Piliç Boyun Derilerinden İzole Edilen C. perfringens’lerin Antibiyotik Direnç Profillerinin Belirlenmesi

Yıl 2016, Cilt: 27 Sayı: 2, 93 - 97, 15.12.2016

Güzin İplikçioğlu Çil , Fatma Seda Bilir Ormancı

https://doi.org/10.35864/evmd.514493

Öz

Mikroorganizmaların antibiyotiklere karşı geliştirdikleri direnç, halen tüm dünyada önemini koruyan bir halk sağlığı sorunudur. Antibiyotik direnç ile mücadelede patojen mikroorganizmaların, gıdalarda güvenilir metotlarla tespiti, identifikasyonu ve antibiyotik direnç profillerinin belirlenmesi büyük önem taşımaktadır. Bu çalışmanın amacı, üç farklı kanatlı kesimhanesinden alınan 180 piliç boyun derisi örneğinden izole edilen 35 Clostridium perfringens suşunun antibiyotik direnç profillerinin belirlenmesidir. İzolatların antibiyotik direnç profillerinin tespitinde CLSI (Clinical and Laboratory Standards Institute) tarafından bildirilen disk difüzyon metodu kullanılmıştır. İzolatların 35’inin de (%100) en az bir antibiyotiğe dirençli olduğu, ayrıca 31 (% 88,5) C. perfringens izolatının 1’den fazla antibiyotiğe direnç gösterdiği tespit edilmiştir. İzolatların tamamının (% 100) dirençli olduğu antibiyotik trimetoprim olarak belirlenmiş, bu antibiyotik yanında, % 65,7 ile tetrasiklin, % 62,8 oranında gentamisin direncin yüksek oranda tespit edildiği diğer antibiyotikler olmuştur. Elde edilen veriler izolatların hepsinin vankomisine duyarlı olduğunu da ortaya koymuştur.

Anahtar Kelimeler

Antibiyotik direnç, C. perfringens, disk difüzyon, piliç eti

Kaynakça

• 1. Anonim, (2003). CLSI (Clinical and Laboratory Standards Institute), Methods for antimicrobial susceptibility testing of anaerobic bacteria, Volume 24 Number 2 ISSN 0273-3099 Approved Standard-Sixth Edition. M11-A6 Pennsylvania. USA.
• 2. Bhunia AK, (2008). Foodborne Microbial Pathogens. Mechanisms and Pathogenesis. New York: Springer Publishing, p. 158.
• 3. Bogaard AE, Stobberingh EE, (1999). Antibiotic usage in animals impact on bacterial resistance and public health. Drugs. 58(4), 589-607.
• 4. Capita R, Alonso-Calleja C, (2013). Antibiotic-resistant bacteria: a challenge for the food industry. Critical Rev Food Sci Nut. 53(1), 11-48.
• 5. Casewell M, Friis C, Marco E, McMullin P, Phillips I, (2003). The European ban on growth-promoting antibiotics and emerging consequences for human and animal health. J Antimicrob Chemother. 52, 159-161.
• 6. Ellie JC, Goldstein MD, Diane M, (2011). Resistance trends in antimicrobial susceptibility of anaerobic bacteria, part 1. Clin Microbiol Newsl. 33(1), 1-8.
• 7. Erol İ, (2007). Gıda Hijyeni ve Mikrobiyolojisi. Ankara: Pozitif Matbaacılık, p.154.
• 8. Gaskins HR, Collier CT, Anderson DB, (2002). Antibiotics as growth promotants: mode of action. Anim Biotechnol. 13(1), 29-42.
• 9. Hecht DW, Vedantam G, Osmolskı JR, (1999). Antibiotic resistance among anaerobes: what does it mean?. Anaerobe. 5, 421-429.
• 10. Johansson A, Greko C, Engström BE, Karlsson M, (2004). Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of Clostridium perfringens from poultry, and distribution of tetracycline resistance genes. Vet Microbiol. 99, 251-257.
• 11. Khan M, Nazir J, Anjum AA, Nawaz M, Shabbir MZ, (2015). Toxinotyping and antimicrobial susceptibility of enterotoxigenic Clostridium perfringens isolates from mutton, beef and chicken meat. J Food Sci Tech. 52(8), 5323- 5328.
• 12. Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Greko C, (2013). Antibiotic resistance— the need for global solutions. Lancet Infect Dis, 13(12), 1057-1098.
• 13. Martel A, Devrıese La, Cauwerts K, De Gussem K, Decostere A, Haesebrouck F, (2004). Susceptibility of Clostridium perfringens strains from broiler chickens to antibiotics and anticoccidials. Avian Pathol. 33(1), 3-7.
• 14. McDermott PF, Zhao S, Wagner DD, Simjee S, Walker RD, White DG, (2002). The food safety perspective of antibiotic resistance. Anim Biotech. 13(1), 71-84.
• 15. McEwen SA, Fedorka-Cray PJ, (2002). Antimicrobial use and resistance in animals. Clin Infect Dis. 34(3), 93-106.
• 16. Novak JS, Juneja VK, (2002). Clostridium perfringens: hazards in new generation foods. Innov Food Sci Emerg. 3(2), 127-132.
• 17. Nowell VJ, Poppe C, Parreira VR, Jiang Y, Reid-Smith R, Prescott JF, (2010). Clostridium perfringens in retail chicken. Anaerobe. 16, 314-315.
• 18. Rasmussen BA, Bush K, Tally FP, (1997). Antimicrobial resistance in anaerobes. Clin Infect Dis. 24(1), 110-120.
• 19. Rood JI, Maher EA, Somers EB, Campos E, Duncan CL, (1978). Isolation and characterization of multiply antibiotic- resistant Clostridium perfringens strains from porcine feces. Antimicrob Agents Chemother. 13(5), 871-880.
• 20. Silva ROS, Salvaranı FM, Assis RA, Martins NRS, Pires PS, Lobato FCF, (2009). Antimicrobial susceptibility of Clostridium perfringens strains isolated from broiler chickens. Braz. J Microbiol. 40(2), 262-264.
• 21. Singh RV, Bhilegaonkar KN, Agarwal RK, (2005). Studies on occurrence and characterization of Clostridium perfringens from select meats. J Food Safety. 25, 146-156.
• 22. Tansuphasiri U, Matra W, Sangsuk L, (2005). Antimicrobial resistance among Clostridium perfringens isolated from various sources in Thailand Southeast Asian. J Trop Med Public Health. 36(4), 954-961.
• 23. Teuber M, (1999). Spread of antibiotic resistance with foodborne pathogens. Cell Mol Life Sci. 56, 755-763.
• 24. Watkins KL, Shryock TR, Dearth, RN, Saif, YM, (1997). In-vitro antimicrobial susceptibility of Clostridium perfringens from commercial turkey and broiler chicken origin. Vet Microbiol. 54, 195-200.