Gökkuşağı Alabalıklarından İzole Edilen Potansiyel Probiyotik Bakterilerin Hidrofobisite, Safra ve pH Töleranslarının Tespiti

Yıl 2018, , 5 - 11, 01.07.2018

Behire İşıl Didinen Alper Çiftci , Esengül Morsümbül

https://doi.org/10.35864/evmd.512880

Öz

Bu çalışmada, gökkuşağı alabalıklarının bağırsaklarından izole edilen bakterilerin, Lactococcus garvieae’ye karşı in vitro antagonsitik etkileri, hidrofobisiteleri, safra tuzlarına ve düşük pH şartlarına töleransları araştırıldı. Bu amaçla sağlıklı 20 adet gökkuşağı alabalığının (250 g) bağırsaklarından bakterilerilerin izolasyonu yapıldı. İzole edilen 157 adet bakterinin Agar Kuyu Difüzyon yöntemi kullanılarak L. garvieae’ya karşı antagonistik aktiviteleri belirlendi. Dört izolat L. garviea’ye karşı in vitro antagonistik etki gösterdi. Antagonistik suşların fenotipik özelliklerinin saptanmasında geleneksel testler, API 20 NE ve API STREP test kitleri; moleküler identifiksyon için, L. garvieae spesifik PCR ve 16S rRNA gen sekans analizi kullanıldı. Çalışmanın sonucunda, aday probiyotik suşlar, Aeromonas sobria (TUB/2013/L63), Bacillus sp. (TUB/2013/L97) ve L.garvieae (TUB/2013/L32) hidrofobik (bağırsak epitel hücrelerine tutunma kabiliyetinde), safra tuzlarına ve düşük pH şartlarına(balığın sindirim sisteminde hayatta kalma özelliğinde) töleranslı bulundu.

Anahtar Kelimeler

Aeromonas, Bacillus, Lactococcus garvieae, Oncorhyncus mykiss, Probiyotik, hidrofobisite, safra, pH

Kaynakça

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