bulletin, 30, 2257-2261.
• Çoban, T.A., Beydemir, Ş., Gülçin, İ., Ekinci, D. 2008. The inhibitory effect of ethanol on carbonic anhydrase isozymes: in vivo and in vitro studies, Journal of Enzyme Inhibition and Medicinal Chemistry, 23, 266-270.
• Demir, Y., Nadaroğlu, H., Demir, N. 2004. Effects of omeprazole, famotidine, and ranitidine on the enzyme activities of carbonic anhydrase from bovine stomach in vitro and rat erythrocytes in vivo, Biological and Pharmaceutical Bulletin, 27, 1730-1734.
• Demirdag, R., Yerlikaya, E. and Kufrevioglu, O.I. 2012. Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity, Journal of Enzyme Inhibition and Medicinal Chemistry, 27, 795-9.
• Ekinci, D., Beydemir, S., Alim, Z. 2007. Some drugs inhibit in vitro hydratase and esterase activities of human carbonic anhydrase-I and II, Pharmacological Reports, 59, 580-587.
• Esbaugh, A.J., Tufts, B.L. 2006. The structure and function of carbonic anhydrase isozymes in the respiratory system of vertebrates, Respiratory Physiology and Neurobiology, 154, 185-198.
• Evenepoel, P., Laleman, W., Wilmer, A., Claes, K., Kuypers, D., Bammens, B., Nevens, F., Vanrenterghem, Y. 2006. Prometheus Versus Molecular Adsorbents Recirculating System: Comparison Of Efficiency In Two Different Liver Detoxification Devices, Artificial Organs, 30, 276–284.
• Hewett-Emmett, D., Tashian, R.E. 1996. Functional diversity, conservation, and convergence in the evolution of the α-, ß-, and γ-carbonic anhydrase gene families, Molecular Phylogenetics and Evolution, 5, 50-77.
• Hisar, O., Beydemir, Ş., Gülçin, İ., Küfrevioğlu, Ö.İ., Supuran, C.T. 2005. Effect of low molecular weight plasma inhibitors of rainbow trout (Oncorhyncytes mykiss) on human erythrocytes carbonic anhydrase-II isozyme activity in vitro and rat erythrocytes in vivo, Journal of Enzyme Inhibition and Medicinal Chemistry, 20, 35-39.
• Holten, K.B., Onusko, E.M. 2000. Appropriate prescribing of oral beta-lactam antibiotics, American Family Physician, 62, 611-620.
• Innocenti, A., Gülçin, İ., Scozzafava, A., Supuran, C.T. 2010a. Carbonic anhydrase inhibitors. Antioxidant polyphenol natural products effectively inhibit mammalian isoforms I-XV, Bioorganic and Medicinal Chemistry Letters, 20, 5050-5053.
• Innocenti, A., Öztürk, Sarıkaya, S.B., Gülçin, İ., Supuran, C.T. 2010b. Carbonic anhydrase inhibitors, Inhibition of mammalian isoforms I-XIV with a series of natural product polyphenols and phenolic acids, Bioorganic and Medicinal Chemistry, 18, 2159-2164.
• Kmieć, Z. 2001. Cooperation of liver cells in health and disease. Advances in Anatomy, Embryology and Cell Biology, 161, 1-151.
• Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685.
• Lineweaver, H., Burk, D.J. 1934. The determination of enzyme dissociation constants, Journal of the American Chemical Society, 57, 666-685.
• Meldrum, N.U., Roughton, F.J.W. 1933. Carbonic anhydrase; Its preparation and properties, The Journal of Physiology, 80, 113– 142.
• Neu, H.C. 1982. Antistaphylococcal penicillins, Medical Clinics of North America, 66, 51-60.
• Ottlecz, A., Romero, J.J., Lichtenberger, L.M. 1999. Effect of ranitidine bismuth citrate on the phospholipase A2 activity of Naja naja venom and Helicobacter pylori: a biochemical analysis, Alimentary Pharmacology and Therapeutics, 13, 875-888.
• Öztürk, Sarıkaya, S.B., Gülçin, İ., Supuran, C.T. 2010. Carbonic anhydrase inhibitors, Inhibition of human erythrocyte isozymes I and II with a series of phenolic acids, Chemical Biology and Drug Design, 75, 515-520.
• Prince, A.S., Neu, H.C. 1983. New penicillins and their use in pediatrics, Pediatric Clinics of North America, 30, 33-39.
• Senturk, M., Gulcin, I., Ciftci, M. and Kufrevioglu, O.I. 2008. Dantrolene inhibits human erythrocyte glutathione reductase, Biological and Pharmaceutical Bulletin, 31, 2036-2039.
• Stadie, W.C., O'Brien, H. 1933. The catalysis of the hydration of carbon dioxide and dehydration of carbonic acid by an enzyme isolated from red blood cells, The Journal of Biological Chemistry, 103, 521-529.
• Supuran, C.T., Scozzafava, A., Conway, J. 2004. Carbonic anhydrase- its inhibitors and activators, CRC Press.
• Supuran, C.T., Scozzafava, A. 2000. Carbonic anhydrase inhibitors and their therapeutic potential, Expert Opinion on Therapeutic Patents, 10, 575-600.
• Tashian, R.E., Hewett-Emmett, D., Carter, N., Bergenhem, N.C.H. 2000. Carbonic anhydrase (CA)-related proteins (CA-RPs), and transmembrane proteins with CA or CA-RP domains, In: Chegwidden WR, Carter N, Edwards YH (Eds.), The Carbonic Anhydrases, New Horizons, 105-120.
• Verpoorte, J.A., Mehta, S., Edsall, J.T.J. 1967. Esterase activities of human carbonic anhydrases B and C, The Journal of Biological Chemistry, 242, 4221-4229.
• Veys, E.M. 1991. 20 years experience of ketoprofen, Scandinavian
• Journal of Rheumatology, 90, 1-44.